Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3M73_RS10645 Genome accession   NZ_CP048605
Coordinates   2252611..2253996 (+) Length   461 a.a.
NCBI ID   WP_163425234.1    Uniprot ID   -
Organism   Escherichia coli strain PapRG-06-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2247611..2258996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M73_RS10620 (G3M73_10620) aroP 2248159..2249532 (+) 1374 WP_075842203.1 aromatic amino acid transporter AroP -
  G3M73_RS10625 (G3M73_10625) ampE 2249575..2250429 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  G3M73_RS10630 (G3M73_10630) ampD 2250426..2250977 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G3M73_RS10635 (G3M73_10635) nadC 2251065..2251958 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G3M73_RS10640 (G3M73_10640) ppdD 2252161..2252601 (+) 441 WP_000360900.1 prepilin peptidase-dependent pilin -
  G3M73_RS10645 (G3M73_10645) pilB 2252611..2253996 (+) 1386 WP_163425234.1 type II secretion system protein GspE Machinery gene
  G3M73_RS10650 (G3M73_10650) hofC 2253986..2255188 (+) 1203 WP_163425235.1 protein transport protein HofC -
  G3M73_RS10655 (G3M73_10655) guaC 2255223..2256266 (-) 1044 WP_130204936.1 GMP reductase -
  G3M73_RS23155 - 2256422..2256466 (-) 45 WP_120795372.1 protein YacM -
  G3M73_RS10660 (G3M73_10660) coaE 2256491..2257111 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  G3M73_RS10665 (G3M73_10665) zapD 2257111..2257854 (+) 744 WP_001194722.1 cell division protein ZapD -
  G3M73_RS10670 (G3M73_10670) yacG 2257864..2258061 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G3M73_RS10675 (G3M73_10675) mutT 2258500..2258898 (-) 399 WP_053291182.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50622.30 Da        Isoelectric Point: 6.5405

>NTDB_id=369946 G3M73_RS10645 WP_163425234.1 2252611..2253996(+) (pilB) [Escherichia coli strain PapRG-06-5]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHTSRTLQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=369946 G3M73_RS10645 WP_163425234.1 2252611..2253996(+) (pilB) [Escherichia coli strain PapRG-06-5]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACACCAGTCGCACACTACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CAGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-65)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371