Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   FLK62_RS03315 Genome accession   NZ_CP041334
Coordinates   677843..679105 (+) Length   420 a.a.
NCBI ID   WP_005712502.1    Uniprot ID   -
Organism   Glaesserella parasuis strain HPS412     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672843..684105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLK62_RS03290 (FLK62_03320) - 673037..675592 (-) 2556 WP_035498541.1 penicillin-binding protein 1A -
  FLK62_RS03295 (FLK62_03325) comA 675746..676423 (+) 678 WP_176443493.1 competence protein ComA Machinery gene
  FLK62_RS03300 (FLK62_03330) comB 676399..676920 (+) 522 WP_176443494.1 hypothetical protein Machinery gene
  FLK62_RS03305 (FLK62_03335) comC 676917..677447 (+) 531 WP_015940159.1 hypothetical protein Machinery gene
  FLK62_RS03310 (FLK62_03340) comD 677492..677833 (+) 342 WP_235685072.1 hypothetical protein Machinery gene
  FLK62_RS03315 (FLK62_03345) comE 677843..679105 (+) 1263 WP_005712502.1 type IV pilus secretin PilQ Machinery gene
  FLK62_RS03320 (FLK62_03350) nusB 679184..679597 (+) 414 WP_010785952.1 transcription antitermination factor NusB -
  FLK62_RS03325 (FLK62_03355) thiL 679606..680574 (+) 969 WP_176443495.1 thiamine-phosphate kinase -
  FLK62_RS03330 (FLK62_03360) - 680577..681047 (+) 471 WP_005712497.1 phosphatidylglycerophosphatase A -
  FLK62_RS03335 (FLK62_03365) - 681049..681678 (+) 630 WP_010785950.1 LysE family transporter -
  FLK62_RS03340 (FLK62_03370) trxA 681975..682289 (+) 315 WP_005712493.1 thioredoxin -
  FLK62_RS03345 (FLK62_03375) - 682835..683482 (+) 648 WP_176443496.1 transposase -
  FLK62_RS03350 (FLK62_03380) - 683673..684092 (-) 420 WP_005714137.1 hotdog fold thioesterase -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 47051.05 Da        Isoelectric Point: 8.9182

>NTDB_id=371276 FLK62_RS03315 WP_005712502.1 677843..679105(+) (comE) [Glaesserella parasuis strain HPS412]
MRYLFLLFFATFPVLANQQISLSIKNAPTAEIIGYLAEETGKNITISDEIKDTKNFRVEKSHFDEILNSLIKTHQLNLKK
ENGIYYIHQAQEHKQHTTAQLVNALPKLITKTIKLHYSKASEVIESLTKGQGNLLSENGYLHFDDRSNSIIVKDSAASVK
NFTQLIETLDKPTEQIAIEARIVTISSEHLQQLGVRWGLFSPNENHYKLAGNLEGNGLTTNNLNVNFPVNPSASVALQIA
AINSRVLHLELTALESENNIEIIASPRLLTTDKKPASIKQGTEIPYAMYSKKKEITDIEFREAVLGLEVTPHISKQNQIL
LDLAISQNSPNNQMNNMMATIDKQEINTQVLAKHGETIVLGGIFQHLIAKGEDKVPLLGSIPVIKRLFSQNRDKISKREL
VIFVTPYIVKSEKMGAEKQK

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=371276 FLK62_RS03315 WP_005712502.1 677843..679105(+) (comE) [Glaesserella parasuis strain HPS412]
ATGCGTTATTTATTCCTGCTATTCTTTGCTACTTTTCCTGTATTAGCAAACCAACAAATTTCGCTTTCTATAAAAAATGC
CCCTACGGCAGAAATTATTGGTTATTTAGCTGAAGAAACGGGAAAAAATATTACGATTTCAGATGAGATTAAAGATACAA
AAAATTTCAGAGTAGAAAAAAGCCATTTTGATGAGATATTAAATAGTTTAATCAAAACACATCAATTAAATTTAAAAAAA
GAAAATGGCATTTACTATATTCATCAAGCCCAAGAACATAAACAACATACTACGGCACAATTAGTTAATGCTCTGCCTAA
ATTAATCACCAAAACAATCAAGTTACACTATTCCAAAGCCTCTGAAGTAATAGAGTCTTTGACAAAAGGGCAAGGCAACT
TGCTATCAGAGAATGGTTATCTTCATTTTGATGATCGCAGTAATAGTATTATCGTCAAAGACAGTGCCGCATCGGTTAAA
AACTTTACTCAACTTATTGAAACCCTAGATAAACCAACGGAACAAATTGCAATTGAAGCCCGAATTGTCACGATCAGCAG
TGAACATTTACAACAACTTGGCGTGCGTTGGGGCTTATTTTCCCCTAACGAAAATCACTACAAATTGGCAGGAAACTTAG
AGGGCAACGGATTAACTACCAACAACTTAAACGTAAATTTTCCAGTAAATCCGTCCGCTTCCGTTGCCTTACAAATTGCC
GCTATCAACAGCCGTGTACTTCATTTAGAACTCACCGCATTAGAGAGCGAAAATAACATTGAGATCATTGCAAGCCCTCG
CTTACTCACAACAGATAAAAAACCAGCGAGTATCAAACAAGGTACAGAGATTCCTTATGCAATGTACAGTAAGAAAAAGG
AAATCACCGATATTGAATTTCGTGAAGCGGTTTTGGGGCTAGAAGTCACGCCACATATTTCTAAACAAAATCAGATTTTG
TTAGATCTTGCCATTAGCCAAAATTCGCCAAATAACCAGATGAACAATATGATGGCAACGATTGATAAACAAGAAATTAA
TACACAAGTCCTTGCTAAACACGGCGAAACCATCGTATTAGGCGGTATTTTTCAACATCTGATCGCCAAAGGCGAAGATA
AAGTCCCGCTGTTAGGCAGTATCCCCGTGATTAAACGCTTATTTAGCCAAAACCGAGATAAAATCTCTAAACGGGAGCTG
GTTATTTTTGTTACGCCTTATATTGTAAAATCTGAAAAAATGGGAGCGGAAAAGCAGAAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(110-175)

Bacterial type II and III secretion system protein (Secretin)

(253-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

96.667

100

0.967
  comE Haemophilus influenzae Rd KW20

52.706

100

0.533
  comE Haemophilus influenzae 86-028NP

51.765

100

0.524
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.44

98.571

0.369
  pilQ Vibrio cholerae strain A1552

37.44

98.571

0.369
  pilQ Vibrio campbellii strain DS40M4

36.019

100

0.362

Multiple sequence alignment