Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ALVIN_RS00820 Genome accession   NC_013851
Coordinates   171133..172848 (-) Length   571 a.a.
NCBI ID   WP_012969408.1    Uniprot ID   D3RVK5
Organism   Allochromatium vinosum DSM 180     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 166133..177848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALVIN_RS00795 (Alvin_0158) glcF 166438..167664 (+) 1227 WP_012969403.1 glycolate oxidase subunit GlcF -
  ALVIN_RS00800 (Alvin_0159) - 167704..168051 (-) 348 WP_012969404.1 hypothetical protein -
  ALVIN_RS00805 (Alvin_0160) coaE 168335..168946 (-) 612 WP_012969405.1 dephospho-CoA kinase -
  ALVIN_RS00810 (Alvin_0161) pilD 168943..169833 (-) 891 WP_012969406.1 A24 family peptidase Machinery gene
  ALVIN_RS00815 (Alvin_0162) pilC 169860..171110 (-) 1251 WP_012969407.1 type II secretion system F family protein Machinery gene
  ALVIN_RS00820 (Alvin_0163) pilB 171133..172848 (-) 1716 WP_012969408.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ALVIN_RS00825 (Alvin_0164) - 172875..173708 (-) 834 WP_012969409.1 NYN domain-containing protein -
  ALVIN_RS00830 (Alvin_0165) acs 173927..175873 (-) 1947 WP_012969410.1 acetate--CoA ligase -
  ALVIN_RS00835 (Alvin_0166) - 176028..176705 (-) 678 WP_012969411.1 exonuclease domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63197.48 Da        Isoelectric Point: 5.6334

>NTDB_id=36258 ALVIN_RS00820 WP_012969408.1 171133..172848(-) (pilB) [Allochromatium vinosum DSM 180]
MSKDQAMLKPSGLAWRLVKDQLISESQMQAAYQEARSKGRTFVQHLVEEKILDSRRIAIVAAQEFGVPLLDLESFDVSNL
PPNLVDERLISKHRALPLLRRGNRLFLALSDPTSDQALEEIRFHTGLATDPILVEDSKLGAAIQKTIQAQDTSTISEFAD
ADLEKLEIEGGEEDDTENLDATIDEAPVVRYVNKILVDAITAGASDIHFEPFEKFFRIRFRQDGLLREVANPPLNIANRL
TSRLKVMSRMNIAERRVPQDGRIKLKLSRNRDIDFRVNTLPTIYGEKIVLRILDSSSAQVGIDALGFEPEQRNNFLKAIK
KPYGMILVTGPTGSGKTVSLYTALNILNDPEINISTVEDPVEIQVPGINQVNLNPKSGLTFAAALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPPFNIASSVLLIMAQRLARRLCMHCREKVNLPRAALLDE
GFTEQEIDAGLTIYGPVGCDKCNKGYRGRVGIFQVMPISEETSRLILEGGNAMQLAEQARREGIADLRASGLRKVKAGVT
SLEEVNRVTTD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=36258 ALVIN_RS00820 WP_012969408.1 171133..172848(-) (pilB) [Allochromatium vinosum DSM 180]
ATGTCGAAGGATCAAGCCATGCTCAAACCCTCCGGACTGGCCTGGCGACTGGTCAAGGACCAGCTCATCTCCGAGTCCCA
GATGCAGGCGGCCTATCAGGAGGCACGCTCCAAGGGGCGGACCTTCGTGCAGCATCTGGTCGAAGAGAAGATCCTCGACA
GCCGCCGCATCGCCATCGTGGCCGCGCAGGAATTCGGCGTCCCCCTGCTCGACCTGGAGTCCTTCGATGTCTCGAATCTG
CCGCCCAACCTCGTCGATGAGCGGCTGATCAGCAAGCATCGCGCGCTGCCGCTGCTGCGCCGCGGCAACCGGCTGTTCCT
GGCGCTCTCGGACCCGACCAGCGATCAGGCACTCGAGGAGATCCGGTTCCACACCGGACTGGCCACAGACCCGATCCTGG
TCGAGGACAGCAAGCTCGGCGCGGCCATCCAGAAGACGATCCAGGCCCAGGACACCAGCACCATCAGCGAGTTCGCCGAC
GCGGATCTGGAAAAGCTCGAAATCGAGGGTGGCGAGGAGGACGATACCGAGAATCTGGACGCCACCATCGACGAAGCGCC
GGTCGTGCGCTATGTCAACAAGATCCTGGTCGATGCCATCACCGCCGGCGCTTCGGACATCCATTTCGAGCCGTTCGAGA
AGTTCTTCCGTATTCGCTTCCGTCAGGACGGTCTGCTGCGCGAGGTCGCCAACCCGCCACTCAATATCGCCAATCGGCTA
ACCTCGCGCCTGAAGGTCATGTCGCGCATGAACATCGCCGAGCGGCGCGTGCCCCAGGACGGACGCATCAAGCTCAAGCT
CAGCCGCAATCGCGACATCGATTTTCGCGTCAATACGCTGCCGACCATCTATGGCGAAAAGATCGTGCTGCGTATCCTGG
ACTCCAGCTCGGCCCAGGTCGGCATCGACGCGCTCGGCTTCGAACCCGAACAGCGCAACAACTTTCTCAAGGCCATCAAG
AAACCCTACGGCATGATCCTGGTGACAGGACCGACCGGCTCGGGCAAGACGGTCTCGCTCTACACCGCGCTCAACATCCT
CAACGACCCGGAGATCAACATCTCCACCGTCGAGGATCCGGTCGAGATCCAGGTGCCCGGCATCAATCAGGTCAACCTCA
ATCCCAAGAGCGGTCTGACCTTCGCCGCCGCGCTGCGTGCCTTTCTGCGTCAGGATCCGGACATCATCATGGTCGGTGAG
ATCCGCGACCTGGAGACGGCCGAGATCGCGGTCAAGGCCGCCCAGACCGGACACCTGGTGCTCTCGACCCTGCACACCAA
CGACGCGCCCCAGACCCTGACCCGTCTGGCCAACATGGGCGTACCGCCATTCAACATCGCCTCCTCGGTGCTGCTGATCA
TGGCTCAGCGTCTGGCGCGCCGGCTGTGCATGCACTGCCGCGAAAAGGTGAATCTGCCGCGCGCGGCCCTGCTGGACGAG
GGCTTCACCGAGCAGGAGATCGACGCGGGGCTGACCATCTATGGTCCGGTCGGGTGCGACAAGTGCAACAAGGGCTATCG
CGGACGTGTCGGTATCTTTCAGGTGATGCCGATCTCGGAGGAAACCTCGCGTCTGATCCTGGAGGGCGGAAACGCCATGC
AACTGGCCGAACAGGCCCGACGCGAGGGGATCGCTGACCTGCGCGCCTCCGGGCTGCGCAAGGTCAAGGCCGGCGTCACC
AGTCTTGAAGAAGTCAATCGGGTCACGACGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3RVK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.383

98.774

0.557
  pilB Acinetobacter baumannii D1279779

55.323

100

0.555
  pilB Acinetobacter baylyi ADP1

55.319

98.774

0.546
  pilB Vibrio cholerae strain A1552

50.802

98.249

0.499
  pilF Neisseria gonorrhoeae MS11

48.677

99.299

0.483
  pilB Vibrio campbellii strain DS40M4

48.227

98.774

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

47.509

98.424

0.468
  pilF Thermus thermophilus HB27

40.468

97.373

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.779

97.548

0.378

Multiple sequence alignment