Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   E8E07_RS16965 Genome accession   NZ_CP040911
Coordinates   3480123..3480983 (+) Length   286 a.a.
NCBI ID   WP_086400328.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AB17H194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3475123..3485983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8E07_RS16945 secG 3475730..3476059 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  E8E07_RS16950 tpiA 3476072..3476866 (-) 795 WP_017398730.1 triose-phosphate isomerase -
  E8E07_RS16955 pilB 3477158..3478870 (+) 1713 WP_086400327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E8E07_RS16960 pilC 3478897..3480123 (+) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  E8E07_RS16965 pilD 3480123..3480983 (+) 861 WP_086400328.1 prepilin peptidase Machinery gene
  E8E07_RS16970 coaE 3480985..3481581 (+) 597 WP_086400329.1 dephospho-CoA kinase -
  E8E07_RS16975 - 3481578..3482492 (-) 915 WP_086400330.1 DMT family transporter -
  E8E07_RS16980 rlmB 3482528..3483277 (-) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  E8E07_RS16985 - 3483380..3483706 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  E8E07_RS16990 - 3483783..3485093 (-) 1311 WP_086400331.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32126.66 Da        Isoelectric Point: 7.5057

>NTDB_id=368267 E8E07_RS16965 WP_086400328.1 3480123..3480983(+) (pilD) [Acinetobacter pittii strain AB17H194]
MQEIIAYFIQNLTALYIAVALLSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPELPIIEHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLMLRGKCGHCQHPISIRYPAIELLTMLCSLVVVMVFGPTLQMLWGLVLTWILIALTFIDFDTQLLPDR
FTLPLAALGLGINTFSIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIVGIILLKLRNNNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=368267 E8E07_RS16965 WP_086400328.1 3480123..3480983(+) (pilD) [Acinetobacter pittii strain AB17H194]
ATGCAAGAAATTATTGCTTATTTTATTCAAAACTTAACTGCACTTTATATTGCAGTCGCACTATTGAGCCTATGTATTGG
TAGCTTTTTGAATGTGGTAATTTATCGCACCCCCAAAATGATGGAACAAGATTGGCAGCAGGAATGTCAAATGTTGCTTA
ATCCCGAACTACCCATCATTGAGCATGAAAAGCTGACATTAAGTAAACCTGCTTCATCATGTCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCAGTTATAAGCTGGTTAATGTTAAGAGGAAAATGTGGCCATTGCCAACATCCTATTAG
CATTCGTTATCCAGCGATTGAATTACTCACGATGCTATGTTCATTAGTAGTCGTCATGGTTTTTGGTCCAACCCTACAAA
TGCTTTGGGGGCTAGTGCTCACTTGGATTTTGATTGCTTTAACATTTATAGACTTTGATACTCAGCTACTACCTGACCGT
TTCACCCTACCTTTGGCTGCTCTCGGCTTAGGTATTAATACCTTTAGTATTTACACCTCGCCTAACTCAGCCATTTGGGG
ATATTTAATCGGCTTTTTATGTCTTTGGATTGTGTATTACTTATTTAAAGTGATTACAGGCAAAGAAGGTATGGGTTATG
GCGACTTTAAACTGCTTGCCGCATTAGGCGCGTGGATGGGGCCATTGATGCTGCCATTAATTGTGCTATTGTCATCTTTA
CTAGGTGCAATTGTTGGCATCATTTTACTAAAATTGCGCAATAATAATCAGCCCTTTGCGTTTGGGCCCTATATTGCCAT
TGCGGGTTGGGTTGCCTTTTTATGGGGTGACCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

95.455

100

0.955
  pilD Acinetobacter nosocomialis M2

95.105

100

0.951
  pilD Vibrio cholerae strain A1552

54.212

95.455

0.517
  pilD Vibrio campbellii strain DS40M4

53.309

95.105

0.507
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437

Multiple sequence alignment