Detailed information
Bioinformatically predicted
Overview
| Name | pilB | Type | Machinery gene |
| Locus tag | E4186_RS02280 | Genome accession | NZ_CP038444 |
| Coordinates | 468219..469925 (-) | Length | 568 a.a. |
| NCBI ID | WP_042649025.1 | Uniprot ID | - |
| Organism | Aeromonas media strain T5-8 | ||
| Function | power the assembly of type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| IScluster/Tn | 470563..472185 | 468219..469925 | flank | 638 |
Gene organization within MGE regions
Location: 468219..472185
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E4186_RS02280 (E4186_02305) | pilB | 468219..469925 (-) | 1707 | WP_042649025.1 | PilB family type IVa pilus assembly ATPase TapB | Machinery gene |
| E4186_RS22440 | - | 470223..470390 (-) | 168 | Protein_435 | prepilin-type N-terminal cleavage/methylation domain-containing protein | - |
| E4186_RS02290 (E4186_02320) | tnpA | 470563..470988 (-) | 426 | WP_041205574.1 | IS200/IS605-like element ISAeme8 family transposase | - |
| E4186_RS02295 (E4186_02325) | - | 471046..472185 (+) | 1140 | WP_041206992.1 | RNA-guided endonuclease TnpB family protein | - |
Sequence
Protein
Download Length: 568 a.a. Molecular weight: 62696.11 Da Isoelectric Point: 5.8224
>NTDB_id=354378 E4186_RS02280 WP_042649025.1 468219..469925(-) (pilB) [Aeromonas media strain T5-8]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDDNVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDDNVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN
Nucleotide
Download Length: 1707 bp
>NTDB_id=354378 E4186_RS02280 WP_042649025.1 468219..469925(-) (pilB) [Aeromonas media strain T5-8]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCCTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAATGACATCCTCGACAGCAAGGCCCTAG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTTATCGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCTCTGGAGGACTTTGGCTTCAGCTTCGGTCTGCATACCGAAGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAAATC
TCGGAACTCGAGGTCTCTGATGAGAGTTCGCGGCTCGACGACAACGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTAGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACAGTATCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCAGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCACGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTTGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCCTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAATGACATCCTCGACAGCAAGGCCCTAG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTTATCGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCTCTGGAGGACTTTGGCTTCAGCTTCGGTCTGCATACCGAAGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAAATC
TCGGAACTCGAGGTCTCTGATGAGAGTTCGCGGCTCGACGACAACGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTAGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACAGTATCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCAGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCACGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTTGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilB | Acinetobacter baumannii D1279779 |
54.85 |
99.824 |
0.548 |
| pilB | Acinetobacter baylyi ADP1 |
54.529 |
99.12 |
0.54 |
| pilB | Legionella pneumophila strain ERS1305867 |
53.901 |
99.296 |
0.535 |
| pilB | Vibrio cholerae strain A1552 |
52.473 |
99.648 |
0.523 |
| pilB | Vibrio parahaemolyticus RIMD 2210633 |
49.378 |
99.12 |
0.489 |
| pilB | Vibrio campbellii strain DS40M4 |
49.378 |
99.12 |
0.489 |
| pilF | Neisseria gonorrhoeae MS11 |
46.032 |
99.824 |
0.46 |
| pilF | Thermus thermophilus HB27 |
39.736 |
93.486 |
0.371 |
| pilB/pilB1 | Synechocystis sp. PCC 6803 |
38.043 |
97.183 |
0.37 |