Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GPY16_RS14605 Genome accession   NZ_CP046832
Coordinates   3131706..3133205 (-) Length   499 a.a.
NCBI ID   WP_158105538.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 06-2410     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3126706..3138205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY16_RS14575 gspK 3127303..3128325 (-) 1023 WP_026130546.1 type II secretion system minor pseudopilin GspK -
  GPY16_RS14580 gspJ 3128318..3129019 (-) 702 WP_017789551.1 type II secretion system minor pseudopilin GspJ -
  GPY16_RS14585 gspI 3129006..3129368 (-) 363 WP_038941656.1 type II secretion system minor pseudopilin GspI -
  GPY16_RS14590 gspH 3129361..3130065 (-) 705 WP_045595057.1 type II secretion system minor pseudopilin GspH -
  GPY16_RS14595 gspG 3129992..3130435 (-) 444 WP_017420501.1 type II secretion system major pseudopilin GspG -
  GPY16_RS14600 gspF 3130489..3131706 (-) 1218 WP_158105537.1 type II secretion system inner membrane protein GspF -
  GPY16_RS14605 pilB/pilB1 3131706..3133205 (-) 1500 WP_158105538.1 type II secretion system ATPase GspE Machinery gene
  GPY16_RS14610 gspD 3133205..3135226 (-) 2022 WP_017420503.1 type II secretion system secretin GspD -
  GPY16_RS14615 gspC 3135263..3136138 (-) 876 WP_157401904.1 type II secretion system protein GspC -
  GPY16_RS14620 hslR 3136523..3136909 (+) 387 WP_011078946.1 ribosome-associated heat shock protein Hsp15 -
  GPY16_RS14625 hslO 3136941..3137816 (+) 876 WP_017420504.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55904.18 Da        Isoelectric Point: 5.9486

>NTDB_id=356199 GPY16_RS14605 WP_158105538.1 3131706..3133205(-) (pilB/pilB1) [Vibrio vulnificus strain 06-2410]
MVDILDTAPSYRRLPFSFANRFKMVLEIEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQDIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=356199 GPY16_RS14605 WP_158105538.1 3131706..3133205(-) (pilB/pilB1) [Vibrio vulnificus strain 06-2410]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AATCGAGCACCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTGCGTCGTG
TGCTCAAACAGACCTTTGTCCCTCAAGACATTGCCGCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGTCAGTTGATGGAGGACATTGGCTCGGATGATGACTTCTTCTCTTTAGCCGAAGAGCTGCCGCAAGA
TGAAGATTTGCTCGAAACGGAAGACGATGCACCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GTGCGTCGGATATTCACATCGAAACGTTTGAGAAAATTTTGTCGATTCGTTTCCGTGTCGATGGCGTTTTACGCGATGTG
TTGTCTCCGAGCCGCAAACTGGCGCCGCTGTTGGTCTCTCGCGTTAAAGTCATGGCCAAACTCGACATCGCGGAAAAACG
AGTGCCACAAGATGGCCGTATTTCACTGCGTATCGGTGGTCGCGCGGTCGATGTGCGTGTATCGACCATGCCTTCATCAC
ACGGAGAACGTGTGGTGATGCGTTTGCTCGACAAAAACGCCACTCGTCTTGACCTGCATAGCTTGGGCATGACGCCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGTCCTCACGGCATTATTTTGGTCACAGGTCCAACGGGTTCGGGTAAATC
CACCACCTTGTACGCTGGCTTGCAAGAGCTGAACAGCAACGAGCGCAACATCTTAACGGTGGAAGACCCGATCGAATTTG
ATATTGATGGCATCGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCACGCGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAAATTCGTGACTTGGAAACCGCGCAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAACACCGCCGTAGGTGCGATTACCCGTCTGCGAGACATGGGCATTGAGCCTT
TTTTGATCTCTTCTTCACTGCTGGGCGTGCTGGCTCAGCGCTTGGTGCGGACCTTATGCCAAGATTGTAAAGAGCCGTAT
GAGGCCGACAAAGAGCAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACTTTATATCGTGCCAAGGGGTGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACGGGTATCCATGAGCTGTTGATGGTGGACGATAAAGTCCAAGAGCTGA
TCCATGCAGAGGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCGAGCATTCGCAGCGATGGTTTAGAC
AAAGTGTTGCAAGGCGTGACCTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

43.346

100

0.457
  pilF Thermus thermophilus HB27

44.989

93.988

0.423
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.718

78.156

0.381
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363