Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FGD67_RS13750 Genome accession   NZ_CP040755
Coordinates   3019957..3021663 (-) Length   568 a.a.
NCBI ID   WP_257171701.1    Uniprot ID   -
Organism   Colwellia sp. M166     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3014957..3026663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGD67_RS13720 (FGD67_13595) mutT 3015159..3015557 (+) 399 WP_257171696.1 8-oxo-dGTP diphosphatase MutT -
  FGD67_RS13725 (FGD67_13600) yacG 3015585..3015821 (-) 237 WP_257171697.1 DNA gyrase inhibitor YacG -
  FGD67_RS13730 (FGD67_13605) zapD 3015977..3016723 (-) 747 WP_257171698.1 cell division protein ZapD -
  FGD67_RS13735 (FGD67_13610) coaE 3016818..3017429 (-) 612 WP_257171699.1 dephospho-CoA kinase -
  FGD67_RS13740 (FGD67_13615) pilD 3017480..3018364 (-) 885 WP_373567771.1 A24 family peptidase Machinery gene
  FGD67_RS13745 (FGD67_13620) pilC 3018523..3019770 (-) 1248 WP_257171700.1 type II secretion system F family protein Machinery gene
  FGD67_RS13750 (FGD67_13625) pilB 3019957..3021663 (-) 1707 WP_257171701.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FGD67_RS13755 (FGD67_13630) - 3021789..3022256 (-) 468 WP_306556754.1 pilin -
  FGD67_RS13760 (FGD67_13635) nadC 3022691..3023578 (-) 888 WP_257171702.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FGD67_RS13765 (FGD67_13640) - 3023579..3024100 (-) 522 WP_257171703.1 TIGR02281 family clan AA aspartic protease -
  FGD67_RS13770 (FGD67_13645) ampD 3024198..3024779 (+) 582 WP_373567884.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FGD67_RS13775 (FGD67_13650) ampE 3024918..3025799 (+) 882 WP_257171705.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62466.65 Da        Isoelectric Point: 5.0623

>NTDB_id=367031 FGD67_RS13750 WP_257171701.1 3019957..3021663(-) (pilB) [Colwellia sp. M166]
MKGLHQQSSLLSALAKHDIVTREMVDTISADFIRQKKPFVRYLVEDKNINAEQIASTLSRSFGCPLIDLSNFDTSLVPEG
VRNEKLVRKHSALPLFLRGKVLFVAMSDPTNHDALEEIQFNTGFSTELVLANETSLMACIEKILEADSDALDITDMDTAE
LAGIDVQDEHQEDDNIGGGDKEDAPIVVYINKILLDAIRKGASDLHFEPYEKSYRIRFRIDGILTEVAKPPVSLASRMAA
RLKVMSKLDIAERRVPQDGRIKLALSKKKSIDFRVSTLPTMWGEKVVMRILDSSSAMLGIEMLGYAPEQKQIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVQINTRAGLTFPGALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSVIIAQRLARRLCIQCKTEENIPESELLTQGF
LPEQLSTIKLYKPVGCELCTGGYKGRVGIYEVIKISDVTANLIMTGSNSLDIAAQCQREGYNNLRQSGLEKAALGVTSLE
EVNRVTNN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=367031 FGD67_RS13750 WP_257171701.1 3019957..3021663(-) (pilB) [Colwellia sp. M166]
ATGAAAGGTCTTCATCAACAATCAAGCCTGCTATCGGCACTCGCCAAACATGATATTGTTACCCGTGAGATGGTCGACAC
TATAAGTGCTGACTTTATTCGCCAAAAAAAACCATTTGTTCGTTACCTAGTCGAAGATAAAAATATTAATGCCGAACAAA
TAGCTAGCACACTCTCCCGCAGTTTTGGCTGTCCTCTGATTGATCTGAGTAATTTTGACACATCATTAGTCCCTGAAGGT
GTCCGCAATGAGAAGCTGGTGCGCAAACACAGTGCCTTACCTTTATTCTTGCGTGGCAAGGTATTATTTGTTGCCATGTC
GGACCCAACTAACCATGATGCGCTAGAAGAAATTCAGTTTAATACCGGTTTTTCAACTGAATTAGTCCTTGCTAATGAAA
CCTCATTAATGGCTTGCATCGAGAAAATTTTAGAAGCAGACAGCGATGCCCTTGATATCACCGATATGGATACTGCCGAG
TTAGCTGGTATCGATGTGCAAGATGAACATCAAGAAGACGATAACATTGGCGGTGGCGATAAAGAAGATGCTCCTATAGT
TGTTTATATTAACAAAATATTACTCGACGCTATTCGTAAAGGCGCCTCTGATTTACATTTTGAACCTTATGAAAAATCTT
ACCGTATTCGCTTTCGTATCGATGGCATATTAACCGAAGTCGCTAAACCGCCGGTATCTTTAGCATCACGTATGGCTGCA
CGCCTAAAAGTGATGTCGAAGTTGGATATTGCAGAGCGGCGCGTACCACAAGATGGTCGTATAAAGTTGGCGTTATCAAA
AAAGAAATCAATTGATTTTCGTGTTAGTACTCTACCTACTATGTGGGGTGAGAAGGTAGTGATGCGTATTCTTGACTCCT
CCAGCGCCATGCTGGGCATTGAAATGCTTGGTTATGCTCCAGAGCAAAAGCAAATTTACATGGATGCACTAGCACAACCG
CAAGGCATGATATTAGTAACAGGCCCAACCGGCTCGGGAAAAACCGTATCACTTTATACCGGCTTGAATATTTTAAATAC
CGAAGAGCGCAACATTTCCACCGCAGAAGATCCGGTGGAAATTAACCTTGAAGGTGTCAACCAAGTACAAATTAATACCC
GTGCAGGTTTAACCTTTCCGGGGGCATTACGCTCATTTCTCCGTCAAGATCCCGATATTGTTATGGTCGGCGAAATTCGT
GATTTAGAAACCGCTGAAATTGCTATTAAGGCGGCTCAAACCGGTCATTTAGTGCTATCAACTTTACATACTAATTCAGC
AGCAGAAACATTAACTCGCTTATTAAATATGGGCGTACCATCCTATAATGTGGCAAGCTCTGTCAGTGTTATTATTGCCC
AGCGCTTAGCACGTCGATTATGCATTCAGTGCAAAACAGAAGAAAACATTCCCGAAAGCGAACTACTTACACAAGGCTTT
TTACCCGAACAACTTAGTACCATAAAGTTATATAAACCGGTCGGTTGTGAGCTATGTACTGGAGGTTATAAAGGTCGTGT
TGGTATATATGAAGTAATAAAAATTAGTGATGTAACAGCAAATTTGATCATGACGGGCTCTAACTCTTTAGATATTGCCG
CTCAGTGTCAACGTGAAGGCTACAACAATTTACGCCAGTCGGGCCTAGAAAAAGCCGCTTTAGGTGTTACCAGTTTAGAA
GAAGTAAATCGCGTCACTAATAATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.888

94.366

0.565
  pilB Acinetobacter baumannii D1279779

58.256

94.894

0.553
  pilB Legionella pneumophila strain ERS1305867

58.131

94.19

0.548
  pilB Vibrio cholerae strain A1552

51.937

100

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

52.5

91.549

0.481
  pilB Vibrio campbellii strain DS40M4

51.916

91.901

0.477
  pilF Neisseria gonorrhoeae MS11

47.78

99.12

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.513

94.014

0.371

Multiple sequence alignment