Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FEO88_RS18185 Genome accession   NZ_CP040439
Coordinates   3907439..3909172 (-) Length   577 a.a.
NCBI ID   WP_154353251.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain PEG-141     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3902439..3914172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEO88_RS18175 (FEO88_18335) - 3903869..3905482 (+) 1614 WP_154353249.1 ATP-binding protein -
  FEO88_RS18180 (FEO88_18340) pilR 3905543..3906916 (+) 1374 WP_154353250.1 sigma-54 dependent transcriptional regulator Regulator
  FEO88_RS18185 (FEO88_18345) pilB 3907439..3909172 (-) 1734 WP_154353251.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FEO88_RS18190 (FEO88_18350) - 3909393..3909800 (-) 408 WP_154353650.1 pilin -
  FEO88_RS18195 (FEO88_18355) - 3909951..3910376 (-) 426 WP_154353252.1 pilin -
  FEO88_RS18200 (FEO88_18360) - 3910729..3911983 (+) 1255 Protein_3584 type II secretion system F family protein -
  FEO88_RS18205 (FEO88_18365) - 3911992..3912855 (+) 864 WP_032128827.1 A24 family peptidase -
  FEO88_RS18210 (FEO88_18370) coaE 3912867..3913478 (+) 612 WP_154264260.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62353.25 Da        Isoelectric Point: 6.1547

>NTDB_id=364442 FEO88_RS18185 WP_154353251.1 3907439..3909172(-) (pilB) [Stenotrophomonas maltophilia strain PEG-141]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGASGSDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FHDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPANLPE
HALLAEGFTQAQLDAGIQLYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=364442 FEO88_RS18185 WP_154353251.1 3907439..3909172(-) (pilB) [Stenotrophomonas maltophilia strain PEG-141]
ATGAATGCCGTAACCACCGCCAACCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGACGGCGCCCTGGATGA
GGCCAGCGCCCGCGACGCCATGGCCAAGGCCGCCGCGGCCCGTCAGCCGTTGCCCACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTTGACGTGTCCACCTTCGATGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGATGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGCCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGACGCCCTGGGCGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCTCCGGCAGCGACTCCGGCATCGACGC
CAAGGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCCATCCGCAAGGGCGCATCGGACATCC
ATTTCGAGCCTTACGAAGACGACTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCAGTG
AAGTTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAGAAGCGCGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTATTCGGTGAAAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCACGACGCCATCCACAAACCGTATGGGATGGTACTGGTGACCGGCCCGACCGGTTCCGGCAAGACGGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGATGAGACGCGCAACATTTCCACTGCCGAAGACCCGGTGGAAATCCGCTTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCTGCGGCGCTGCGCTCGTTCCTGCGACAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCACGCCGGTTGTGCGGTAACTGCAAGCGACCGGCCAACCTGCCGGAG
CACGCGCTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCCAGTTGTACGAGGCGGTGGGCTGCGATGA
GTGCACCGAGGGCTACAAGGGCCGTACTGGTATCTACCAGGTGATGCCGATGACCGATGAGATCGCCACGATCGTGCTGG
CCGGTGGCAACGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAG
GCGGCAGCGGGTGTGACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.214

100

0.56
  pilB Acinetobacter baumannii D1279779

55.888

98.614

0.551
  pilB Legionella pneumophila strain ERS1305867

51.579

98.787

0.51
  pilB Vibrio cholerae strain A1552

48.342

99.307

0.48
  pilF Neisseria gonorrhoeae MS11

48.246

98.787

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.133

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447
  pilF Thermus thermophilus HB27

36.945

97.574

0.36

Multiple sequence alignment