Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FF090_RS15650 Genome accession   NZ_CP040709
Coordinates   3302603..3304327 (+) Length   574 a.a.
NCBI ID   WP_138857606.1    Uniprot ID   A0A840S462
Organism   Inhella inkyongensis strain IMCC1713     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3297603..3309327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FF090_RS15620 proB 3298188..3299342 (-) 1155 WP_138857602.1 glutamate 5-kinase -
  FF090_RS15625 cgtA 3299348..3300400 (-) 1053 WP_138857603.1 Obg family GTPase CgtA -
  FF090_RS15630 rpmA 3300551..3300808 (-) 258 WP_138857604.1 50S ribosomal protein L27 -
  FF090_RS15635 rplU 3300836..3301147 (-) 312 WP_138857605.1 50S ribosomal protein L21 -
  FF090_RS15640 - 3301423..3302352 (+) 930 WP_138858419.1 polyprenyl synthetase family protein -
  FF090_RS15650 pilB 3302603..3304327 (+) 1725 WP_138857606.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FF090_RS15655 pilC 3304364..3305587 (+) 1224 WP_138857607.1 type II secretion system F family protein Machinery gene
  FF090_RS15660 - 3305588..3306517 (+) 930 WP_138857608.1 A24 family peptidase -
  FF090_RS15665 coaE 3306522..3307118 (+) 597 WP_246071441.1 dephospho-CoA kinase -
  FF090_RS15670 zapD 3307150..3307902 (+) 753 WP_138857610.1 cell division protein ZapD -
  FF090_RS15675 - 3307899..3308093 (+) 195 WP_138857611.1 DNA gyrase inhibitor YacG -
  FF090_RS15680 - 3308080..3308715 (-) 636 WP_175423689.1 HAD family phosphatase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62515.69 Da        Isoelectric Point: 5.7975

