Detailed information NTG0358767

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: E8M05_RS00850
• Genome accession: NZ_CP039457
• Coordinates: 122039..122476 (+)
• Length: 145 a.a.
• NCBI ID: WP_041974065.1
• Uniprot ID: A0AAW6YJ42
• Organism: Streptococcus pasteurianus strain WUSP067
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 117039..127476

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E8M05_RS00820 (E8M05_00815)	-	118759..119121 (+)	363	WP_003062916.1	DUF1033 family protein	-
E8M05_RS00825 (E8M05_00820)	comYA	119192..120133 (+)	942	WP_003062917.1	competence type IV pilus ATPase ComGA	Machinery gene
E8M05_RS00830 (E8M05_00825)	comYB	120057..121100 (+)	1044	WP_003062919.1	competence type IV pilus assembly protein ComGB	Machinery gene
E8M05_RS00835 (E8M05_00830)	comYC	121100..121393 (+)	294	WP_003062921.1	competence type IV pilus major pilin ComGC	Machinery gene
E8M05_RS00840 (E8M05_00835)	comYD	121377..121808 (+)	432	WP_003062922.1	competence type IV pilus minor pilin ComGD	Machinery gene
E8M05_RS00845 (E8M05_00840)	comGE	121762..122055 (+)	294	WP_080543606.1	competence type IV pilus minor pilin ComGE	-
E8M05_RS00850 (E8M05_00845)	comYF	122039..122476 (+)	438	WP_041974065.1	competence type IV pilus minor pilin ComGF	Machinery gene
E8M05_RS00855 (E8M05_00850)	comYG	122448..122780 (+)	333	WP_003062925.1	competence type IV pilus minor pilin ComGG	Machinery gene
E8M05_RS00860 (E8M05_00855)	comYH	122835..123806 (+)	972	WP_003062926.1	class I SAM-dependent methyltransferase	Machinery gene
E8M05_RS00865 (E8M05_00860)	-	123844..125043 (+)	1200	WP_003062927.1	acetate kinase	-
E8M05_RS00870 (E8M05_00865)	-	125204..125404 (+)	201	WP_136596396.1	helix-turn-helix transcriptional regulator	-
E8M05_RS00875 (E8M05_00870)	-	125414..125623 (+)	210	WP_003062929.1	hypothetical protein	-
E8M05_RS00880 (E8M05_00875)	-	125660..126088 (+)	429	WP_230321696.1	ABC transporter permease	-
E8M05_RS00885 (E8M05_00880)	-	126100..126738 (+)	639	WP_003062931.1	CPBP family intramembrane glutamic endopeptidase	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16184.48 Da
• Isoelectric Point: 8.4590

>NTDB_id=358767 E8M05_RS00850 WP_041974065.1 122039..122476(+) (comYF) [Streptococcus pasteurianus strain WUSP067]
MLKKTSLKAFTLIECLVSLLVISGAILVYNGLTQSISANVHYLSENQEENWLLFSQQLRAELANCQLDKVENNKLYVTKS
SQKLAFGQSKADDFRKTNASGQGYQPMIFGVRSSAISRDGQKVTMTLTLENGLERTFVYTFETAS

Nucleotide

• Length: 438 bp

>NTDB_id=358767 E8M05_RS00850 WP_041974065.1 122039..122476(+) (comYF) [Streptococcus pasteurianus strain WUSP067]
ATGTTAAAAAAGACTAGTTTAAAGGCATTCACCCTCATAGAATGTTTGGTTTCTTTATTGGTAATTTCGGGTGCTATTCT
TGTTTACAATGGCTTAACGCAATCTATTTCTGCAAATGTGCATTATTTGTCGGAAAATCAAGAGGAAAACTGGCTTTTGT
TTTCACAACAGCTGCGTGCGGAACTTGCCAATTGTCAATTAGATAAGGTTGAAAATAACAAGCTATATGTGACGAAGTCC
AGTCAAAAGTTGGCATTTGGACAGTCAAAGGCTGATGATTTTCGTAAAACAAACGCATCTGGTCAAGGCTATCAGCCAAT
GATATTTGGAGTAAGATCTTCTGCTATTTCTAGAGATGGTCAGAAGGTGACAATGACATTGACTTTAGAGAATGGTTTGG
AGAGGACATTTGTTTACACTTTTGAAACGGCAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.273	95.862	0.559
comYF	Streptococcus mutans UA159	57.554	95.862	0.552
comGF/cglF	Streptococcus pneumoniae Rx1	45	96.552	0.434
comGF/cglF	Streptococcus pneumoniae D39	45	96.552	0.434
comGF/cglF	Streptococcus pneumoniae R6	45	96.552	0.434
comGF/cglF	Streptococcus pneumoniae TIGR4	45	96.552	0.434
comGF/cglF	Streptococcus mitis NCTC 12261	43.662	97.931	0.428
comGF/cglF	Streptococcus mitis SK321	42.958	97.931	0.421
comGF	Lactococcus lactis subsp. cremoris KW2	43.478	95.172	0.414