Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5677_RS13070 Genome accession   NZ_CP039251
Coordinates   3149799..3151505 (+) Length   568 a.a.
NCBI ID   WP_062843519.1    Uniprot ID   -
Organism   Psychrobacter sp. PAMC27889     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3144799..3156505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5677_RS13040 (E5677_13040) nusA 3144982..3146466 (-) 1485 WP_062843522.1 transcription termination factor NusA -
  E5677_RS13045 (E5677_13045) rimP 3146654..3147154 (-) 501 WP_062843521.1 ribosome maturation factor RimP -
  E5677_RS13060 (E5677_13060) secG 3148011..3148319 (-) 309 WP_025652589.1 preprotein translocase subunit SecG -
  E5677_RS13065 (E5677_13065) tpiA 3148535..3149341 (-) 807 WP_062843520.1 triose-phosphate isomerase -
  E5677_RS13070 (E5677_13070) pilB 3149799..3151505 (+) 1707 WP_062843519.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E5677_RS13075 (E5677_13075) pilC 3151724..3152947 (+) 1224 WP_062843518.1 type II secretion system F family protein Machinery gene
  E5677_RS13080 (E5677_13080) pilD 3152955..3153854 (+) 900 WP_062843517.1 prepilin peptidase Machinery gene
  E5677_RS13085 (E5677_13085) coaE 3153985..3154668 (+) 684 WP_062843516.1 dephospho-CoA kinase -
  E5677_RS13090 (E5677_13090) rlmB 3154631..3155401 (-) 771 WP_062843515.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  E5677_RS13095 (E5677_13095) - 3155614..3155949 (-) 336 WP_062843514.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62943.47 Da        Isoelectric Point: 5.3464

>NTDB_id=357622 E5677_RS13070 WP_062843519.1 3149799..3151505(+) (pilB) [Psychrobacter sp. PAMC27889]
MSVTTSKYGGLAQQLISEGVVSEANMKTAQTESQQQQIGLVPYLVDNKLADASHLAQMLSQAFGDPLFDLDALNLDVIPR
DLVDEKIVRKFNALPLFKRGQRLFIALSDPTRVDAIDAIAFNSRLSIETIVVEEDKLKRHIEKIYADTMQSFDSFSDSDL
NVGFEENEEEGEVKLTDSVDEAPVVKFVNKMLVDAIRMGASDLHFEPYEKTYRVRFRVDGVMQKMSNPPVQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKSKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIEALGYEPDQKEMFLEALHKPQG
MLLITGPTGSGKTVSLYTGINILNTGATNISTAEDPVEINLEGINQVNVNPKVGLTFANALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPINIPRQSLLELGFKD
VDLDNPDNIIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVTSIQ
EMMRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=357622 E5677_RS13070 WP_062843519.1 3149799..3151505(+) (pilB) [Psychrobacter sp. PAMC27889]
ATGTCTGTCACAACGAGTAAATATGGCGGGTTGGCGCAGCAGCTAATCAGTGAAGGTGTCGTTAGCGAAGCGAATATGAA
AACGGCGCAAACCGAATCTCAGCAGCAGCAAATAGGATTAGTGCCGTATCTGGTAGACAACAAACTGGCCGACGCGTCCC
ACCTTGCTCAGATGCTGTCACAGGCCTTTGGCGATCCTTTATTCGATTTAGATGCGCTCAATCTTGATGTCATTCCAAGA
GATTTGGTGGACGAAAAAATCGTCCGTAAATTTAATGCCTTGCCACTCTTTAAGCGAGGACAGCGTTTATTTATTGCGTT
AAGTGACCCAACCCGTGTCGATGCGATTGATGCCATCGCCTTTAACTCTCGACTTTCTATTGAAACCATTGTCGTCGAAG
AAGACAAGCTTAAAAGGCATATCGAAAAAATTTATGCTGATACTATGCAAAGTTTCGATAGCTTCTCTGACAGTGATCTA
AACGTTGGTTTTGAAGAAAACGAAGAAGAAGGTGAAGTCAAACTTACAGACAGCGTGGATGAGGCCCCTGTGGTAAAGTT
TGTCAATAAAATGCTGGTGGATGCCATTCGTATGGGCGCCTCTGATTTGCATTTTGAGCCCTATGAAAAAACGTATCGTG
TTCGCTTTCGTGTCGATGGCGTGATGCAGAAAATGTCTAATCCGCCCGTACAGCTGGCAGGAAAAATCGCAGCGCGCTTA
AAGGTCATGTCACAGATGGACATCTCAGAGCGCCGTGTGCCACAAGATGGACGTATTAAACTTAAAATATCTAAAAGTAA
AGCCATTGATTTTCGTGTGAACTCTTTACCAACACTGTTCGGTGAAAAAATCGTTCTGCGTATTTTGGATCCTTCTTCAG
CCATGCTAGGTATTGAAGCCTTGGGTTATGAGCCAGATCAAAAAGAGATGTTCTTAGAAGCGCTGCATAAGCCGCAAGGC
ATGCTATTGATTACTGGTCCTACGGGTTCCGGTAAAACTGTTTCATTATATACAGGTATCAATATATTAAATACAGGCGC
GACCAATATCTCAACTGCTGAAGACCCTGTCGAGATCAATTTAGAGGGTATCAATCAGGTTAACGTCAATCCCAAAGTGG
GGTTGACCTTTGCTAACGCCCTTAAGTCCTTTTTGCGCCAAGATCCTGATATTGTGATGGTTGGGGAGATTCGTGACCTT
GAAACGGCAGAGATTGCCATTAAAGCCGCTCAGACGGGTCACATGGTACTCTCAACCTTGCACACCAATTCCGCTCCTGA
AACATTGACCCGTCTGCGTAACATGGGTGTGGCTTCTTTCAATATCGCTACTTCCGTCAACTTGGTTATCGCTCAGCGCT
TGGCACGACGTCTATGCAAAAACTGTAAAAAGCCTATCAATATCCCTCGCCAAAGCCTACTTGAGCTTGGATTTAAGGAT
GTGGATTTAGACAATCCAGATAATATCATTTATGAACCAGTAGGTTGTAATGAGTGCCGTGAAGGCTATAAAGGACGTGT
CGGTATTTATGAAGTCATGAAAGTCACACCTGATATTTCACGCATTATTATGGAAGATGGAAATGCGATAGATATCAAAG
ATGCGGCACTCAAAAATGGTTTCCGAGATCTGCGCCGCTCAGGTGTTTTAAAGGTTTTACAAGGGGTCACCAGTATCCAA
GAAATGATGCGAGTAACCTCGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.007

100

0.685
  pilB Acinetobacter baylyi ADP1

65.548

99.648

0.653
  pilB Legionella pneumophila strain ERS1305867

51.916

100

0.525
  pilF Neisseria gonorrhoeae MS11

48.587

99.648

0.484
  pilB Vibrio cholerae strain A1552

50.654

94.19

0.477
  pilB Vibrio campbellii strain DS40M4

47.764

98.415

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

49.353

95.246

0.47

Multiple sequence alignment