Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   ES300_RS05785 Genome accession   NZ_CP038251
Coordinates   1166830..1167678 (-) Length   282 a.a.
NCBI ID   WP_000705324.1    Uniprot ID   A0A4K9QSJ2
Organism   Streptococcus pneumoniae strain TVO_1901944     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 1161830..1172678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES300_RS05770 (ES300_06060) - 1162184..1162543 (-) 360 WP_001237219.1 YbaN family protein -
  ES300_RS05775 (ES300_06065) topA 1162649..1164736 (-) 2088 WP_000179650.1 type I DNA topoisomerase -
  ES300_RS05780 (ES300_06070) - 1164903..1165946 (-) 1044 WP_001812957.1 TIGR00341 family protein -
  ES300_RS10680 - 1166524..1166646 (+) 123 WP_001814242.1 hypothetical protein -
  ES300_RS05785 (ES300_06075) dprA/cilB/dalA 1166830..1167678 (-) 849 WP_000705324.1 DNA-processing protein DprA Machinery gene
  ES300_RS05790 (ES300_06080) - 1167774..1168463 (-) 690 WP_000643967.1 sugar phosphate nucleotidyltransferase -
  ES300_RS05795 (ES300_06085) - 1168475..1169353 (-) 879 WP_000837609.1 DMT family transporter -
  ES300_RS05800 (ES300_06090) licA 1169343..1170212 (-) 870 WP_000411201.1 choline kinase LicA -
  ES300_RS05805 (ES300_06095) - 1170229..1171251 (-) 1023 WP_000609915.1 ribitol-5-phosphate dehydrogenase -
  ES300_RS05810 (ES300_06100) - 1171256..1171963 (-) 708 WP_000638497.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31150.79 Da        Isoelectric Point: 5.2061

>NTDB_id=352544 ES300_RS05785 WP_000705324.1 1166830..1167678(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901944]
MKITNYEIYKLKKSSLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPDSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=352544 ES300_RS05785 WP_000705324.1 1166830..1167678(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901944]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAAGTTTGACCAATCAACAGATTTTGAAAGTTCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGATAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4K9QSJ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

99.291

100

0.993
  dprA/cilB/dalA Streptococcus pneumoniae D39

99.291

100

0.993
  dprA/cilB/dalA Streptococcus pneumoniae R6

99.291

100

0.993
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

99.291

100

0.993
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

97.872

100

0.979
  dprA/cilB/dalA Streptococcus mitis SK321

97.163

100

0.972
  dprA Streptococcus mutans UA159

62.5

99.291

0.621
  dprA Lactococcus lactis subsp. cremoris KW2

55

99.291

0.546
  dprA Staphylococcus aureus N315

37.456

100

0.376

Multiple sequence alignment