Detailed information NTG0358786

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: E8M05_RS03260
• Genome accession: NZ_CP039457
• Coordinates: 586270..588501 (+)
• Length: 743 a.a.
• NCBI ID: WP_048791497.1
• Uniprot ID: A0AAW6Y8M8
• Organism: Streptococcus pasteurianus strain WUSP067
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 581270..593501

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E8M05_RS03230 (E8M05_03225)	-	581920..582780 (+)	861	WP_048791495.1	helix-turn-helix transcriptional regulator	-
E8M05_RS03235 (E8M05_03230)	-	582785..583414 (+)	630	WP_048791504.1	ROK family protein	-
E8M05_RS03240 (E8M05_03235)	-	583646..583927 (-)	282	WP_003063819.1	GIY-YIG nuclease family protein	-
E8M05_RS03245 (E8M05_03240)	-	583902..584663 (-)	762	WP_003063822.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
E8M05_RS03250 (E8M05_03245)	-	584771..585517 (+)	747	WP_003063827.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
E8M05_RS03255 (E8M05_03250)	comEA	585612..586277 (+)	666	WP_048791496.1	helix-hairpin-helix domain-containing protein	Machinery gene
E8M05_RS03260 (E8M05_03255)	comEC/celB	586270..588501 (+)	2232	WP_048791497.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
E8M05_RS03265 (E8M05_03260)	holA	588575..589612 (+)	1038	WP_003063837.1	DNA polymerase III subunit delta	-
E8M05_RS03270 (E8M05_03265)	sodA	589701..590309 (+)	609	WP_013851603.1	superoxide dismutase SodA	-
E8M05_RS03275 (E8M05_03270)	-	590458..591183 (+)	726	WP_003063840.1	YebC/PmpR family DNA-binding transcriptional regulator	-
E8M05_RS03280 (E8M05_03275)	queA	591215..592243 (-)	1029	WP_048791498.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
E8M05_RS03285 (E8M05_03280)	-	592419..592919 (+)	501	WP_003063844.1	VIT1/CCC1 transporter family protein	-

Sequence

Protein

• Length: 743 a.a.
• Molecular weight: 83932.27 Da
• Isoelectric Point: 8.2231

>NTDB_id=358786 E8M05_RS03260 WP_048791497.1 586270..588501(+) (comEC/celB) [Streptococcus pasteurianus strain WUSP067]
MIKYFPIKPIQLAFLLILLYYFCFSKVVGCLMFLLLALVFLWQQYGWKAWCQVCLCLTCFAVFFLTKANQTEQAYQAAPD
HLTKIQMIPDTISVNGDLLSFRGKEAGRTYQIFYSLKSEKEQHFFKTLNQTVVLSGDMDLEEATPQRNFGGFDYRTYLKH
EGIYRVVNLSAINNVELVHKTTLWDKLHDVRRKAIVSIQQHFPAPMQHYMTGLLFGYLDKSFDEMSEVYTSLGIIHLFAL
SGMQVGFFVGAFRFFFLRLGLRRDYVDILQIPFSLVYAGLTGFSVSVIRSLIQSAFANLGIKKLDNLALTLFVLFIVMPN
FLLTMGGILSFTYAFILNFINFDDISRYQKIIAESLTISLASLPLLIHFFAVFQPLSVLLTAIFSVAFDTIMLPMLSIIF
ILSPLVKLTCFNGCFVFLETVIKHTKAILGGPIVFGKPNLGILVLLFLMLGVLYDCWRRKKTAIFLVSLVALLFFQIKHP
LENEVTVVDIGQGDSIFVRDVKGNTLLIDVGGKVSFSEKNAWQERLTDSNAERTLIPYLNSRGVGKIDQLVLTHTDTDHM
GDMLEVVKQVKIDEILVSQGSLTKPDFVAKLQAMKTKVRVVTAGDVLPIMGSHLQVLYPSQTGDGGNNDSVVLYGNLLGK
NFLFTGDLEEEGEQELIATYPNLPVDVLKAGHHGSKGSSSSEFLAHISPQIALISAGQNNRYKHPHQETLERFRVQNMTI
YRTDEQGAIRFRGLNHWKIETVR

