Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FDY98_RS10945 Genome accession   NZ_CP040451
Coordinates   2382429..2384177 (-) Length   582 a.a.
NCBI ID   WP_027959306.1    Uniprot ID   A0A6B8QPR8
Organism   Halomonas sp. PA16-9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2377429..2389177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDY98_RS10915 (FDY98_10970) - 2377907..2378266 (+) 360 WP_035537408.1 hypothetical protein -
  FDY98_RS10920 (FDY98_10975) - 2378290..2379177 (-) 888 WP_022523596.1 hypothetical protein -
  FDY98_RS10925 (FDY98_10980) yacG 2379217..2379450 (-) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  FDY98_RS10930 (FDY98_10985) coaE 2379486..2380075 (-) 590 Protein_2345 dephospho-CoA kinase -
  FDY98_RS10935 (FDY98_10990) pilD 2380155..2381000 (-) 846 WP_027959304.1 A24 family peptidase Machinery gene
  FDY98_RS10940 (FDY98_11000) pilC 2381193..2382425 (-) 1233 WP_027959305.1 type II secretion system F family protein Machinery gene
  FDY98_RS10945 (FDY98_11005) pilB 2382429..2384177 (-) 1749 WP_027959306.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FDY98_RS10950 (FDY98_11010) - 2384419..2384919 (+) 501 WP_196517982.1 pilin -
  FDY98_RS10955 (FDY98_11015) - 2385032..2385466 (+) 435 WP_027959308.1 anti-virulence regulator CigR family protein -
  FDY98_RS10960 (FDY98_11020) - 2385561..2386382 (+) 822 WP_027959309.1 inositol monophosphatase family protein -
  FDY98_RS27795 - 2386735..2386845 (-) 111 WP_309599316.1 IS66 family transposase zinc-finger binding domain-containing protein -
  FDY98_RS10975 (FDY98_11035) - 2386993..2388006 (-) 1014 WP_027959310.1 D-glycerate dehydrogenase -
  FDY98_RS10980 (FDY98_11040) - 2387999..2388928 (-) 930 WP_027959311.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63935.63 Da        Isoelectric Point: 6.9692

>NTDB_id=364579 FDY98_RS10945 WP_027959306.1 2382429..2384177(-) (pilB) [Halomonas sp. PA16-9]
MSQPSLESTLSHASAHGGLRGIAQRLVKHGLLTNAQAATAETTASDLEISLLQHVIESGLVDPNKATLAAGWEYGLPVIN
LEAVRLTSLPPAADYPVKVLQSLNVLPLALHGHRLTVAVPYPATLTRLDELQFATGLSIEGILAPVDQITVVLNSYLAHN
ERGMLDELDDVVSSLSIMQNNGGNDVPLEESSQNSEDAPIVKFVNKILLDAIRRGASDIHFEPYEALYRVRFRVDGMLIE
VARPPFAMRNRIAARLKIMARLDISERRLPQDGSIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTAMQLGIEQLGFT
DEQRRHYEYALKQPQGMILVTGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLDFASALRAF
LRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSATETLTRLANMGVSSFNIASAVSLIVAQRLARKLCTHCKE
PIDIPHEALRKEGFSSEEIHQATIYKPLGCKHCTHGYKGRIGIYEVVPITEAMRQLIMRDANALDIDQQVRKEGYPSLHR
SGLLKVMQGITSLEEVNRVSKE

