Detailed information NTG0367748

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: FHQ07_RS05755
• Genome accession: NZ_CP040871
• Coordinates: 1181772..1183490 (+)
• Length: 572 a.a.
• NCBI ID: WP_139715908.1
• Uniprot ID: A0A5B7ZQV7
• Organism: Thermomonas aquatica strain SY21
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1175681..1191728	1181772..1183490	within	0

Gene organization within MGE regions

Location: 1175681..1191728

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FHQ07_RS05720 (FHQ07_05720)	-	1175681..1176859 (+)	1179	WP_139715903.1	class I SAM-dependent methyltransferase	-
FHQ07_RS14315	-	1176868..1177410 (+)	543	WP_168191471.1	GNAT family protein	-
FHQ07_RS05730 (FHQ07_05730)	-	1177407..1178147 (+)	741	WP_139715904.1	WbqC family protein	-
FHQ07_RS05735 (FHQ07_05735)	-	1178119..1178853 (+)	735	WP_240703555.1	CmcI family methyltransferase	-
FHQ07_RS05740 (FHQ07_05740)	-	1178893..1179783 (+)	891	WP_139715905.1	glycosyltransferase family A protein	-
FHQ07_RS05745 (FHQ07_05745)	-	1179783..1180772 (+)	990	WP_139715906.1	hypothetical protein	-
FHQ07_RS05750 (FHQ07_05750)	-	1180769..1181650 (-)	882	WP_139715907.1	glycosyltransferase family 2 protein	-
FHQ07_RS05755 (FHQ07_05755)	pilB	1181772..1183490 (+)	1719	WP_139715908.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FHQ07_RS05760 (FHQ07_05760)	pilC	1183502..1184773 (+)	1272	WP_139715909.1	type II secretion system F family protein	Machinery gene
FHQ07_RS05765 (FHQ07_05765)	pilD	1184792..1185655 (+)	864	WP_139715910.1	A24 family peptidase	Machinery gene
FHQ07_RS05770 (FHQ07_05770)	coaE	1185676..1186299 (+)	624	WP_139715911.1	dephospho-CoA kinase	-
FHQ07_RS05775 (FHQ07_05775)	-	1186350..1187312 (-)	963	WP_139715912.1	Nudix family hydrolase	-
FHQ07_RS05780 (FHQ07_05780)	secA	1187371..1190097 (-)	2727	WP_139715913.1	preprotein translocase subunit SecA	-
FHQ07_RS05785 (FHQ07_05785)	-	1190244..1191200 (-)	957	WP_240703556.1	M23 family metallopeptidase	-
FHQ07_RS14660	-	1191225..1191728 (+)	504	WP_338419631.1	DciA family protein	-

Sequence

Protein

• Length: 572 a.a.
• Molecular weight: 62190.47 Da
• Isoelectric Point: 6.2547

>NTDB_id=367748 FHQ07_RS05755 WP_139715908.1 1181772..1183490(+) (pilB) [Thermomonas aquatica strain SY21]
MSAVTSANLMGITGIARRLVLDGAMDEGAARRAMDAATAAKVPLATYIADKRLADPGALAAAYSMEFGMPLVDPLAIDPG
QSAMRLVKEELVRKHQVLPLFKRGSRLFVGVSDPTDHGALDEIKFHTNLTVEPILIDAERLKRSIDGWLEAAEDLADADN
DGLEGLDVSGGDEDLSNDSGVDAGGDDTPVVKFVNKVLVDAIRKGASDIHFEPYETEYRVRLRIDGILKTVKRVPVKLHQ
RIAARVKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVMRILDGSAAKLGIDKLGYEQVQQKLFLDA
IHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTVEDPVEIRLPGVNQVQQNTKRGMTFAAALRSFLRQDPDIIMV
GEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVSLVIAQRLVRRLHDCKRAVHLPEHALLA
EGFTADEVHAGITLYEAVGCEDCTEGYKGRSGVYQVMPMTDEIQQIVLAGGNVQQITEAALISGVRDLRRSALDKAKNGV
TSLVEINRVTKD

