Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E3983_RS08260 Genome accession   NZ_CP038254
Coordinates   1850320..1852041 (+) Length   573 a.a.
NCBI ID   WP_058502405.1    Uniprot ID   A0AAX1EHT1
Organism   Legionella israelensis strain HL-0427-4011     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1845320..1857041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3983_RS08240 (E3983_08240) - 1847064..1848200 (+) 1137 WP_135060606.1 HlyD family type I secretion periplasmic adaptor subunit -
  E3983_RS08245 (E3983_08245) - 1848222..1848797 (-) 576 WP_058502290.1 hypoxanthine-guanine phosphoribosyltransferase -
  E3983_RS08250 (E3983_08250) - 1848920..1849246 (-) 327 WP_058502289.1 DUF1820 family protein -
  E3983_RS08255 (E3983_08255) - 1849372..1850151 (-) 780 WP_167554556.1 methyltransferase domain-containing protein -
  E3983_RS08260 (E3983_08260) pilB 1850320..1852041 (+) 1722 WP_058502405.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E3983_RS08265 (E3983_08265) pilC 1852047..1853267 (+) 1221 WP_135060608.1 type II secretion system F family protein Machinery gene
  E3983_RS08270 (E3983_08270) - 1853271..1854131 (+) 861 WP_058502286.1 A24 family peptidase -
  E3983_RS08275 (E3983_08275) - 1854357..1854947 (-) 591 WP_058502285.1 hypothetical protein -
  E3983_RS08280 (E3983_08280) - 1854959..1855366 (-) 408 WP_131780737.1 hypothetical protein -
  E3983_RS08285 (E3983_08285) - 1855731..1856546 (+) 816 WP_058502283.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63199.58 Da        Isoelectric Point: 6.0483

>NTDB_id=352779 E3983_RS08260 WP_058502405.1 1850320..1852041(+) (pilB) [Legionella israelensis strain HL-0427-4011]
MATLAEEQRLQGIGQLLVLEQLLEKDKTLKYQQLASADNVTLLQYLVNNSILSAVNIASAIARNFGVPLLDLDCIEFDSI
PSTLVSEKLIRRHHMIPLFHRGNHLYLATDDPSQQASLKEIQFHTGLHAHPVVVETDKLAQLIEHLLREKESQGLHDYVD
ESVDLDGLEISSEEEEHDIESAASASEDAPVVKFVNKILLDAIKNSVSDIHFEPYEQTYRIRYRQDGVLTEVASPPVSLS
GRITARIKVMSNLDISERRVPQDGRFKMKLSKSRAIDFRVSTCPTVGGEKVVMRILDPGSIKLGIEALGFSDIQKTHFLK
SIERPQGMILVTGPTGSGKTVTLYTAMNILNTKERNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLVNMGVPSFNIASSVSLIIAQRLARRLCEQCKTKRDDITEKG
MIELGFSEEEAKKPIFKAVGCNQCNGGYRGRVGLFEVMPMSKAIGRLIMSGGNSLDILKQGQDEGMLTIFQSGLKKILEG
VTTIEEVNRVTVD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=352779 E3983_RS08260 WP_058502405.1 1850320..1852041(+) (pilB) [Legionella israelensis strain HL-0427-4011]
ATGGCAACACTCGCAGAAGAACAACGATTGCAAGGCATAGGCCAATTATTGGTTCTTGAGCAACTCCTGGAAAAAGACAA
GACTCTGAAATATCAGCAATTAGCCAGTGCCGATAATGTAACTTTACTCCAATATCTGGTCAATAACAGTATACTCAGTG
CCGTTAATATTGCTTCCGCGATTGCCAGAAATTTTGGTGTACCTTTGTTGGATTTGGACTGTATCGAGTTTGATTCTATA
CCCTCCACACTCGTCAGTGAAAAACTTATCCGGCGCCATCATATGATTCCACTTTTTCACCGCGGTAATCATTTATACCT
TGCAACAGATGATCCCAGTCAACAGGCATCTCTGAAAGAAATACAGTTTCACACCGGTCTGCATGCTCATCCTGTTGTCG
TTGAAACAGACAAGTTAGCACAACTTATTGAGCATTTATTGCGGGAAAAAGAAAGCCAGGGTCTGCATGATTATGTCGAT
GAGTCCGTTGACCTCGATGGCCTTGAAATCAGTTCGGAAGAAGAAGAGCATGATATTGAATCAGCTGCCTCTGCTTCAGA
AGATGCCCCTGTAGTCAAATTTGTAAATAAAATTCTGCTTGATGCCATTAAAAATTCAGTATCGGATATTCATTTTGAAC
CTTATGAACAAACATATAGAATCCGATATCGCCAAGATGGTGTTCTAACTGAAGTCGCATCACCTCCTGTCAGTCTTTCC
GGACGGATCACTGCAAGAATCAAGGTTATGTCAAATCTGGATATTTCTGAAAGACGTGTGCCTCAGGATGGACGCTTTAA
AATGAAATTATCAAAATCGAGAGCTATAGATTTCAGGGTAAGTACCTGCCCCACCGTGGGAGGTGAAAAGGTGGTTATGC
GTATCCTTGATCCTGGAAGTATAAAATTAGGAATCGAAGCTTTGGGATTTAGCGATATTCAAAAAACTCATTTTTTAAAA
TCCATCGAACGCCCGCAAGGTATGATATTGGTAACAGGGCCTACAGGAAGCGGTAAAACCGTAACTTTATATACAGCCAT
GAATATTCTCAATACCAAAGAGAGGAACATCTCTACCGCTGAAGATCCCGTTGAAATTAAAGTCCCCGGTATCAATCAAG
TCAATATTAACCCTAAAGCGGGACTCACTTTTTCTGGTGCACTTCGTTCATTTTTACGGCAAGATCCTGACATCATCATG
GTTGGAGAAATTAGGGATCTTGAAACAGCGGAAATTGCAGTAAAGGCAGCTCAAACAGGCCATCTTGTTTTATCCACTCT
TCACACAAACAGTGCCGCAGAAACATTGACTCGCCTGGTTAACATGGGTGTGCCTTCTTTCAATATTGCAAGCTCGGTAA
GCTTAATCATCGCTCAGCGTCTGGCAAGACGCCTCTGTGAACAATGTAAAACAAAAAGAGACGATATCACTGAGAAAGGA
ATGATTGAATTAGGATTTAGCGAAGAAGAAGCTAAAAAGCCCATATTCAAAGCTGTGGGTTGTAATCAATGCAATGGTGG
CTATCGGGGCAGAGTAGGATTATTTGAAGTCATGCCAATGTCAAAGGCAATAGGAAGACTCATTATGTCAGGAGGGAATT
CCTTGGATATACTCAAACAAGGTCAAGATGAGGGCATGTTAACTATTTTTCAATCCGGATTGAAAAAAATATTGGAAGGG
GTAACGACGATTGAGGAGGTCAACCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

75.436

100

0.756
  pilB Acinetobacter baylyi ADP1

53.68

97.208

0.522
  pilB Acinetobacter baumannii D1279779

55.556

92.67

0.515
  pilF Neisseria gonorrhoeae MS11

47.452

99.302

0.471
  pilB Vibrio cholerae strain A1552

48.429

94.415

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

47.08

95.637

0.45
  pilB Vibrio campbellii strain DS40M4

47.681

94.066

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.927

96.161

0.384
  pilF Thermus thermophilus HB27

38.692

93.368

0.361

Multiple sequence alignment