Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E7Z57_RS17290 Genome accession   NZ_CP039339
Coordinates   3621762..3622283 (+) Length   173 a.a.
NCBI ID   WP_138929646.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain UW386     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3616762..3627283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E7Z57_RS17265 (E7Z57_17265) recX 3617140..3617619 (+) 480 WP_138929643.1 recombination regulator RecX -
  E7Z57_RS17270 (E7Z57_17270) - 3617894..3618520 (+) 627 WP_138929644.1 DUF2889 domain-containing protein -
  E7Z57_RS17275 (E7Z57_17275) sucC 3618587..3619753 (+) 1167 WP_064050228.1 ADP-forming succinate--CoA ligase subunit beta -
  E7Z57_RS17280 (E7Z57_17280) sucD 3619839..3620720 (+) 882 WP_064050227.1 succinate--CoA ligase subunit alpha -
  E7Z57_RS17285 (E7Z57_17285) - 3620870..3621568 (+) 699 WP_138929645.1 TerC family protein -
  E7Z57_RS17290 (E7Z57_17290) pilA 3621762..3622283 (+) 522 WP_138929646.1 pilin Machinery gene
  E7Z57_RS17295 (E7Z57_17295) pilA 3622554..3623057 (+) 504 WP_138929647.1 pilin Machinery gene
  E7Z57_RS17300 (E7Z57_17300) - 3623131..3624867 (+) 1737 WP_138929648.1 PglL family O-oligosaccharyltransferase -
  E7Z57_RS17305 (E7Z57_17305) moaC 3624942..3625418 (-) 477 WP_138929649.1 cyclic pyranopterin monophosphate synthase MoaC -
  E7Z57_RS17310 (E7Z57_17310) - 3625512..3627206 (+) 1695 WP_138929650.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17741.33 Da        Isoelectric Point: 10.0319

>NTDB_id=358120 E7Z57_RS17290 WP_138929646.1 3621762..3622283(+) (pilA) [Ralstonia pseudosolanacearum strain UW386]
MASKHSRNRWRSQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRSRVTEGLSLAAQAKALVVENAANAQSSLSVGSASL
SASRNVSALNIDSSTGEITVVYASAVTGAGANTLVLTPYSGSSSSLANLQAGSAPPAMVLWVCAAAGKSMPVSTVSQKTT
ATLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=358120 E7Z57_RS17290 WP_138929646.1 3621762..3622283(+) (pilA) [Ralstonia pseudosolanacearum strain UW386]
ATGGCATCGAAGCACAGCAGGAACAGGTGGCGCAGCCAGCGCGGCTTTACATTGATCGAGTTGATGATCGTCGTGGCGAT
CATCGGGATATTGGCGGCGATCGCGATTCCGGCGTATCAGGACTACACGATTCGGTCACGCGTGACCGAAGGCCTGTCGC
TGGCTGCGCAGGCCAAGGCGCTGGTGGTTGAGAATGCTGCCAACGCCCAGAGTAGCCTGTCAGTCGGTTCTGCATCGTTG
TCGGCCAGCAGGAACGTCAGTGCGCTGAATATTGATTCGTCCACGGGCGAAATCACGGTGGTCTATGCATCTGCTGTTAC
GGGAGCGGGGGCGAATACGTTGGTATTGACGCCGTACTCCGGAAGCTCGTCATCACTTGCCAATTTGCAGGCGGGTTCAG
CGCCGCCGGCAATGGTGCTGTGGGTGTGTGCGGCCGCCGGCAAGTCGATGCCGGTCAGCACCGTCAGCCAGAAAACGACG
GCTACGCTGCCACCGAAGTACGCGCCCGCAGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

56.25

100

0.572
  pilA2 Legionella pneumophila str. Paris

47.273

95.376

0.451
  pilA2 Legionella pneumophila strain ERS1305867

46.667

95.376

0.445
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.436

100

0.422
  comP Acinetobacter baylyi ADP1

40.244

94.798

0.382

Multiple sequence alignment