Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E8A47_RS10600 Genome accession   NZ_CP039374
Coordinates   2375397..2377145 (-) Length   582 a.a.
NCBI ID   WP_089690942.1    Uniprot ID   -
Organism   Vreelandella titanicae strain ANRCS81     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2370397..2382145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8A47_RS10570 - 2370873..2371232 (+) 360 WP_035537408.1 hypothetical protein -
  E8A47_RS10575 - 2371256..2372143 (-) 888 WP_022523596.1 hypothetical protein -
  E8A47_RS10580 yacG 2372183..2372416 (-) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  E8A47_RS10585 coaE 2372452..2373054 (-) 603 WP_141441161.1 dephospho-CoA kinase -
  E8A47_RS10590 pilD 2373123..2373968 (-) 846 WP_027959304.1 A24 family peptidase Machinery gene
  E8A47_RS10595 pilC 2374161..2375393 (-) 1233 WP_141441160.1 type II secretion system F family protein Machinery gene
  E8A47_RS10600 pilB 2375397..2377145 (-) 1749 WP_089690942.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E8A47_RS10605 - 2377387..2377902 (+) 516 WP_022523590.1 pilin -
  E8A47_RS10610 - 2378011..2378445 (+) 435 WP_141441159.1 anti-virulence regulator CigR family protein -
  E8A47_RS10615 - 2378540..2379361 (+) 822 WP_141441158.1 inositol monophosphatase family protein -
  E8A47_RS24570 - 2379713..2379823 (-) 111 WP_309599316.1 IS66 family transposase zinc-finger binding domain-containing protein -
  E8A47_RS10630 - 2379971..2380984 (-) 1014 WP_027959310.1 D-glycerate dehydrogenase -
  E8A47_RS10635 - 2380977..2381906 (-) 930 Protein_2100 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63990.67 Da        Isoelectric Point: 6.9693

>NTDB_id=358256 E8A47_RS10600 WP_089690942.1 2375397..2377145(-) (pilB) [Vreelandella titanicae strain ANRCS81]
MSQPSLESTLSHASAHGDLRGIAQRLVKHGLLTNAQAATAETTASDLEISLLQHVIESGLVDPNKATLAAGWEYGLPVIN
LEAVRLTSLPPAADYPVKVLQSLNVLPLARHGHRLTVAVPYPATLTRLDELQFATGLSIEGILAPVDQITVVLNSYLAHN
ERGMLDELDDVVSSLSIMQNNGGNDVPLEEASQNSEDAPIVKFVNKILLDAIRRGASDIHFEPYEALYRVRFRVDGMLIE
VARPPFAMRNRIAARLKIMARLDISERRLPQDGSIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTAMQLGIEQLGFT
DEQRRHYEYALKQPQGMILVTGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLDFASALRAF
LRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVSSFNIASAVSLIVAQRLARKLCTHCKE
PIDIPHEALRKEGFSSEEIHQATIYKPLGCKHCTHGYKGRIGIYEVVPITEAMRQLIMRDANALDIDQQVRKEGYPSLHR
SGLLKVMQGITSLEEVNRVSKE

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=358256 E8A47_RS10600 WP_089690942.1 2375397..2377145(-) (pilB) [Vreelandella titanicae strain ANRCS81]
ATGAGCCAGCCTTCCTTAGAGTCGACTTTAAGCCACGCCTCCGCACATGGCGACCTGCGCGGCATTGCCCAGCGCTTGGT
CAAGCACGGCTTGCTGACCAACGCCCAGGCCGCAACAGCGGAAACAACCGCGTCGGACTTGGAAATATCGTTACTTCAGC
ATGTGATCGAAAGCGGCCTGGTCGACCCTAACAAAGCCACACTGGCGGCTGGCTGGGAGTATGGGCTTCCAGTCATTAAC
TTGGAGGCGGTGCGGCTTACCTCACTGCCCCCCGCTGCAGACTATCCTGTCAAAGTACTGCAGAGCCTTAACGTTTTGCC
ATTAGCGCGCCACGGTCATCGCCTCACGGTGGCGGTGCCCTACCCCGCTACATTGACGCGACTGGATGAATTGCAGTTTG
CAACAGGCCTGAGCATTGAAGGTATCCTCGCGCCCGTTGATCAAATCACTGTCGTTCTCAATAGCTACCTTGCTCACAAC
GAGCGCGGCATGCTGGATGAGTTGGACGATGTGGTCAGCTCGCTGAGCATTATGCAGAACAACGGCGGTAACGATGTACC
GCTGGAAGAGGCTTCCCAAAATAGCGAAGATGCACCCATCGTTAAATTTGTGAACAAAATTCTGCTAGATGCTATCCGCC
GAGGCGCCTCCGATATCCATTTCGAACCTTACGAAGCGCTTTACCGAGTACGCTTTCGGGTTGATGGAATGCTCATAGAA
GTGGCACGGCCACCATTCGCCATGCGCAATCGTATCGCGGCCCGGCTAAAAATCATGGCCCGCCTTGATATTTCCGAGCG
CCGCCTGCCTCAGGATGGCTCGATAAAACTCAAGCTTTCGCGCACCCGCTCCCTCGATTTCCGGGTTAATTCGCTACCTA
CTGTCTATGGTGAAAAGTTGGTGCTGCGAATATTAGACCCTACCGCAATGCAGCTGGGTATTGAGCAACTTGGATTTACC
GACGAACAACGGCGCCATTATGAATACGCCTTAAAACAACCCCAAGGCATGATTCTAGTAACCGGCCCTACCGGTAGTGG
TAAAACCGTTTCGCTGTATACCGGCATTAATATTCTTAATAAAGTGGAACGCAATATTTGCACCGCCGAAGACCCGGTTG
AAATCAAAGTCGCGGGTATCAATCAGGTGAACGTATTGCCTAAGATCGGCCTCGATTTTGCCAGTGCGTTAAGGGCGTTT
CTGCGCCAAGACCCTGATGTGGTCATGGTGGGCGAGATTCGTGACCTTGAAACAGCCGAAATTGCCGTCAAAGCGGCTCA
GACAGGCCACCTGGTACTTTCCACGGTGCATACCAACTCGGCAGCTGAGACACTGACTCGACTGGCTAATATGGGAGTCT
CTTCGTTTAATATCGCCAGCGCCGTTAGCTTGATTGTTGCCCAACGGCTGGCTCGTAAACTATGCACACACTGCAAAGAG
CCCATTGACATTCCCCACGAGGCGTTGCGCAAAGAAGGCTTTAGTAGCGAAGAAATCCATCAGGCAACGATCTACAAACC
GTTAGGTTGCAAGCACTGTACTCACGGTTATAAAGGTCGAATTGGCATTTATGAGGTAGTGCCGATCACCGAAGCGATGC
GTCAACTAATTATGCGTGATGCCAACGCCTTAGATATTGATCAACAGGTGCGCAAGGAGGGCTATCCAAGCCTTCACCGC
AGTGGGTTACTGAAAGTCATGCAGGGTATTACCAGCCTGGAAGAGGTTAACCGAGTGAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-160)

Type II/IV secretion system protein (T2SSE)

(206-473)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.241

96.907

0.497
  pilB Legionella pneumophila strain ERS1305867

50.883

97.251

0.495
  pilB Acinetobacter baylyi ADP1

49.912

97.766

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

47.945

100

0.481
  pilB Vibrio campbellii strain DS40M4

48.325

97.423

0.471
  pilB Vibrio cholerae strain A1552

48.007

94.845

0.455
  pilF Neisseria gonorrhoeae MS11

49.688

82.646

0.411

Multiple sequence alignment