Detailed information NTG0355243

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E0W60_RS25345
• Genome accession: NZ_CP038635
• Coordinates: 3128915..3129817 (+)
• Length: 300 a.a.
• NCBI ID: WP_135705970.1
• Uniprot ID: A0A4P7LFG6
• Organism: Cupriavidus oxalaticus strain X32
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3123915..3134817

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0W60_RS25320 (E0W60_25320)	-	3124167..3124460 (-)	294	WP_135705969.1	hypothetical protein	-
E0W60_RS25325 (E0W60_25325)	ispB	3124459..3125388 (+)	930	WP_133098448.1	octaprenyl diphosphate synthase	-
E0W60_RS25335 (E0W60_25335)	pilF	3125801..3127570 (+)	1770	WP_133098009.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
E0W60_RS25340 (E0W60_25340)	-	3127640..3128911 (+)	1272	WP_133098008.1	type II secretion system F family protein	-
E0W60_RS25345 (E0W60_25345)	pilD	3128915..3129817 (+)	903	WP_135705970.1	A24 family peptidase	Machinery gene
E0W60_RS25350 (E0W60_25350)	coaE	3129869..3130507 (+)	639	WP_133098006.1	dephospho-CoA kinase	-
E0W60_RS25355 (E0W60_25355)	zapD	3130745..3131503 (+)	759	WP_133098005.1	cell division protein ZapD	-
E0W60_RS25360 (E0W60_25360)	-	3131521..3131712 (+)	192	WP_135705971.1	DNA gyrase inhibitor YacG	-
E0W60_RS25365 (E0W60_25365)	-	3131887..3132324 (-)	438	WP_133098004.1	NUDIX domain-containing protein	-
E0W60_RS25370 (E0W60_25370)	-	3132321..3133226 (-)	906	WP_133098003.1	ATP-binding protein	-
E0W60_RS25375 (E0W60_25375)	argJ	3133287..3134525 (-)	1239	WP_135705972.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 32089.33 Da
• Isoelectric Point: 8.9049

>NTDB_id=355243 E0W60_RS25345 WP_135705970.1 3128915..3129817(+) (pilD) [Cupriavidus oxalaticus strain X32]
MALAYSVSPFPAGSGQLLQALAALPPAFMVAVTAVLGLVVGSFLNVVIHRLPRMMEREEANYIAELRGDPLPYPARYNLM
VPRSACPHCGHAIAPWENVPVLSWLALRGRCSACKTRISARYPLVELACGVLSGLVAWRFGPGVQTLAALVLVWALLALT
LIDADTQLLPDQITLPLLWLGLVLNLGGLFVPLADAVIGAAAGYLVLWAAYWLFRLVRGKEGMGFGDFKLMAALGAWFGW
QALPALVLLSSVAGVVFGLASIALRRQDRDTPFPFGPFIALAGLVVLVFGSRVLPLTVLP

Nucleotide

• Length: 903 bp

>NTDB_id=355243 E0W60_RS25345 WP_135705970.1 3128915..3129817(+) (pilD) [Cupriavidus oxalaticus strain X32]
ATGGCGCTGGCGTATTCCGTTTCGCCGTTTCCCGCCGGCAGCGGGCAGTTGCTGCAGGCACTGGCGGCGCTGCCGCCTGC
CTTCATGGTGGCGGTGACGGCCGTGCTGGGTCTGGTGGTGGGCAGTTTCCTCAACGTGGTGATCCACCGGCTGCCGCGCA
TGATGGAGCGCGAGGAAGCCAACTATATCGCCGAGCTGCGCGGCGATCCGCTGCCCTACCCCGCCCGCTACAACCTGATG
GTGCCGCGCTCGGCCTGTCCGCACTGCGGCCACGCGATCGCGCCCTGGGAAAACGTGCCGGTGCTGAGTTGGCTGGCGCT
GCGCGGGCGCTGCTCGGCCTGCAAGACCCGGATCAGCGCGCGCTATCCGCTGGTGGAGCTGGCCTGCGGCGTGCTGAGCG
GGCTGGTGGCGTGGCGCTTCGGCCCTGGCGTGCAGACATTGGCGGCGCTGGTGCTGGTCTGGGCGCTGCTGGCGCTGACC
CTGATCGATGCCGACACCCAGCTGCTGCCGGACCAGATCACGCTGCCGCTGCTGTGGCTGGGACTGGTGCTGAACCTGGG
CGGGTTGTTCGTGCCGCTGGCCGATGCCGTGATCGGTGCCGCCGCCGGCTACCTGGTGCTGTGGGCGGCGTACTGGCTGT
TCCGGCTGGTGCGTGGCAAGGAAGGCATGGGTTTCGGCGACTTCAAGCTGATGGCGGCACTCGGCGCGTGGTTTGGCTGG
CAGGCGCTGCCGGCGCTGGTGCTGCTGTCATCGGTGGCCGGCGTGGTGTTCGGGCTGGCCAGCATCGCGCTGCGCCGGCA
GGATCGCGACACGCCCTTCCCCTTCGGCCCCTTTATCGCGCTGGCGGGCCTGGTGGTGCTGGTGTTCGGCTCCCGTGTGC
TGCCGCTGACCGTACTGCCGTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (150-259)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (35-140)

3D structure

• AlphaFold DB: A0A4P7LFG6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.044	90.667	0.49
pilD	Vibrio campbellii strain DS40M4	52.19	91.333	0.477
pilD	Neisseria gonorrhoeae MS11	50.36	92.667	0.467
pilD	Acinetobacter baumannii D1279779	46.691	90.667	0.423
pilD	Acinetobacter nosocomialis M2	46.324	90.667	0.42