Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GSF12_RS08605 Genome accession   NZ_CP047226
Coordinates   1901698..1903203 (-) Length   501 a.a.
NCBI ID   WP_201450384.1    Uniprot ID   -
Organism   Moraxella osloensis strain YV1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1896698..1908203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF12_RS08590 (GSF12_08590) - 1898375..1899136 (+) 762 WP_159375147.1 class I SAM-dependent methyltransferase -
  GSF12_RS08595 (GSF12_08595) miaB 1899210..1900676 (+) 1467 WP_159375148.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  GSF12_RS08600 (GSF12_08600) - 1900732..1901688 (-) 957 WP_159375149.1 hypothetical protein -
  GSF12_RS08605 (GSF12_08605) pilB 1901698..1903203 (-) 1506 WP_201450384.1 type II secretion system ATPase GspE Machinery gene
  GSF12_RS08610 (GSF12_08610) - 1903377..1904228 (+) 852 WP_228274235.1 thiazole synthase -
  GSF12_RS08615 (GSF12_08615) - 1904256..1905614 (+) 1359 WP_159375150.1 WS/DGAT/MGAT family O-acyltransferase -
  GSF12_RS08620 (GSF12_08620) - 1905687..1906415 (-) 729 WP_159375742.1 amino acid ABC transporter ATP-binding protein -
  GSF12_RS08625 (GSF12_08625) - 1906531..1907202 (-) 672 WP_050324714.1 ABC transporter permease subunit -
  GSF12_RS08630 (GSF12_08630) - 1907199..1907945 (-) 747 WP_159375151.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55420.56 Da        Isoelectric Point: 5.3863

>NTDB_id=358184 GSF12_RS08605 WP_201450384.1 1901698..1903203(-) (pilB) [Moraxella osloensis strain YV1]
MLNPIALPYSFAKRHQLLLQYSQDLTDPPTLLMTADTPMEAINEANRFASGAGVPLPLNFAMAQSDDFEKQLAAVYAGNT
GESQHIAAGLEDHPDLMSLAESVPETEDLMDQEDDAPIIRLINALLSEAIRLNASDIHIETFEKRLSVRFRVDGILKEIV
SPKRELSPLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSNFGERVVMRLLDKQAGRLNMTYLGLSDND
YSELKRLIHRPHGIILVTGPTGSGKTTTLYAALTDLNDNTRNILTAEDPIEFQLDGIGQTQVNNKVDMTFARSLRAMLRQ
DPDVVMVGEVRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLQDMGVEPFLLSSSLIGVVAQRLVRTLCPHCHTWTL
ADAYQTQIFTEIGEIGEIKLPKPVGCEKCNQSGFRGRTAIYEVVPVDDKLRQLIHSQTAEFELEAYARSKTPSIRADGLK
KVLTGKTTLEEVLRVTKEKEI

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=358184 GSF12_RS08605 WP_201450384.1 1901698..1903203(-) (pilB) [Moraxella osloensis strain YV1]
ATGCTAAATCCGATTGCCTTGCCCTATAGTTTTGCCAAACGTCATCAACTGCTGTTGCAATACTCGCAAGATTTGACTGA
CCCACCGACGTTGTTGATGACCGCTGATACCCCTATGGAAGCCATCAACGAAGCCAACCGTTTTGCCAGTGGAGCAGGGG
TGCCGCTGCCATTGAATTTTGCCATGGCACAAAGTGACGATTTTGAAAAGCAGTTAGCCGCGGTCTATGCAGGCAACACA
GGGGAATCTCAGCATATTGCCGCTGGGCTTGAAGATCATCCCGATTTGATGAGTCTCGCTGAAAGCGTCCCTGAGACGGA
AGACTTGATGGACCAAGAAGATGACGCGCCCATCATTCGGCTAATCAACGCGCTACTGTCCGAAGCCATCCGCCTAAACG
CCTCAGATATTCACATCGAAACCTTTGAAAAACGGCTATCGGTGCGCTTTCGGGTAGATGGGATACTCAAAGAAATCGTC
AGTCCAAAACGGGAACTGTCACCGCTACTGGTGTCGCGTATCAAGGTGATGGCAAAACTCGATATCGCTGAAAAACGGGT
ACCGCAAGATGGACGCATCTCACTACGCCTGGCTGGGCGAGAAGTGGATGTGCGGGTATCGACCTTGCCATCAAATTTTG
GTGAGCGTGTGGTGATGCGGCTTTTGGATAAACAAGCGGGTCGGTTAAATATGACCTATTTGGGGTTGTCAGACAATGAT
TATAGCGAGCTTAAACGCTTAATCCATCGCCCGCATGGCATTATTCTTGTCACAGGTCCGACAGGTTCAGGCAAAACCAC
GACGCTGTACGCGGCTTTAACGGATTTGAACGATAACACCCGTAATATTTTGACCGCTGAAGACCCGATTGAATTTCAGC
TAGATGGTATCGGGCAAACCCAAGTCAATAATAAAGTGGATATGACCTTTGCCCGTAGCCTGCGAGCCATGCTACGCCAA
GACCCTGATGTGGTGATGGTGGGGGAGGTTCGCGATTTAGAGACCGCAGAAATTGCGGTGCAAGCGTCACTCACCGGTCA
CTTGGTACTCTCGACCCTGCATACCAACACCGCGATTGGTGCGGTGACGCGACTACAAGATATGGGGGTTGAGCCGTTTT
TGTTGTCATCGAGCCTCATTGGCGTGGTAGCACAGCGACTCGTGCGCACCTTGTGTCCGCATTGCCATACATGGACACTT
GCCGATGCCTACCAAACGCAGATTTTTACTGAAATAGGCGAAATCGGGGAGATTAAACTGCCAAAACCTGTGGGCTGCGA
GAAGTGCAATCAATCAGGATTTCGAGGACGTACTGCGATTTATGAAGTGGTGCCTGTCGATGACAAACTGCGACAACTGA
TTCATAGCCAAACGGCGGAGTTTGAGTTAGAAGCGTATGCGCGGAGTAAGACACCGTCCATTCGTGCCGATGGACTCAAA
AAAGTATTGACGGGTAAAACCACGCTTGAAGAAGTGCTACGGGTGACAAAAGAAAAAGAAATTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(124-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.912

100

0.439
  pilB Vibrio parahaemolyticus RIMD 2210633

44.606

96.208

0.429
  pilB Vibrio campbellii strain DS40M4

44.259

95.609

0.423
  pilB/pilB1 Synechocystis sp. PCC 6803

39.81

100

0.417
  pilB Legionella pneumophila strain ERS1305867

46.588

84.83

0.395
  pilB Acinetobacter baumannii D1279779

46.517

80.24

0.373
  pilF Thermus thermophilus HB27

45.792

80.639

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.044

77.645

0.365
  pilF Neisseria gonorrhoeae MS11

46.907

77.445

0.363
  pilB Acinetobacter baylyi ADP1

45.477

79.441

0.361