Detailed information NTG0351615

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: E3U39_RS17095
• Genome accession: NZ_CP038028
• Coordinates: 3392251..3392865 (+)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain FS1092
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3387251..3397865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3U39_RS17060 (E3U39_17060)	aroE	3387938..3388780 (+)	843	WP_039254404.1	shikimate dehydrogenase	-
E3U39_RS17065 (E3U39_17065)	yhbY	3388774..3389064 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
E3U39_RS17070 (E3U39_17070)	-	3389075..3389644 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
E3U39_RS17075 (E3U39_17075)	yqeK	3389634..3390194 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
E3U39_RS17080 (E3U39_17080)	rsfS	3390212..3390568 (+)	357	WP_007408260.1	ribosome silencing factor	-
E3U39_RS17085 (E3U39_17085)	-	3390565..3391302 (+)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
E3U39_RS17090 (E3U39_17090)	comER	3391371..3392192 (-)	822	WP_015417855.1	late competence protein ComER	-
E3U39_RS17095 (E3U39_17095)	comEA	3392251..3392865 (+)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
E3U39_RS17100 (E3U39_17100)	-	3392932..3393501 (+)	570	WP_003152868.1	ComE operon protein 2	-
E3U39_RS17105 (E3U39_17105)	comEC	3393502..3395853 (+)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
E3U39_RS17110 (E3U39_17110)	-	3395872..3396006 (-)	135	WP_003152870.1	YqzM family protein	-
E3U39_RS17115 (E3U39_17115)	-	3396047..3396201 (+)	155	Protein_3275	hypothetical protein	-
E3U39_RS17120 (E3U39_17120)	holA	3396241..3397282 (+)	1042	Protein_3276	DNA polymerase III subunit delta	-
E3U39_RS17125 (E3U39_17125)	rpsT	3397299..3397565 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=351615 E3U39_RS17095 WP_053573180.1 3392251..3392865(+) (comEA) [Bacillus amyloliquefaciens strain FS1092]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=351615 E3U39_RS17095 WP_053573180.1 3392251..3392865(+) (comEA) [Bacillus amyloliquefaciens strain FS1092]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363