Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GNT62_RS21820 Genome accession   NZ_CP046406
Coordinates   4605298..4606806 (-) Length   502 a.a.
NCBI ID   WP_003091377.1    Uniprot ID   A0A0H2ZC25
Organism   Pseudomonas aeruginosa strain SE5458     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4600298..4611806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNT62_RS21790 (GNT62_21725) xcpX 4601020..4602021 (-) 1002 WP_003091371.1 GspK family T2SS minor pseudopilin variant XcpX -
  GNT62_RS21795 (GNT62_21730) xcpW 4602018..4602722 (-) 705 WP_003091372.1 GspJ family T2SS minor pseudopilin variant XcpW -
  GNT62_RS21800 (GNT62_21735) xcpV 4602719..4603108 (-) 390 WP_003091373.1 GspI family T2SS minor pseudopilin variant XcpV -
  GNT62_RS21805 (GNT62_21740) xcpU 4603105..4603623 (-) 519 WP_003091374.1 GspH family T2SS minor pseudopilin variant XcpU -
  GNT62_RS21810 (GNT62_21745) xcpT 4603630..4604076 (-) 447 WP_003130409.1 GspG family T2SS major pseudopilin variant XcpT -
  GNT62_RS21815 (GNT62_21750) xcpS 4604081..4605298 (-) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  GNT62_RS21820 (GNT62_21755) pilB 4605298..4606806 (-) 1509 WP_003091377.1 GspE family T2SS ATPase variant XcpR Machinery gene
  GNT62_RS21825 (GNT62_21760) xcpP 4607026..4607733 (+) 708 WP_003119750.1 type II secretion system protein N -
  GNT62_RS21830 (GNT62_21765) xcpQ 4607738..4609714 (+) 1977 WP_003091380.1 GspD family T2SS secretin variant XcpQ -
  GNT62_RS21835 (GNT62_21770) - 4609801..4610568 (-) 768 WP_003091381.1 SDR family oxidoreductase -
  GNT62_RS21840 (GNT62_21775) - 4610565..4611776 (-) 1212 WP_003091382.1 O-succinylhomoserine sulfhydrylase -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55524.73 Da        Isoelectric Point: 6.2074

>NTDB_id=352081 GNT62_RS21820 WP_003091377.1 4605298..4606806(-) (pilB) [Pseudomonas aeruginosa strain SE5458]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=352081 GNT62_RS21820 WP_003091377.1 4605298..4606806(-) (pilB) [Pseudomonas aeruginosa strain SE5458]
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGTTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTGGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGCGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAACACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGCGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGTGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAGGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZC25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367