Nucleotide

• Length: 2232 bp

>NTDB_id=358786 E8M05_RS03260 WP_048791497.1 586270..588501(+) (comEC/celB) [Streptococcus pasteurianus strain WUSP067]
TTGATTAAATATTTTCCCATTAAGCCAATTCAATTGGCTTTTTTGCTCATTTTGCTCTATTATTTTTGCTTTTCAAAAGT
GGTTGGGTGTTTGATGTTTCTCTTGCTAGCCTTGGTGTTTTTATGGCAACAGTATGGTTGGAAGGCTTGGTGTCAAGTTT
GCTTGTGTTTAACCTGTTTTGCGGTATTTTTTCTGACTAAGGCTAATCAGACAGAGCAGGCTTATCAAGCAGCACCAGAT
CATTTAACAAAAATCCAGATGATTCCTGACACGATTTCGGTCAATGGGGATTTGCTGTCCTTTCGAGGTAAGGAAGCTGG
GCGGACTTATCAGATTTTTTATTCCTTGAAATCGGAGAAAGAACAGCATTTTTTTAAAACATTAAATCAAACGGTTGTTT
TATCTGGTGATATGGACTTGGAAGAAGCAACGCCCCAGCGGAATTTTGGTGGTTTTGATTATCGTACTTACCTTAAACAT
GAAGGTATTTATCGTGTGGTAAATTTGTCGGCGATTAACAATGTTGAACTTGTTCATAAGACGACTTTGTGGGATAAGCT
ACATGATGTTCGACGAAAGGCTATTGTTAGTATTCAGCAGCATTTTCCTGCTCCTATGCAACATTATATGACTGGGCTTT
TGTTTGGCTATCTGGATAAATCGTTTGATGAGATGAGTGAGGTTTACACGAGTTTAGGAATTATTCACCTCTTTGCTCTT
TCAGGCATGCAGGTTGGCTTTTTTGTGGGGGCATTCCGCTTTTTCTTTCTACGTCTTGGGTTACGCCGAGATTATGTGGA
CATACTACAAATCCCATTTTCACTCGTCTATGCGGGGCTGACTGGCTTTTCTGTTTCGGTAATTCGGAGTTTAATTCAAT
CTGCTTTTGCAAATCTTGGTATTAAAAAATTAGATAACTTGGCTTTAACTTTGTTTGTTCTTTTTATAGTTATGCCAAAT
TTTCTGCTGACGATGGGAGGCATTCTTTCGTTTACTTACGCTTTTATTCTCAATTTTATTAACTTTGATGACATTAGTCG
CTATCAGAAAATTATTGCGGAAAGTTTGACGATTTCATTAGCCAGTTTGCCCTTGTTAATTCATTTTTTTGCTGTGTTTC
AACCGTTATCTGTCTTATTGACCGCAATTTTTTCTGTGGCATTTGACACTATTATGTTGCCAATGCTATCAATCATTTTT
ATTTTATCGCCCTTGGTTAAACTGACTTGTTTTAATGGTTGTTTTGTTTTCTTGGAAACTGTTATCAAGCACACTAAGGC
AATTTTGGGCGGTCCGATTGTTTTTGGTAAACCAAATCTTGGTATCTTGGTGCTACTATTTTTAATGTTGGGCGTGCTTT
ATGATTGCTGGCGACGAAAGAAAACGGCAATTTTTCTTGTCAGCTTAGTAGCTCTGCTATTTTTTCAAATCAAGCATCCG
CTAGAAAACGAGGTGACGGTGGTTGATATTGGGCAGGGAGATAGTATTTTTGTGCGTGATGTTAAGGGGAATACGCTGCT
AATTGATGTGGGCGGAAAGGTGAGTTTTAGTGAGAAGAACGCGTGGCAGGAGCGGCTGACGGATAGCAATGCGGAGCGAA
CGTTGATTCCTTATCTCAACAGTCGCGGCGTTGGAAAAATTGACCAGTTAGTTCTGACGCACACGGATACTGACCACATG
GGAGATATGCTAGAGGTGGTAAAGCAGGTGAAAATTGATGAAATTCTGGTTAGTCAGGGAAGTTTGACAAAGCCAGATTT
TGTGGCGAAATTACAAGCCATGAAAACCAAGGTGCGCGTGGTAACAGCTGGTGATGTTTTGCCGATTATGGGAAGTCACC
TGCAAGTGCTTTATCCGAGCCAGACTGGTGATGGTGGTAACAATGACTCGGTGGTGCTTTATGGGAATCTTTTGGGTAAG
AATTTCTTGTTTACAGGAGATTTGGAAGAAGAAGGCGAGCAAGAGTTGATAGCAACTTATCCCAATCTGCCGGTTGATGT
CTTAAAAGCAGGGCATCATGGCTCAAAAGGATCATCAAGTTCAGAATTTCTCGCTCACATTTCACCGCAAATTGCTTTGA
TTTCCGCAGGGCAAAATAACCGATACAAACATCCTCACCAAGAAACGCTTGAACGCTTCCGAGTACAAAACATGACCATT
TACCGCACAGACGAGCAAGGCGCTATCCGCTTTAGAGGACTAAATCACTGGAAAATCGAAACAGTGAGGTAG

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (491-697)

• Competence protein (Competence) (213-459)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis NCTC 12261	52.83	99.865	0.528
comEC/celB	Streptococcus mitis SK321	52.426	99.865	0.524
comEC/celB	Streptococcus pneumoniae TIGR4	51.413	100	0.514
comEC/celB	Streptococcus pneumoniae Rx1	50.875	100	0.509
comEC/celB	Streptococcus pneumoniae D39	50.875	100	0.509
comEC/celB	Streptococcus pneumoniae R6	50.875	100	0.509
comEC	Lactococcus lactis subsp. cremoris KW2	46.972	100	0.47