Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   E8M05_RS00830 Genome accession   NZ_CP039457
Coordinates   120057..121100 (+) Length   347 a.a.
NCBI ID   WP_003062919.1    Uniprot ID   E0PB48
Organism   Streptococcus pasteurianus strain WUSP067     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115057..126100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M05_RS00820 (E8M05_00815) - 118759..119121 (+) 363 WP_003062916.1 DUF1033 family protein -
  E8M05_RS00825 (E8M05_00820) comYA 119192..120133 (+) 942 WP_003062917.1 competence type IV pilus ATPase ComGA Machinery gene
  E8M05_RS00830 (E8M05_00825) comYB 120057..121100 (+) 1044 WP_003062919.1 competence type IV pilus assembly protein ComGB Machinery gene
  E8M05_RS00835 (E8M05_00830) comYC 121100..121393 (+) 294 WP_003062921.1 competence type IV pilus major pilin ComGC Machinery gene
  E8M05_RS00840 (E8M05_00835) comYD 121377..121808 (+) 432 WP_003062922.1 competence type IV pilus minor pilin ComGD Machinery gene
  E8M05_RS00845 (E8M05_00840) comGE 121762..122055 (+) 294 WP_080543606.1 competence type IV pilus minor pilin ComGE -
  E8M05_RS00850 (E8M05_00845) comYF 122039..122476 (+) 438 WP_041974065.1 competence type IV pilus minor pilin ComGF Machinery gene
  E8M05_RS00855 (E8M05_00850) comYG 122448..122780 (+) 333 WP_003062925.1 competence type IV pilus minor pilin ComGG Machinery gene
  E8M05_RS00860 (E8M05_00855) comYH 122835..123806 (+) 972 WP_003062926.1 class I SAM-dependent methyltransferase Machinery gene
  E8M05_RS00865 (E8M05_00860) - 123844..125043 (+) 1200 WP_003062927.1 acetate kinase -
  E8M05_RS00870 (E8M05_00865) - 125204..125404 (+) 201 WP_136596396.1 helix-turn-helix transcriptional regulator -
  E8M05_RS00875 (E8M05_00870) - 125414..125623 (+) 210 WP_003062929.1 hypothetical protein -
  E8M05_RS00880 (E8M05_00875) - 125660..126088 (+) 429 WP_230321696.1 ABC transporter permease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39817.38 Da        Isoelectric Point: 9.8728

>NTDB_id=358764 E8M05_RS00830 WP_003062919.1 120057..121100(+) (comYB) [Streptococcus pasteurianus strain WUSP067]
MQKLKVLLKTDISQLNKQKSKKLPFKKQRKVIQLFNNLFKSGFNLTEIVFFLRRSQLLSEVYVERMQESLLNGASLAAMM
VDLGFSDNIVTQIALADVHGNSQKSLLKIESYLSSMTVVRKKLIEVATYPLILFLFLILIMLGLKNYLLPQLESQNVATQ
IIAHFPTIFLLSIFSIGVLLICTTFYARRLSQIDLYSRISRIPLVGNYVRLYLTAYYAREWGNLIGQGIELMAIVGIMQK
QKSLLFQEIGKDMEEALLSGQAFHQKVLDYPFFLRELSLMIEYGEIKSKLGRELDIYAEETWQSFFGKLTQATQLIQPLV
FVFVALIIVLIYVAMLLPMYQNMGGNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=358764 E8M05_RS00830 WP_003062919.1 120057..121100(+) (comYB) [Streptococcus pasteurianus strain WUSP067]
ATGCAAAAATTGAAAGTCTTGTTAAAGACGGACATATCACAGCTGAACAAGCAAAAATCGAAAAAATTGCCATTTAAAAA
ACAGCGCAAGGTTATCCAACTCTTTAATAATCTTTTTAAAAGTGGGTTTAATTTAACAGAAATTGTGTTTTTTCTCCGAA
GAAGTCAATTGTTGTCAGAGGTTTATGTTGAGAGGATGCAAGAAAGTTTGTTAAATGGTGCTAGTCTAGCAGCAATGATG
GTAGATTTAGGGTTTTCGGACAATATTGTCACACAAATTGCTTTGGCTGATGTTCATGGAAACAGTCAGAAAAGTCTGCT
AAAAATTGAGTCTTACCTTTCTAGCATGACTGTCGTCAGAAAAAAGTTAATTGAAGTTGCAACGTATCCATTGATTTTGT
TCTTGTTTCTTATTTTGATTATGCTGGGGTTGAAGAATTATTTACTGCCGCAGCTGGAAAGCCAAAATGTGGCAACGCAG
ATTATTGCGCATTTTCCAACGATTTTTTTGTTAAGTATTTTCTCGATTGGAGTGTTGCTTATTTGTACGACATTTTATGC
TAGGCGTTTATCGCAGATTGATTTATATAGTCGAATAAGCCGGATTCCACTTGTGGGAAACTATGTTAGGTTATATTTGA
CAGCTTACTATGCGCGTGAATGGGGGAATTTGATTGGGCAAGGTATTGAATTAATGGCAATCGTGGGAATCATGCAAAAG
CAAAAGTCGCTCTTATTTCAAGAGATTGGAAAGGATATGGAAGAAGCGCTGCTTTCAGGGCAAGCTTTTCATCAAAAAGT
TCTGGATTATCCATTCTTTTTGCGAGAATTGAGCTTGATGATTGAATATGGTGAGATCAAATCAAAGCTTGGGCGTGAGT
TGGACATTTATGCTGAGGAAACATGGCAGAGCTTTTTTGGCAAATTGACTCAAGCAACACAGCTCATTCAACCACTTGTT
TTTGTCTTTGTAGCTTTGATTATTGTGTTAATTTATGTGGCAATGCTGTTGCCAATGTATCAAAATATGGGAGGAAATTT
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0PB48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

66.456

91.066

0.605
  comYB Streptococcus mutans UA159

66.139

91.066

0.602
  comGB/cglB Streptococcus mitis NCTC 12261

59.587

97.695

0.582
  comYB Streptococcus gordonii str. Challis substr. CH1

59.524

96.83

0.576
  comGB/cglB Streptococcus mitis SK321

56.677

97.118

0.55
  comGB/cglB Streptococcus pneumoniae Rx1

55.786

97.118

0.542
  comGB/cglB Streptococcus pneumoniae D39

55.786

97.118

0.542
  comGB/cglB Streptococcus pneumoniae R6

55.786

97.118

0.542
  comGB/cglB Streptococcus pneumoniae TIGR4

55.786

97.118

0.542
  comGB Lactococcus lactis subsp. cremoris KW2

50

98.559

0.493

Multiple sequence alignment