Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GSS61_RS15450 Genome accession   NZ_CP047985
Coordinates   3267277..3268779 (+) Length   500 a.a.
NCBI ID   WP_062851604.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain HZ-52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3262277..3273779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSS61_RS15430 (GSS61_16205) hslO 3262744..3263619 (-) 876 WP_005465326.1 Hsp33 family molecular chaperone HslO -
  GSS61_RS15435 (GSS61_16210) hslR 3263651..3264037 (-) 387 WP_005465328.1 ribosome-associated heat shock protein Hsp15 -
  GSS61_RS15440 (GSS61_16215) gspC 3264293..3265216 (+) 924 WP_005465330.1 type II secretion system protein GspC -
  GSS61_RS15445 (GSS61_16220) gspD 3265253..3267277 (+) 2025 WP_193239042.1 type II secretion system secretin GspD -
  GSS61_RS15450 (GSS61_16225) pilB/pilB1 3267277..3268779 (+) 1503 WP_062851604.1 type II secretion system ATPase GspE Machinery gene
  GSS61_RS15455 (GSS61_16230) gspF 3268779..3269996 (+) 1218 WP_005465194.1 type II secretion system inner membrane protein GspF -
  GSS61_RS15460 (GSS61_16235) gspG 3270058..3270501 (+) 444 WP_005465191.1 type II secretion system major pseudopilin GspG -
  GSS61_RS15465 (GSS61_16240) gspH 3270532..3271128 (+) 597 WP_044139892.1 type II secretion system minor pseudopilin GspH -
  GSS61_RS15470 (GSS61_16245) gspI 3271115..3271504 (+) 390 WP_005478722.1 type II secretion system minor pseudopilin GspI -
  GSS61_RS15475 (GSS61_16250) gspJ 3271491..3272174 (+) 684 WP_020839934.1 type II secretion system minor pseudopilin GspJ -
  GSS61_RS15480 (GSS61_16255) gspK 3272167..3273177 (+) 1011 WP_025789352.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 55777.90 Da        Isoelectric Point: 5.9227

>NTDB_id=366792 GSS61_RS15450 WP_062851604.1 3267277..3268779(+) (pilB/pilB1) [Vibrio parahaemolyticus strain HZ-52]
MVDMLDTAPSMRRLPFSFANRFKLVLETEHPERPPILYYVEPLNPAALVEVRRVLKRSFVPQAIDKESFDKKLTEAYQRD
SSEARQLMEDIGADSDDFFSLAEELPHDEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGVLRE
VLTPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTP
SVHDNFRHLIGRPHGIILVTGPTGSGKSTTLYAGLQEINSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAIL
RQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCSDCKEP
YEADSEQKKLFGMAESESLILHRAKGCEACNQKGYRGRTGIHELVLVDETVQELIHREAGEQEVEKAVRNHTPSIRSDGL
SKVRRGFTSLEEVMRVTKEA

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=366792 GSS61_RS15450 WP_062851604.1 3267277..3268779(+) (pilB/pilB1) [Vibrio parahaemolyticus strain HZ-52]
ATGGTAGATATGTTAGACACTGCGCCAAGTATGCGTCGTCTGCCATTCAGTTTTGCCAATCGCTTTAAACTGGTTTTGGA
GACTGAGCATCCTGAGCGTCCTCCAATTCTTTACTACGTGGAGCCGCTAAATCCTGCGGCTCTGGTTGAAGTACGTCGCG
TGCTTAAGCGCAGCTTCGTGCCTCAAGCCATCGACAAAGAATCCTTCGATAAAAAGCTGACTGAAGCGTATCAACGTGAT
TCATCAGAAGCTCGTCAATTGATGGAAGATATCGGTGCCGACAGTGACGATTTCTTCTCGTTGGCAGAAGAGCTACCGCA
TGATGAGGACTTGTTAGAGTCGGAAGACGATGCGCCAATCATCAAATTGATTAATGCCATGTTGGGTGAAGCGATCAAAG
AAGGTGCGTCCGATATCCATATCGAAACGTTTGAAAAGACGCTTTCTATCCGCTTCCGTGTCGACGGCGTTCTGCGTGAA
GTCTTGACGCCGAGTCGTAAGCTTGCGCCGTTATTGGTGTCGCGTGTGAAGGTTATGGCGAAGCTCGATATTGCTGAAAA
GCGTGTGCCTCAAGATGGTCGTATCTCGCTACGTATTGGTGGCCGAGCGGTCGACGTACGTGTTTCTACCATGCCATCCT
CTCACGGTGAACGAGTGGTAATGCGTCTGTTAGATAAAAACGCAACGCGCCTTGACCTGCATAGTTTGGGCATGACTCCT
TCGGTACACGACAACTTCCGCCACCTCATTGGTCGCCCGCACGGAATCATCTTGGTGACCGGCCCTACGGGTTCTGGTAA
ATCGACCACTTTGTACGCCGGCTTGCAAGAAATCAACAGCAACGAGCGCAACATTCTGACGGTGGAAGACCCAATCGAAT
TTGATATCGATGGCATTGGCCAGACCCAAGTTAACCCGAAAGTCGATATGACATTTGCGCGTGGTCTACGTGCGATCTTA
CGTCAGGACCCGGATGTGGTGATGGTTGGTGAGATCCGTGACTTAGAAACCGCGCAAATTGCGGTGCAAGCGTCTTTGAC
TGGTCACTTGGTAATGTCGACGCTGCATACCAACACCGCTGTTGGTGCGATCACGCGTCTGCGTGATATGGGTATTGAAC
CATTTTTGATCTCATCTTCACTGCTTGGTGTGCTAGCGCAGCGTCTGGTTCGTACGCTTTGTTCTGACTGTAAAGAACCT
TACGAAGCGGACAGCGAACAGAAAAAATTGTTTGGCATGGCAGAAAGTGAATCGCTCATTCTACATCGCGCGAAAGGCTG
TGAAGCGTGTAACCAAAAAGGTTATCGCGGACGTACCGGCATTCATGAATTAGTGCTTGTCGATGAAACGGTTCAAGAGC
TGATTCACCGCGAAGCAGGTGAGCAAGAAGTGGAAAAAGCCGTCCGTAATCATACGCCAAGTATTCGCAGTGATGGTCTA
AGCAAAGTACGTCGTGGTTTTACGTCACTTGAAGAAGTGATGCGAGTGACGAAGGAAGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(126-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.939

100

0.45
  pilB Legionella pneumophila strain ERS1305867

43.763

97.8

0.428
  pilF Thermus thermophilus HB27

43.949

94.2

0.414
  pilB Acinetobacter baumannii D1279779

42.62

96.2

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

46.21

81.8

0.378
  pilB Vibrio campbellii strain DS40M4

46.21

81.8

0.378
  pilB Vibrio cholerae strain A1552

47.222

79.2

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.692

78

0.372
  pilB Acinetobacter baylyi ADP1

46.53

77.8

0.362
  pilF Neisseria gonorrhoeae MS11

45.939

78.8

0.362