Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5093_RS01590 Genome accession   NZ_CP039031
Coordinates   339060..340766 (-) Length   568 a.a.
NCBI ID   WP_016659655.1    Uniprot ID   A0A6C0YJ55
Organism   Acinetobacter indicus strain yy_1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334060..345766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5093_RS01560 (E5093_01560) - 334208..334531 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  E5093_RS01565 (E5093_01565) rlmB 334637..335389 (+) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  E5093_RS01570 (E5093_01570) - 335407..336327 (+) 921 WP_168396667.1 DMT family transporter -
  E5093_RS01575 (E5093_01575) coaE 336324..336932 (-) 609 WP_177155274.1 dephospho-CoA kinase -
  E5093_RS01580 (E5093_01580) pilD 336943..337803 (-) 861 WP_177155275.1 prepilin peptidase Machinery gene
  E5093_RS01585 (E5093_01585) pilC 337803..339029 (-) 1227 WP_177155276.1 type II secretion system F family protein Machinery gene
  E5093_RS01590 (E5093_01590) pilB 339060..340766 (-) 1707 WP_016659655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E5093_RS01595 (E5093_01595) tpiA 341056..341847 (+) 792 WP_005181780.1 triose-phosphate isomerase -
  E5093_RS01600 (E5093_01600) secG 341861..342190 (+) 330 WP_005181783.1 preprotein translocase subunit SecG -
  E5093_RS01625 (E5093_01625) rimP 343059..343583 (+) 525 WP_005181784.1 ribosome maturation factor RimP -
  E5093_RS01630 (E5093_01630) nusA 343616..345100 (+) 1485 WP_005181786.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63758.17 Da        Isoelectric Point: 4.8722

>NTDB_id=356825 E5093_RS01590 WP_016659655.1 339060..340766(-) (pilB) [Acinetobacter indicus strain yy_1]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDNDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=356825 E5093_RS01590 WP_016659655.1 339060..340766(-) (pilB) [Acinetobacter indicus strain yy_1]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGATAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTAGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATAACGATGAGCCCCAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGGTCGATGCAATCCGTATGGGCGCCTCGGATTTACACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTACTGCGCCAGATTGCCAATCCTCCGTTACAGCTGGCCACACGGCTCGCCTCACGTTTA
AAAGTTATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCACTGCCCACCCTGTTCGGGGAAAAAATTGTACTGCGTATTCTCGATCCATCTAGTG
CCATGCTGGGGATTGATGCACTCGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTGCTGATTACCGGTCCGACCGGTTCTGGTAAAACCGTGTCTTTATATACCGGCTTAAATATCCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTAGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCCAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGTCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGACCTG
GAAACCGCAGAAATTGCCATTAAAGCCGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCTGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCTTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTGTGTCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAGCTCGGCTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTGATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGCAATGCCTTGCAGATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.526

100

0.808
  pilB Acinetobacter baylyi ADP1

78.07

100

0.783
  pilF Neisseria gonorrhoeae MS11

51.964

98.592

0.512
  pilB Legionella pneumophila strain ERS1305867

50.53

99.648

0.504
  pilB Vibrio cholerae strain A1552

49.912

100

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.553

98.415

0.488
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.258

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366

Multiple sequence alignment