Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E7Z57_RS17295 Genome accession   NZ_CP039339
Coordinates   3622554..3623057 (+) Length   167 a.a.
NCBI ID   WP_138929647.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain UW386     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3617554..3628057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E7Z57_RS17270 (E7Z57_17270) - 3617894..3618520 (+) 627 WP_138929644.1 DUF2889 domain-containing protein -
  E7Z57_RS17275 (E7Z57_17275) sucC 3618587..3619753 (+) 1167 WP_064050228.1 ADP-forming succinate--CoA ligase subunit beta -
  E7Z57_RS17280 (E7Z57_17280) sucD 3619839..3620720 (+) 882 WP_064050227.1 succinate--CoA ligase subunit alpha -
  E7Z57_RS17285 (E7Z57_17285) - 3620870..3621568 (+) 699 WP_138929645.1 TerC family protein -
  E7Z57_RS17290 (E7Z57_17290) pilA 3621762..3622283 (+) 522 WP_138929646.1 pilin Machinery gene
  E7Z57_RS17295 (E7Z57_17295) pilA 3622554..3623057 (+) 504 WP_138929647.1 pilin Machinery gene
  E7Z57_RS17300 (E7Z57_17300) - 3623131..3624867 (+) 1737 WP_138929648.1 PglL family O-oligosaccharyltransferase -
  E7Z57_RS17305 (E7Z57_17305) moaC 3624942..3625418 (-) 477 WP_138929649.1 cyclic pyranopterin monophosphate synthase MoaC -
  E7Z57_RS17310 (E7Z57_17310) - 3625512..3627206 (+) 1695 WP_138929650.1 tetratricopeptide repeat protein -
  E7Z57_RS17315 (E7Z57_17315) - 3627221..3627826 (-) 606 WP_138929651.1 DUF2946 family protein -

Sequence

Protein


Download         Length: 167 a.a.        Molecular weight: 17130.83 Da        Isoelectric Point: 9.9252

>NTDB_id=358121 E7Z57_RS17295 WP_138929647.1 3622554..3623057(+) (pilA) [Ralstonia pseudosolanacearum strain UW386]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAANAATDLSAGSSTFA
ATKNVASLAIAQANGEIVIGYTTAVGTGTPKLVLTPTSNGIALVTGSAPTASIMWTCYSKNKATAASSVAPTTTPTLEAK
YAPAECR

Nucleotide


Download         Length: 504 bp        

>NTDB_id=358121 E7Z57_RS17295 WP_138929647.1 3622554..3623057(+) (pilA) [Ralstonia pseudosolanacearum strain UW386]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCTTATCAGGACTACACGATCCGTGCACGTGTGACGGAAGGTTTGTCGCTGG
CTGCTCAAGCCAAGTCGCTCGTAGCTGAGAACGCTGCGAATGCCGCGACTGACCTTTCGGCAGGTTCGTCGACGTTTGCT
GCTACCAAGAACGTTGCGTCTCTCGCTATTGCGCAAGCCAACGGTGAAATCGTTATCGGCTACACGACGGCTGTTGGTAC
TGGAACGCCGAAACTGGTGCTGACGCCTACTTCTAACGGGATTGCCCTGGTGACCGGCAGTGCGCCCACGGCCTCCATTA
TGTGGACCTGCTATTCGAAGAATAAGGCAACGGCTGCTAGTTCGGTTGCTCCGACGACTACCCCGACGCTGGAAGCCAAG
TATGCCCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(44-167)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

74.118

100

0.754
  pilA2 Legionella pneumophila str. Paris

54.938

97.006

0.533
  pilA2 Legionella pneumophila strain ERS1305867

53.704

97.006

0.521
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.895

100

0.431
  comP Acinetobacter baylyi ADP1

41.765

100

0.425

Multiple sequence alignment