Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E8E07_RS16955 Genome accession   NZ_CP040911
Coordinates   3477158..3478870 (+) Length   570 a.a.
NCBI ID   WP_086400327.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AB17H194     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3472158..3483870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8E07_RS16920 nusA 3473046..3474530 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  E8E07_RS16925 rimP 3474568..3475092 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  E8E07_RS16945 secG 3475730..3476059 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  E8E07_RS16950 tpiA 3476072..3476866 (-) 795 WP_017398730.1 triose-phosphate isomerase -
  E8E07_RS16955 pilB 3477158..3478870 (+) 1713 WP_086400327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E8E07_RS16960 pilC 3478897..3480123 (+) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  E8E07_RS16965 pilD 3480123..3480983 (+) 861 WP_086400328.1 prepilin peptidase Machinery gene
  E8E07_RS16970 coaE 3480985..3481581 (+) 597 WP_086400329.1 dephospho-CoA kinase -
  E8E07_RS16975 - 3481578..3482492 (-) 915 WP_086400330.1 DMT family transporter -
  E8E07_RS16980 rlmB 3482528..3483277 (-) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  E8E07_RS16985 - 3483380..3483706 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63501.85 Da        Isoelectric Point: 5.0248

>NTDB_id=368265 E8E07_RS16955 WP_086400327.1 3477158..3478870(+) (pilB) [Acinetobacter pittii strain AB17H194]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEDTPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=368265 E8E07_RS16955 WP_086400327.1 3477158..3478870(+) (pilB) [Acinetobacter pittii strain AB17H194]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGTAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGTAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCCCCCTCACAAGAGGATGAGGAAGACACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCGTACGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGATGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAACTGGCTAACCGTTTAGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAGCGGGTACCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACAGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAAGTTAACGTTAACCCGA
AAGTAGGTCTTACTTTTTCAGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCGCAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
GCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTCAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTTACTTCC
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.561

100

0.946
  pilB Acinetobacter baylyi ADP1

78.246

100

0.782
  pilB Legionella pneumophila strain ERS1305867

51.499

99.474

0.512
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

51.908

91.93

0.477
  pilB Vibrio campbellii strain DS40M4

51.06

91.053

0.465
  pilB Vibrio cholerae strain A1552

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.342

100

0.381

Multiple sequence alignment