Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GO602_RS19435 Genome accession   NZ_CP046602
Coordinates   4202905..4204374 (+) Length   489 a.a.
NCBI ID   WP_024915396.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CMC-115     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4197905..4209374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO602_RS19415 (GO602_19395) gbpA 4198402..4199904 (+) 1503 WP_121366718.1 N-acetylglucosamine-binding protein GbpA -
  GO602_RS19420 (GO602_19400) - 4199971..4200486 (+) 516 WP_121304959.1 cytochrome b -
  GO602_RS19425 (GO602_19405) - 4200492..4201004 (+) 513 WP_121274609.1 type II secretion system protein N -
  GO602_RS19430 (GO602_19410) gspD 4201001..4202908 (+) 1908 WP_121131035.1 type II secretion system secretin GspD -
  GO602_RS19435 (GO602_19415) pilB 4202905..4204374 (+) 1470 WP_024915396.1 type II secretion system ATPase GspE Machinery gene
  GO602_RS19440 (GO602_19420) gspF 4204381..4205598 (+) 1218 WP_024915397.1 type II secretion system inner membrane protein GspF -
  GO602_RS19445 (GO602_19425) gspG 4205614..4206048 (+) 435 WP_024915398.1 type II secretion system major pseudopilin GspG -
  GO602_RS19450 (GO602_19430) gspH 4206050..4206556 (+) 507 WP_058140079.1 type II secretion system minor pseudopilin GspH -
  GO602_RS19455 (GO602_19435) gspI 4206543..4206926 (+) 384 WP_024915400.1 type II secretion system minor pseudopilin GspI -
  GO602_RS19460 (GO602_19440) gspJ 4206923..4207522 (+) 600 WP_058140081.1 type II secretion system minor pseudopilin GspJ -
  GO602_RS19465 (GO602_19445) gspK 4207515..4208387 (+) 873 WP_024915402.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 53466.29 Da        Isoelectric Point: 6.6042

>NTDB_id=353879 GO602_RS19435 WP_024915396.1 4202905..4204374(+) (pilB) [Pseudomonas aeruginosa strain CMC-115]
MSAGARLPFSYARRHGVLLDDDQLYCRADSALAILAEVRRHAGAARCRVLGAEDFAQRLAAHYRDGRDDAMQMAEGLGEQ
IAEQDDLASLAEQLPQTTDLLDQEDDAPIIRLINAILGEAVKAKASDIHLETFEQHLSVRLRVDGLLREILRPRRELAGL
LVSRIKVMAKLDIAEKRIPQDGRIALRIAGHEVDVRVSTLPSAHGERVVMRLLDKQAGRLDLGRLGMAESTRERLEGALR
RPHGILLVTGPTGSGKTTTLYAGLASLNSLARNILTIEDPVEYHLPGIGQTQVNAKVDMTFARGLRAILRQDPDVVMVGE
IRDRETAEIAVQASLTGHLVLSTLHTNSAVGAVTRLVDMGVEPFLLCTSLLGVLAQRLVRVLCPQCKRPAPADGGACERL
DLDPLAAPTLFHPQGCPACQHSGYRGRQGIYELVLFDEGLRQRVHEGAGEAELARYARQHGHSLFADGREKVLLGVTSLE
ELLRVTQED

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=353879 GO602_RS19435 WP_024915396.1 4202905..4204374(+) (pilB) [Pseudomonas aeruginosa strain CMC-115]
GTGAGCGCTGGCGCCCGACTGCCGTTCAGCTATGCGCGCCGCCATGGCGTGCTGCTGGACGACGACCAGTTGTATTGCCG
CGCCGACAGTGCGCTGGCGATCCTCGCCGAAGTGCGCCGCCACGCCGGCGCGGCGCGCTGCCGGGTGCTCGGCGCGGAGG
ATTTCGCCCAGCGCCTGGCCGCGCACTACCGCGACGGACGCGACGACGCCATGCAGATGGCCGAGGGCCTCGGCGAGCAG
ATCGCCGAGCAGGACGACCTGGCGAGCCTCGCCGAGCAGCTACCGCAGACCACCGACCTGCTGGACCAGGAAGACGACGC
GCCGATCATCCGCCTGATCAACGCGATCCTCGGCGAAGCGGTAAAGGCCAAGGCCTCGGACATCCACCTGGAGACCTTCG
AGCAGCACCTTTCGGTGCGGCTGCGGGTCGACGGGCTGCTCCGCGAGATTCTCCGCCCGCGCCGCGAGCTGGCCGGCCTG
CTGGTTTCGCGGATCAAGGTGATGGCCAAGCTGGATATTGCCGAGAAGCGCATCCCCCAGGACGGACGCATCGCCCTGCG
CATCGCCGGCCACGAAGTCGACGTGCGCGTCTCGACCCTGCCCTCGGCCCATGGCGAGCGCGTGGTGATGCGCCTGCTCG
ACAAGCAGGCCGGGCGCCTCGACCTGGGGCGCCTGGGCATGGCCGAGAGCACCCGCGAACGCCTGGAAGGCGCCCTGCGC
CGGCCCCACGGGATTCTCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCACCACGCTCTACGCCGGGCTGGCCAGCCT
CAACAGCCTGGCCCGCAACATCCTCACCATCGAGGACCCGGTGGAATACCACCTCCCCGGGATCGGCCAGACCCAGGTCA
ACGCCAAGGTCGACATGACCTTCGCCCGCGGTCTGCGCGCCATTCTCCGGCAGGATCCGGACGTGGTGATGGTCGGCGAG
ATCCGTGACCGCGAGACCGCCGAGATCGCCGTACAGGCGTCGCTGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAA
CAGCGCAGTAGGCGCGGTCACCCGCCTGGTGGACATGGGCGTAGAGCCGTTCCTGCTCTGTACCTCGCTGCTCGGCGTGC
TGGCACAGCGCCTGGTACGGGTGCTCTGCCCACAGTGCAAGCGGCCGGCGCCGGCCGATGGCGGCGCCTGCGAGCGACTC
GACCTCGACCCGCTGGCGGCGCCGACGTTGTTCCACCCGCAGGGCTGCCCGGCCTGCCAGCACAGCGGCTACCGCGGCCG
TCAGGGCATCTATGAACTGGTGCTGTTCGACGAAGGCCTGCGCCAGCGGGTCCACGAAGGCGCTGGCGAGGCGGAGCTAG
CCCGCTACGCCCGCCAGCACGGCCACAGCCTGTTCGCCGACGGCCGCGAAAAAGTGCTGCTGGGCGTCACCAGCCTCGAG
GAACTGCTGCGCGTGACCCAGGAGGATTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(115-381)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

48.63

89.571

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

50.122

84.049

0.421
  pilB Legionella pneumophila strain ERS1305867

42.38

97.955

0.415
  pilB Vibrio cholerae strain A1552

47.867

86.299

0.413
  pilB Acinetobacter baumannii D1279779

44.966

91.411

0.411
  pilB/pilB1 Synechocystis sp. PCC 6803

41.86

96.728

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.875

85.072

0.399
  pilB Acinetobacter baylyi ADP1

48.454

79.346

0.384
  pilF Thermus thermophilus HB27

44.417

84.254

0.374
  pilF Neisseria gonorrhoeae MS11

45.178

80.573

0.364