Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   E8A47_RS10590 Genome accession   NZ_CP039374
Coordinates   2373123..2373968 (-) Length   281 a.a.
NCBI ID   WP_027959304.1    Uniprot ID   A0A6B8QQH4
Organism   Vreelandella titanicae strain ANRCS81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2368123..2378968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8A47_RS10560 - 2369204..2369578 (+) 375 WP_022523599.1 histone-like nucleoid-structuring protein, MvaT/MvaU family -
  E8A47_RS10565 - 2369667..2370497 (-) 831 WP_022523598.1 helix-turn-helix transcriptional regulator -
  E8A47_RS10570 - 2370873..2371232 (+) 360 WP_035537408.1 hypothetical protein -
  E8A47_RS10575 - 2371256..2372143 (-) 888 WP_022523596.1 hypothetical protein -
  E8A47_RS10580 yacG 2372183..2372416 (-) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  E8A47_RS10585 coaE 2372452..2373054 (-) 603 WP_141441161.1 dephospho-CoA kinase -
  E8A47_RS10590 pilD 2373123..2373968 (-) 846 WP_027959304.1 A24 family peptidase Machinery gene
  E8A47_RS10595 pilC 2374161..2375393 (-) 1233 WP_141441160.1 type II secretion system F family protein Machinery gene
  E8A47_RS10600 pilB 2375397..2377145 (-) 1749 WP_089690942.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E8A47_RS10605 - 2377387..2377902 (+) 516 WP_022523590.1 pilin -
  E8A47_RS10610 - 2378011..2378445 (+) 435 WP_141441159.1 anti-virulence regulator CigR family protein -

Sequence

Protein


Download         Length: 281 a.a.        Molecular weight: 30801.12 Da        Isoelectric Point: 7.2769

>NTDB_id=358254 E8A47_RS10590 WP_027959304.1 2373123..2373968(-) (pilD) [Vreelandella titanicae strain ANRCS81]
MHHPPFILWLIVLLIGLCLGSFLNVVITRLPVMLMRGWRNEAREALELSAEHSPRFNLVTPGSMCPRCEAPIAWHDNLPL
IGWIKRRGRCAECQTPISVQYPLVEVAGGLLAMAVLALHGLTAESAFVYGACLMLLVLAVIDFRTQLLPDIITLPLLWAG
LLFQLLFQPLMLPSAVIGAMVGYLSLWSFYWLFKLATGKEGMGYGDFKLLAALGAWLGWSFLPLILILSAGLGAIVGLVA
QAYSPNLRGTPFPFGPFLALAGWVALLVGDEIMAMYLNLLM

Nucleotide


Download         Length: 846 bp        

>NTDB_id=358254 E8A47_RS10590 WP_027959304.1 2373123..2373968(-) (pilD) [Vreelandella titanicae strain ANRCS81]
ATGCATCACCCTCCGTTTATCCTGTGGTTAATCGTTTTATTGATTGGGCTATGCCTGGGCAGTTTTCTAAATGTGGTGAT
TACGCGCCTGCCGGTGATGCTGATGCGTGGCTGGCGTAATGAAGCCCGCGAAGCACTTGAGCTTAGCGCTGAACACTCAC
CACGCTTTAATTTGGTCACACCAGGCTCTATGTGTCCACGCTGTGAAGCCCCCATTGCATGGCACGACAACCTGCCACTG
ATTGGCTGGATAAAGCGCCGCGGCCGCTGCGCTGAGTGCCAAACGCCGATTAGCGTTCAATATCCTCTGGTAGAAGTAGC
CGGTGGCTTACTGGCCATGGCGGTGCTAGCGCTCCACGGGCTTACGGCAGAATCAGCCTTCGTCTACGGGGCCTGCCTGA
TGCTGTTAGTGCTCGCCGTGATCGACTTTCGCACCCAGCTTCTCCCCGACATCATCACCCTCCCCTTGCTTTGGGCAGGG
TTACTGTTTCAGCTCCTCTTCCAGCCACTGATGCTGCCAAGTGCCGTTATCGGCGCAATGGTAGGCTATCTTTCACTGTG
GAGCTTTTATTGGCTATTCAAACTGGCCACCGGCAAAGAGGGTATGGGCTATGGCGACTTTAAGCTGCTGGCAGCGCTAG
GCGCCTGGCTGGGTTGGAGTTTTTTACCGCTAATACTGATACTTTCCGCAGGGCTTGGGGCCATTGTTGGGCTAGTTGCC
CAGGCCTACTCACCTAACCTACGCGGCACACCGTTTCCCTTCGGCCCTTTTCTCGCCCTCGCCGGCTGGGTGGCGTTGTT
AGTCGGTGATGAAATAATGGCAATGTATCTCAACCTGCTTATGTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(132-238)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(14-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B8QQH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

49.286

99.644

0.491
  pilD Acinetobacter baumannii D1279779

47.273

97.865

0.463
  pilD Acinetobacter nosocomialis M2

47.273

97.865

0.463
  pilD Vibrio campbellii strain DS40M4

42.958

100

0.434
  pilD Neisseria gonorrhoeae MS11

45.211

92.883

0.42

Multiple sequence alignment