Nucleotide

• Length: 1719 bp

>NTDB_id=367748 FHQ07_RS05755 WP_139715908.1 1181772..1183490(+) (pilB) [Thermomonas aquatica strain SY21]
ATGAGCGCAGTCACGTCCGCCAATCTGATGGGCATTACCGGCATTGCGCGGCGCCTGGTACTGGACGGCGCCATGGACGA
AGGTGCGGCGCGCAGGGCGATGGATGCGGCAACAGCGGCGAAAGTACCGCTGGCGACTTATATCGCGGACAAGCGCTTGG
CGGATCCCGGGGCGCTTGCGGCAGCCTATTCCATGGAATTCGGCATGCCGCTGGTGGATCCCCTGGCGATCGACCCCGGT
CAATCCGCGATGCGGCTGGTCAAGGAGGAACTGGTGCGCAAGCACCAGGTACTGCCACTGTTCAAGCGCGGCAGTCGCCT
ATTTGTCGGCGTTTCCGACCCGACCGACCATGGTGCGCTGGACGAAATCAAGTTCCACACCAACCTGACAGTCGAGCCGA
TATTGATCGATGCAGAGCGCCTGAAGCGCAGCATCGATGGCTGGCTGGAGGCGGCCGAGGATCTCGCCGACGCCGACAAC
GACGGCCTGGAGGGCCTGGACGTCAGCGGCGGGGACGAGGATCTCAGCAACGATTCCGGCGTGGATGCCGGCGGGGATGA
CACCCCGGTGGTCAAGTTCGTCAATAAGGTGCTGGTGGACGCCATCCGCAAGGGCGCATCGGACATCCATTTCGAACCCT
ACGAAACCGAATACCGGGTCAGGCTGCGCATCGACGGCATCCTGAAGACGGTCAAGCGTGTGCCGGTCAAGCTTCACCAG
CGCATCGCCGCGCGCGTCAAGGTCATGGCCCAGTTGGACATCGCCGAGAAGCGGGTACCACAGGACGGGCGCATCAAGCT
GAACCTGTCCAAGACCAAGCAGATCGATTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGTGAGAAGGTGGTGATGCGCA
TCCTGGACGGCAGTGCCGCAAAGTTGGGCATCGACAAGCTCGGCTACGAGCAGGTCCAGCAGAAACTCTTCCTGGATGCG
ATCCACAAGCCCTATGGCATGGTGCTGGTTACCGGCCCAACCGGCTCGGGCAAGACGGTGAGCCTGTACACCGCGCTCGG
GATCCTCAACGACGAAACCCGCAACATCAGCACGGTGGAGGATCCGGTCGAGATCCGGCTGCCAGGGGTCAACCAGGTCC
AGCAGAACACCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGAAGCTTCCTGCGCCAGGATCCGGACATCATCATGGTC
GGCGAAATCCGCGACCTGGAAACCGCCGAAATCGCGGTGAAGGCGGCGCAGACCGGCCACATGGTGCTGTCGACGCTTCA
CACAAACGACGCACCGCAGACCATCGCCCGTCTGATGAACATGGGCATCGCGCCCTACAACATCACCAGCTCGGTCAGCC
TGGTGATCGCCCAGCGCCTGGTCCGCCGCCTGCACGATTGCAAACGTGCCGTGCACCTCCCCGAGCATGCGTTGCTCGCC
GAGGGCTTCACCGCCGACGAAGTCCACGCAGGCATCACCCTCTACGAGGCAGTGGGCTGCGAGGATTGCACCGAAGGGTA
CAAGGGCCGTTCGGGCGTCTACCAGGTGATGCCGATGACCGACGAGATCCAGCAGATCGTACTGGCCGGTGGTAATGTCC
AGCAGATCACCGAGGCAGCCTTGATCAGCGGCGTCCGCGACCTGCGCCGGTCTGCGTTGGACAAGGCCAAGAACGGCGTC
ACCAGCCTGGTCGAAATCAACCGAGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (51-148)

• Type II/IV secretion system protein (T2SSE) (196-463)

3D structure

• AlphaFold DB: A0A5B7ZQV7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	52.962	100	0.531
pilB	Acinetobacter baumannii D1279779	53.274	98.776	0.526
pilB	Legionella pneumophila strain ERS1305867	52.465	99.301	0.521
pilB	Vibrio cholerae strain A1552	49.389	100	0.495
pilF	Neisseria gonorrhoeae MS11	48.313	98.427	0.476
pilB	Vibrio parahaemolyticus RIMD 2210633	48.659	91.259	0.444
pilB	Vibrio campbellii strain DS40M4	50.102	85.839	0.43
pilF	Thermus thermophilus HB27	40.347	90.559	0.365