Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DAIF1_RS02730 Genome accession   NZ_CP037883
Coordinates   598477..599604 (-) Length   375 a.a.
NCBI ID   WP_025878450.1    Uniprot ID   A0A1W1H1T2
Organism   Stenotrophomonas indicatrix strain DAIF1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 593477..604604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAIF1_RS02710 (DAIF1_05400) - 593877..594866 (+) 990 WP_080150104.1 fumarylacetoacetate hydrolase family protein -
  DAIF1_RS02715 (DAIF1_05410) maiA 594881..595549 (+) 669 WP_134831962.1 maleylacetoacetate isomerase -
  DAIF1_RS02720 (DAIF1_05420) - 595583..597082 (-) 1500 WP_134831963.1 UdgX family uracil-DNA binding protein -
  DAIF1_RS02725 (DAIF1_05430) - 597063..598346 (-) 1284 WP_059065909.1 putative DNA modification/repair radical SAM protein -
  DAIF1_RS02730 (DAIF1_05440) pilU 598477..599604 (-) 1128 WP_025878450.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DAIF1_RS02735 (DAIF1_05450) - 599726..600091 (+) 366 WP_025878451.1 DUF4398 domain-containing protein -
  DAIF1_RS02740 (DAIF1_05460) - 600088..600972 (+) 885 WP_134831964.1 OmpA family protein -
  DAIF1_RS02745 (DAIF1_05480) - 601296..601532 (+) 237 WP_025878453.1 YdcH family protein -
  DAIF1_RS02750 (DAIF1_05490) - 601884..603254 (+) 1371 WP_134831965.1 pyridoxal-phosphate dependent enzyme -
  DAIF1_RS02755 (DAIF1_05500) - 603378..604562 (+) 1185 WP_025878455.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41371.63 Da        Isoelectric Point: 6.3738

>NTDB_id=350424 DAIF1_RS02730 WP_025878450.1 598477..599604(-) (pilU) [Stenotrophomonas indicatrix strain DAIF1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKNGTIDQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=350424 DAIF1_RS02730 WP_025878450.1 598477..599604(-) (pilU) [Stenotrophomonas indicatrix strain DAIF1]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTGTATCCGCTCGGCAATACCGGCCTGCCGCCGGGAATGGTGAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGGCCGATGCCGGGCGT
TTCCGCGTCAACGTGTTCAAGCAGCGCGGTGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCATCGCAACAGCACCACGACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGCGAGGTCGGGCTCGATACCCATGCCTTCCACAA
TGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGGCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGGCGCCGCCTGCCGGCCACCGAAGTACTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTACACGAGATCAAGGCGGCGATGGAGGCTTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGTTGGCCAAGAACGGCACCATCGATCAGGAAGAAGCACTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGAGGCAGCGGCGAGCATGATCCGTATGCGGACTTCTCGGCCGGCGCGCCGAGCATTACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W1H1T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Vibrio cholerae strain A1552

49.167

96

0.472
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36

Multiple sequence alignment