Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY00_RS08705 Genome accession   NZ_CP046747
Coordinates   1854126..1854536 (-) Length   136 a.a.
NCBI ID   WP_019829614.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3541-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1849126..1859536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY00_RS08685 coaE 1849614..1850222 (-) 609 WP_000011550.1 dephospho-CoA kinase -
  GPY00_RS08690 pilD 1850219..1851094 (-) 876 WP_019829617.1 A24 family peptidase Machinery gene
  GPY00_RS08695 pilC 1851152..1852378 (-) 1227 WP_019829616.1 type II secretion system F family protein Machinery gene
  GPY00_RS08700 pilB 1852431..1854119 (-) 1689 WP_019829615.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY00_RS08705 pilA 1854126..1854536 (-) 411 WP_019829614.1 pilin Machinery gene
  GPY00_RS08710 nadC 1854782..1855672 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY00_RS08715 ampD 1855970..1856515 (+) 546 WP_019829613.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY00_RS08720 - 1856585..1857965 (-) 1381 Protein_1614 IS4 family transposase -
  GPY00_RS08725 fldB 1858104..1858622 (-) 519 WP_019828645.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14290.50 Da        Isoelectric Point: 7.7804

>NTDB_id=354790 GPY00_RS08705 WP_019829614.1 1854126..1854536(-) (pilA) [Vibrio cholerae strain 3541-04]
MKAYKNKQQKGFTLIELMIVVAIIGVLAAIAVPAYKDYVKKSQASSALATLKSLVTPAELLIQSDGTISGGLSVLGISAG
SNTLGTLSVGSDNTSLVFEFKSGDMAKENLTMSREDTGWVCKRSDDTKIPGIDGCQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=354790 GPY00_RS08705 WP_019829614.1 1854126..1854536(-) (pilA) [Vibrio cholerae strain 3541-04]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGTGTGTT
AGCAGCGATTGCTGTGCCTGCTTACAAAGATTACGTCAAAAAAAGCCAAGCTTCATCAGCGTTAGCCACCTTGAAAAGCC
TCGTAACACCGGCAGAACTACTCATTCAATCGGATGGCACTATTAGTGGTGGTCTGAGCGTTTTAGGTATATCCGCAGGT
TCAAATACTTTAGGAACGCTTTCTGTTGGTAGCGACAACACATCTTTAGTATTCGAATTCAAATCTGGTGATATGGCAAA
AGAGAATCTCACGATGAGTCGCGAAGATACAGGGTGGGTTTGTAAGAGAAGTGATGATACAAAAATTCCAGGTATTGATG
GTTGCCAATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(41-124)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.471
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.559

100

0.426
  pilA Vibrio cholerae strain A1552

40.559

100

0.426
  pilA Vibrio cholerae C6706

40.559

100

0.426
  pilA Pseudomonas aeruginosa PAK

38.255

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.762

100

0.397