Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E3Z29_RS27035 Genome accession   NZ_CP038207
Coordinates   5828325..5830025 (+) Length   566 a.a.
NCBI ID   WP_098965662.1    Uniprot ID   -
Organism   Pseudomonas sp. S150     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5823325..5835025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3Z29_RS27015 (E3Z29_27015) - 5823490..5826438 (+) 2949 WP_134787353.1 DUF748 domain-containing protein -
  E3Z29_RS27020 (E3Z29_27020) - 5826438..5826734 (+) 297 WP_134787354.1 DUF2845 domain-containing protein -
  E3Z29_RS27025 (E3Z29_27025) - 5827000..5827356 (-) 357 WP_025108814.1 BON domain-containing protein -
  E3Z29_RS27030 (E3Z29_27030) - 5827656..5828096 (-) 441 WP_098965660.1 pilin -
  E3Z29_RS27035 (E3Z29_27035) pilB 5828325..5830025 (+) 1701 WP_098965662.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E3Z29_RS27040 (E3Z29_27040) pilC 5830028..5831245 (+) 1218 WP_098965663.1 type II secretion system F family protein Machinery gene
  E3Z29_RS27045 (E3Z29_27045) pilD 5831247..5832119 (+) 873 WP_134787355.1 A24 family peptidase Machinery gene
  E3Z29_RS27050 (E3Z29_27050) coaE 5832116..5832739 (+) 624 WP_134787356.1 dephospho-CoA kinase -
  E3Z29_RS27055 (E3Z29_27055) yacG 5832736..5832933 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -
  E3Z29_RS27060 (E3Z29_27060) - 5832940..5833155 (-) 216 WP_003228298.1 hypothetical protein -
  E3Z29_RS27065 (E3Z29_27065) - 5833221..5833910 (-) 690 WP_115988876.1 energy-coupling factor ABC transporter permease -
  E3Z29_RS27070 (E3Z29_27070) - 5834028..5834654 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62479.49 Da        Isoelectric Point: 5.8273

>NTDB_id=352308 E3Z29_RS27035 WP_098965662.1 5828325..5830025(+) (pilB) [Pseudomonas sp. S150]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTEAIEKFFDTHASGLEEMADVDLDG
VDVESVDDSRQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPP
EQIGSFTIYEPVGCDLCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDSQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=352308 E3Z29_RS27035 WP_098965662.1 5828325..5830025(+) (pilB) [Pseudomonas sp. S150]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTTACGGAAAAAAGCGCCCAGCAGGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAAGAGCTCACAGGTGG
CCGAGATCGCTTCCGAGCATTTCGGTATGGCCTTCATGGACCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCTTGTGGCGCCGTGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGTACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCACCGAGGCCATCGAGAAATTCTTCGACACCCACGCCAGCGGCCTCGAAGAAATGGCCGACGTCGATCTCGACGGC
GTGGATGTCGAGTCCGTCGACGACAGCCGCCAGGACGCCATCGGCGGACTCGACGCCGATGACGCGCCGGTCGTGCGTTT
CGTGCACAAGATGCTGCTCGACGCGATCAAGAGTGGCTCCTCTGACCTGCACTTCGAACCTTACGAAAAGACCTTCCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTGGCCAAACCGCCGATCCAGTTGGCCGGGCGAATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATCTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGACCAA
GTCCATCGACTTCCGGGTCAACACTTTACCGACCCTATGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGCATCGATGCATTGGGTTACGAACCGGCGCAGAAAGACCTGTACATGGCCGCGCTCAAGCAGCCCCAAGGG
ATGATTCTGGTAACCGGACCTACCGGGTCGGGGAAAACCGTATCGCTGTACACCGGCCTCAATATTCTCAACACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GGCTGGATTTTGCTCAGGCCCTGCGTTCGTTTCTGCGGCAGGATCCGGACGTGATCATGGTCGGGGAAATCCGCGACCTG
GAAACCGCCGAAATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCCACGCTGCACACCAACAGCGCTGCCGA
AACCCTGACCCGTCTGCACAACATGGGCATTCCCGGGTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCACAGCGCC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAACCGATCGAGATCCCCCGCGAAACACTGATCAAGGAAGGTTTCCCGCCG
GAACAGATCGGCAGTTTCACGATCTATGAGCCGGTCGGTTGCGACCTCTGCAACGGCGGCTACAAGGGACGCGTGGGGAT
TTACGAAGTGGTGAAAAACACCCCTGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACAGCCAGA
TGCGTCGCGACGGCTTCGACGACCTGCGCACCTCGGGCCTGCACAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

55.477

100

0.555
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio campbellii strain DS40M4

49.385

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.117

0.495
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilF Thermus thermophilus HB27

36.738

98.587

0.362

Multiple sequence alignment