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=364579 FDY98_RS10945 WP_027959306.1 2382429..2384177(-) (pilB) [Halomonas sp. PA16-9]
ATGAGCCAGCCCTCCTTAGAGTCGACGTTAAGCCACGCCTCCGCACATGGCGGCCTGCGCGGCATTGCCCAGCGCTTGGT
CAAGCACGGCTTGCTGACCAACGCCCAGGCCGCAACAGCGGAAACAACCGCGTCGGACTTGGAAATATCGTTACTTCAGC
ATGTGATCGAAAGCGGCCTGGTCGACCCTAACAAAGCCACACTGGCGGCTGGCTGGGAGTATGGGCTTCCAGTCATTAAC
CTGGAGGCGGTGCGGCTTACCTCACTGCCCCCCGCTGCAGACTATCCTGTCAAAGTACTGCAGAGCCTTAACGTTTTGCC
ATTAGCGCTCCACGGTCATCGCCTCACGGTAGCGGTGCCCTACCCCGCTACATTGACGCGACTGGATGAATTGCAGTTTG
CAACAGGCCTGAGCATTGAAGGCATCCTCGCGCCCGTTGATCAAATCACTGTCGTTCTCAATAGCTACCTTGCTCACAAC
GAGCGCGGCATGCTGGATGAGTTGGACGATGTGGTCAGCTCGCTGAGCATTATGCAGAACAACGGCGGTAACGATGTACC
GCTGGAAGAGTCTTCCCAAAATAGCGAAGATGCACCCATCGTTAAATTTGTGAACAAAATTCTGCTAGATGCTATCCGCC
GAGGCGCCTCCGATATCCATTTCGAACCTTACGAAGCGCTTTACCGAGTACGCTTTCGGGTTGATGGAATGCTCATAGAA
GTGGCACGGCCACCATTCGCCATGCGCAATCGTATCGCGGCCCGGCTAAAAATCATGGCCCGCCTTGATATTTCCGAGCG
CCGCCTGCCTCAGGATGGCTCGATAAAACTCAAGCTTTCGCGCACCCGCTCCCTCGATTTCCGGGTTAATTCTCTACCTA
CTGTCTATGGTGAAAAGCTGGTGCTGCGAATATTAGACCCTACCGCAATGCAGCTGGGTATTGAGCAACTTGGATTTACC
GACGAACAACGGCGCCATTATGAGTACGCCTTAAAACAACCCCAAGGCATGATTCTAGTAACCGGCCCTACCGGTAGTGG
TAAAACCGTTTCGCTGTATACCGGCATTAATATTCTTAATAAAGTGGAACGCAATATTTGCACCGCCGAAGACCCGGTTG
AAATCAAAGTCGCGGGTATCAATCAGGTGAACGTATTGCCTAAGATCGGCCTCGATTTTGCCAGTGCGTTAAGGGCGTTT
CTGCGCCAAGACCCTGATGTGGTCATGGTGGGAGAGATTCGTGACCTTGAAACAGCCGAAATTGCCGTCAAAGCGGCTCA
GACAGGCCACCTGGTACTTTCCACGGTGCATACCAATTCGGCAACTGAGACACTGACTCGACTGGCTAATATGGGAGTCT
CTTCGTTTAATATCGCCAGCGCCGTTAGCTTGATTGTTGCCCAACGGCTGGCTCGCAAACTATGCACACACTGCAAAGAG
CCCATTGACATTCCCCACGAGGCGCTGCGCAAAGAAGGCTTTAGTAGCGAAGAAATTCATCAGGCAACGATCTACAAACC
GTTAGGTTGCAAGCACTGTACTCACGGTTATAAAGGTCGAATTGGCATTTATGAGGTAGTGCCGATCACCGAAGCGATGC
GTCAACTAATTATGCGTGATGCCAACGCCTTAGATATTGATCAACAGGTGCGCAAGGAGGGCTATCCAAGCCTTCACCGC
AGTGGGTTACTGAAAGTCATGCAGGGTATTACCAGCCTGGAAGAGGTTAACCGAGTGAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(206-473)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B8QPR8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

50.796

97.079

0.493
  pilB Legionella pneumophila strain ERS1305867

50.53

97.251

0.491
  pilB Acinetobacter baylyi ADP1

49.736

97.766

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

47.909

98.625

0.473
  pilB Vibrio campbellii strain DS40M4

47.972

97.423

0.467
  pilB Vibrio cholerae strain A1552

47.842

95.533

0.457
  pilF Neisseria gonorrhoeae MS11

49.48

82.646

0.409

Multiple sequence alignment