>NTDB_id=366716 FF090_RS15650 WP_138857606.1 3302603..3304327(+) (pilB) [Inhella inkyongensis strain IMCC1713]
MQAEPPVQDPHAKLSGAARILVNAGRLQARAAEDLLRQAKEKKLGFAAAAVSSGAISATDLAYTLSQALSIPLLDLNAVD
VQRLPRDILDNKLAAQYQVIALGRRGNRLTIGGADPTEQEVVERIKFATQLQSDWVIVEQDKLSKLLSAAGASASETLES
MSTGDIDFDIAEDATESKDDAQDIAEVEDAPVVRFLQKMLVDAINLRASDLHFEPYEYHYRVRFRVDGELREITQPPIAI
KDKLASRIKVISRMDIAERRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEKVEKERLLN
AVHRPYGMILVTGPTGSGKTVSLYTCLNIMNQPGVNISTVEDPAEINLPGVNQVNVNDKAGLTFAAALKSFLRQDPDVIM
VGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVVPFNIASSVILITAQRLARRLCESCKQPAEYPVEAL
LKAGYKEEELDGSWKPYRAVGCSSCNNGYKGRVGIYQVMPVNEGMASIILAQGTALDIARQAQLDGVRDLRQAGLHKVKL
GVTSLEEVLSATNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=366716 FF090_RS15650 WP_138857606.1 3302603..3304327(+) (pilB) [Inhella inkyongensis strain IMCC1713]
ATGCAAGCCGAGCCGCCTGTTCAAGACCCCCACGCCAAACTGAGCGGTGCCGCGCGCATCTTGGTCAATGCCGGCCGACT
GCAGGCTCGGGCTGCCGAGGACCTGCTGCGACAAGCCAAAGAAAAGAAACTCGGCTTTGCGGCAGCGGCAGTGAGCTCAG
GGGCAATCAGCGCCACAGATTTGGCGTACACCCTGTCGCAGGCACTTTCGATTCCGCTGCTGGATCTCAATGCAGTGGAT
GTCCAACGACTGCCCCGCGACATCCTCGACAACAAGCTCGCAGCTCAGTACCAAGTTATCGCACTGGGTCGTCGCGGCAA
TCGCTTGACGATTGGCGGCGCCGACCCCACCGAACAAGAAGTCGTCGAACGCATCAAGTTCGCCACCCAGTTGCAGTCGG
ACTGGGTGATCGTCGAGCAGGACAAACTTTCCAAGCTCCTTTCGGCAGCGGGCGCCTCGGCCTCCGAAACACTGGAGAGC
ATGTCAACCGGCGACATCGACTTTGATATCGCCGAGGACGCCACCGAAAGCAAGGACGACGCGCAAGACATCGCAGAGGT
CGAAGACGCGCCAGTCGTCCGCTTCCTGCAAAAGATGTTGGTTGACGCCATCAACCTGCGCGCGTCCGATTTGCACTTCG
AACCCTACGAGTACCACTATCGCGTGCGCTTTCGCGTCGACGGGGAGTTGCGCGAAATCACACAGCCCCCTATCGCCATC
AAGGACAAGCTGGCGTCACGCATCAAGGTGATTTCGCGCATGGACATCGCCGAACGCCGAGTCCCGCAAGATGGCCGGAT
GAAGCTGAAGTTTGGTAGCAAGGCCATCGACTTCCGCGTCAGCACCTTGCCCACACTATTTGGCGAGAAGATCGTGATCC
GCATCTTGGATCCGTCCTCCGCCAAGCTGGGCATCGACGCCCTGGGTTATGAAAAGGTGGAGAAGGAACGCCTGCTCAAC
GCGGTTCATCGCCCCTATGGAATGATTTTGGTGACCGGCCCGACAGGCTCGGGCAAGACGGTGTCGCTCTACACCTGTCT
GAACATCATGAATCAGCCGGGCGTGAACATTTCGACCGTAGAGGACCCGGCAGAAATCAACCTGCCTGGCGTCAATCAGG
TCAATGTGAATGACAAGGCCGGGCTGACCTTTGCAGCCGCCCTCAAGTCCTTCCTGCGCCAGGATCCGGACGTGATCATG
GTGGGTGAGATTCGCGACCTCGAAACGGCGGACATTGCCATCAAGGCCGCGCAGACCGGTCACTTGGTGTTGTCCACCCT
GCACACCAATGACGCTCCCACCACCCTGACCCGCCTGATGAACATGGGCGTCGTGCCCTTCAACATCGCATCCAGCGTCA
TCCTGATTACCGCACAGCGACTGGCACGTCGTTTATGCGAAAGCTGCAAACAACCCGCTGAGTATCCGGTGGAGGCACTC
CTGAAAGCCGGCTACAAGGAGGAAGAGCTTGATGGCAGTTGGAAGCCCTACCGCGCTGTGGGTTGTTCGTCCTGCAACAA
CGGCTACAAGGGCCGGGTCGGCATCTACCAAGTGATGCCGGTCAACGAAGGGATGGCCAGCATCATCCTGGCGCAAGGCA
CGGCGCTCGACATTGCCCGCCAAGCACAGCTGGATGGTGTGCGGGATCTGCGCCAGGCCGGCCTGCACAAAGTCAAGCTG
GGCGTCACCTCACTCGAAGAAGTGTTGTCGGCCACCAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-154)

Type II/IV secretion system protein (T2SSE)

(198-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A840S462

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.723

98.258

0.528
  pilF Neisseria gonorrhoeae MS11

53.641

98.084

0.526
  pilB Acinetobacter baumannii D1279779

53.014

98.258

0.521
  pilB Legionella pneumophila strain ERS1305867

49.648

98.955

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

46.82

98.606

0.462
  pilB Vibrio campbellii strain DS40M4

46.359

98.084

0.455
  pilB Vibrio cholerae strain A1552

48.842

90.244

0.441
  pilF Thermus thermophilus HB27

36.632

100

0.368

Multiple sequence alignment