Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FCM34_RS19620 Genome accession   NZ_CP039832
Coordinates   4193434..4195140 (+) Length   568 a.a.
NCBI ID   WP_143238136.1    Uniprot ID   -
Organism   Aeromonas caviae strain WCW1-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4188434..4200140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCM34_RS19595 (FCM34_19595) ampD 4189337..4189909 (-) 573 WP_039040507.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FCM34_RS19600 (FCM34_19600) - 4190038..4190508 (+) 471 WP_143238134.1 TIGR02281 family clan AA aspartic protease -
  FCM34_RS19605 (FCM34_19605) nadC 4190512..4191375 (+) 864 WP_042864860.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FCM34_RS19610 (FCM34_19610) - 4191860..4192840 (-) 981 WP_221933459.1 IS5-like element IS5 family transposase -
  FCM34_RS19615 (FCM34_19615) - 4192921..4193430 (+) 510 WP_143238135.1 pilus assembly protein TapA -
  FCM34_RS19620 (FCM34_19620) pilB 4193434..4195140 (+) 1707 WP_143238136.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  FCM34_RS19625 (FCM34_19625) pilC 4195268..4196509 (+) 1242 WP_143238137.1 type II secretion system F family protein Machinery gene
  FCM34_RS19630 (FCM34_19630) pilD 4196587..4197462 (+) 876 WP_143238138.1 prepilin peptidase Machinery gene
  FCM34_RS19635 (FCM34_19635) coaE 4197481..4198095 (+) 615 WP_042864857.1 dephospho-CoA kinase -
  FCM34_RS19640 (FCM34_19640) zapD 4198133..4198855 (+) 723 WP_010675825.1 cell division protein ZapD -
  FCM34_RS19645 (FCM34_19645) yacG 4198866..4199060 (+) 195 WP_010675826.1 DNA gyrase inhibitor YacG -
  FCM34_RS19650 (FCM34_19650) mutT 4199118..4199522 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62566.95 Da        Isoelectric Point: 5.8214

>NTDB_id=360683 FCM34_RS19620 WP_143238136.1 4193434..4195140(+) (pilB) [Aeromonas caviae strain WCW1-2]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVLRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=360683 FCM34_RS19620 WP_143238136.1 4193434..4195140(+) (pilB) [Aeromonas caviae strain WCW1-2]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCAGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTTGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGCATCAAGCTCAAGATCTCGCGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGCTCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTCACCGGTCCCACGGGGTCTGGCAAGACGGTCTCACTCTATACCGGCCTCAACATTCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAAG
CAGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAAGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTTACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAGAAGGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.203

99.824

0.551
  pilB Acinetobacter baylyi ADP1

55.24

99.12

0.548
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Vibrio cholerae strain A1552

53.59

100

0.539
  pilB Vibrio campbellii strain DS40M4

50.707

99.648

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.266

99.12

0.498
  pilF Neisseria gonorrhoeae MS11

46.561

99.824

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

38.156

97.359

0.371

Multiple sequence alignment