Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E0W60_RS25335 Genome accession   NZ_CP038635
Coordinates   3125801..3127570 (+) Length   589 a.a.
NCBI ID   WP_133098009.1    Uniprot ID   A0A4P7LDZ4
Organism   Cupriavidus oxalaticus strain X32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3120801..3132570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0W60_RS25300 (E0W60_25300) proB 3120984..3122102 (-) 1119 WP_135705968.1 glutamate 5-kinase -
  E0W60_RS25305 (E0W60_25305) obgE 3122150..3123247 (-) 1098 WP_133098446.1 GTPase ObgE -
  E0W60_RS25310 (E0W60_25310) rpmA 3123360..3123620 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  E0W60_RS25315 (E0W60_25315) rplU 3123660..3123971 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  E0W60_RS25320 (E0W60_25320) - 3124167..3124460 (-) 294 WP_135705969.1 hypothetical protein -
  E0W60_RS25325 (E0W60_25325) ispB 3124459..3125388 (+) 930 WP_133098448.1 octaprenyl diphosphate synthase -
  E0W60_RS25335 (E0W60_25335) pilF 3125801..3127570 (+) 1770 WP_133098009.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  E0W60_RS25340 (E0W60_25340) - 3127640..3128911 (+) 1272 WP_133098008.1 type II secretion system F family protein -
  E0W60_RS25345 (E0W60_25345) pilD 3128915..3129817 (+) 903 WP_135705970.1 A24 family peptidase Machinery gene
  E0W60_RS25350 (E0W60_25350) coaE 3129869..3130507 (+) 639 WP_133098006.1 dephospho-CoA kinase -
  E0W60_RS25355 (E0W60_25355) zapD 3130745..3131503 (+) 759 WP_133098005.1 cell division protein ZapD -
  E0W60_RS25360 (E0W60_25360) - 3131521..3131712 (+) 192 WP_135705971.1 DNA gyrase inhibitor YacG -
  E0W60_RS25365 (E0W60_25365) - 3131887..3132324 (-) 438 WP_133098004.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 589 a.a.        Molecular weight: 64827.58 Da        Isoelectric Point: 7.7992

>NTDB_id=355242 E0W60_RS25335 WP_133098009.1 3125801..3127570(+) (pilF) [Cupriavidus oxalaticus strain X32]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLAVFAADKYQLPLLDLNQYNLTKVPPGLAGNREF
HAHRLLPLGRRENRLVLGLSDPSNQAGLDAIKEKYKVPVEAVVVEHDKLMKHVRSAGEALGTLKNISPVQAERKMIEYDP
VAAAGAQRNRTAADAIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDPKDNKDSKDAKDPKDKDARETVEKAVDFRVSTLPTLFGEKIVMRILESSTDKLDI
DQLGYEAQQKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFA
AALRSFLRQDPDVIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRL
CTCKRAGELPRETLLDAGFREHDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARKD
GVLSLREAGLLKVRAGVTSLEEVLATTNT

Nucleotide


Download         Length: 1770 bp        

>NTDB_id=355242 E0W60_RS25335 WP_133098009.1 3125801..3127570(+) (pilF) [Cupriavidus oxalaticus strain X32]
ATGACACTCGGACTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCCCGTGA
AAAGCAGTCGCAGCTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCGGTGTTTGCCGCGGACA
AGTACCAGCTGCCGCTGCTCGACCTGAACCAGTACAACCTGACCAAGGTGCCGCCGGGGCTGGCGGGCAACCGCGAATTC
CATGCGCACCGCCTGCTGCCGCTGGGCCGCCGCGAAAACCGGCTGGTGCTCGGGCTGTCCGATCCGTCCAACCAGGCCGG
CCTGGACGCGATCAAGGAAAAGTACAAGGTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCACGTGC
GCTCCGCCGGCGAGGCGCTGGGCACGCTGAAGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAGTACGACCCG
GTGGCGGCCGCCGGCGCGCAGCGCAACCGCACTGCCGCCGATGCCATCGACGACGCGCCCGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAAGCCTTCCACCGCGGCGCGTCCGACCTGCATTTCGAGCCGTTCGAGACCTTCTACCGGATCCGCTTCC
GCGTGGACGGCGTGCTGCAGGAGGTGGCAAGGCCACCGCTGGATATCCGCGACAAGATTGCCACCCGCATCAAGGTGCTG
TCCCGGCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGATCCGAA
GGACAACAAAGACAGCAAGGACGCGAAGGATCCCAAGGACAAGGACGCCAGGGAAACGGTCGAGAAAGCCGTCGATTTCC
GCGTCTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGCGGATCCTGGAATCGTCGACCGACAAGCTCGACATC
GACCAGCTCGGCTACGAGGCGCAACAGAAGGCGCTGCTGCTGGACGTGATCAAGCGTCCGTACGGCATGGTGCTGGTGAC
CGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCTGAACCTGCTGAACCAGGGCGACATCAATATCTCGA
CCGCAGAGGACCCGGCCGAAATCCAGCTGCCCGGCATCAACCAGGTCAACGTCAACGACAAGGCCGGCCTGACCTTTGCC
GCGGCGCTGCGTTCGTTCCTGCGGCAGGATCCGGACGTCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGATAT
CTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACGCTGCACACCAATGACGCCCCCACCACGCTGACGCGGC
TGATGAACATGGGCGTGGCGCCGTTCAACATCGCTTCCAGCGTGCTGATGATCACGGCGCAGCGGCTGGCGCGCCGGCTG
TGCACATGCAAGCGCGCCGGCGAGCTGCCGCGCGAGACGCTGCTGGATGCCGGCTTTCGCGAACACGACCTGGACGGCAG
CTGGCAGCCCTACCATCCGGTCGGGTGCGAGCGCTGCAACGGCAGCGGCTACAAGGGCCGCTGCGGTATCTACCAGGTCA
TGCCGATCACCGAGGCCATGCAGCAGATCATCCTGTCGCACGGCACCGCCCTGCAGATCGCCGAGCAGGCGCGCAAGGAT
GGCGTGCTATCGTTGCGCGAAGCCGGGCTGCTGAAGGTCAGGGCGGGCGTCACGTCACTCGAAGAGGTGCTGGCGACCAC
GAATACGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(284-479)

Type II/IV secretion system protein (T2SSE)

(187-259)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7LDZ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

49.663

100

0.501
  pilB Acinetobacter baumannii D1279779

50

99.151

0.496
  pilB Acinetobacter baylyi ADP1

49.735

95.925

0.477
  pilB Legionella pneumophila strain ERS1305867

48.587

96.095

0.467
  pilB Vibrio cholerae strain A1552

43.559

100

0.436
  pilB Vibrio campbellii strain DS40M4

44.324

94.228

0.418
  pilB Vibrio parahaemolyticus RIMD 2210633

44.404

94.058

0.418
  pilF Thermus thermophilus HB27

37.669

100

0.379

Multiple sequence alignment