Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E0Z06_RS02640 Genome accession   NZ_CP037745
Coordinates   551377..553068 (-) Length   563 a.a.
NCBI ID   WP_132581634.1    Uniprot ID   A0A4P6VQY3
Organism   Rheinheimera sp. D18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 546377..558068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0Z06_RS02610 (E0Z06_02610) mutT 547105..547518 (+) 414 WP_132581622.1 8-oxo-dGTP diphosphatase MutT -
  E0Z06_RS02615 (E0Z06_02615) yacG 547515..547742 (-) 228 WP_132581624.1 DNA gyrase inhibitor YacG -
  E0Z06_RS02620 (E0Z06_02620) zapD 547772..548518 (-) 747 WP_132581626.1 cell division protein ZapD -
  E0Z06_RS02625 (E0Z06_02625) coaE 548564..549172 (-) 609 WP_132581628.1 dephospho-CoA kinase -
  E0Z06_RS02630 (E0Z06_02630) pilD 549189..550067 (-) 879 WP_132581630.1 A24 family peptidase Machinery gene
  E0Z06_RS02635 (E0Z06_02635) pilC 550067..551296 (-) 1230 WP_132581632.1 type II secretion system F family protein Machinery gene
  E0Z06_RS02640 (E0Z06_02640) pilB 551377..553068 (-) 1692 WP_132581634.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E0Z06_RS02645 (E0Z06_02645) nadS 553489..553776 (-) 288 WP_132581636.1 NadS family protein -
  E0Z06_RS02650 (E0Z06_02650) - 553778..554131 (-) 354 WP_243695547.1 type II toxin-antitoxin system RelE/ParE family toxin -
  E0Z06_RS02655 (E0Z06_02655) - 554401..554850 (-) 450 WP_347812433.1 pilin -
  E0Z06_RS02660 (E0Z06_02660) - 554853..555278 (-) 426 WP_279432805.1 pilin -
  E0Z06_RS02665 (E0Z06_02665) - 555660..556328 (-) 669 WP_132581638.1 cold shock and DUF1294 domain-containing protein -
  E0Z06_RS02670 (E0Z06_02670) nadC 556442..557311 (-) 870 WP_132581640.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E0Z06_RS02675 (E0Z06_02675) - 557314..557835 (-) 522 WP_132581642.1 TIGR02281 family clan AA aspartic protease -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61623.02 Da        Isoelectric Point: 5.5935

>NTDB_id=349835 E0Z06_RS02640 WP_132581634.1 551377..553068(-) (pilB) [Rheinheimera sp. D18]
MAVTTTSTLFRRLVQLRIIDGDKATKIVNEKGSQFKNLPELLINEKLISAEELASAASGIGHAISLDLDHFDMAYVPEDL
RNEKLIRKTNMLPLGKRGRSMYLAVVDPTDIQTIEDFEFNTGLNAEVIVVDFKKLQALIDKLFDGNLGGDFTDADWDLGS
LGIEQIEEDDGAQPDKDDQPIVAFINKMLLDAIRKGASDLHFEPYEKTYRIRFRIDGILHEIASPPVLLSTRLSARLKVM
ARLDIAEKRVPQDGRIKLKLSAKKSIDFRVSSLPTLWGEKIVMRILDSDSAMLGIDVLGYEEHQKKLYLDALAQPQGMIL
VTGPTGSGKTVSLYTGLAILNTSETNISTAEDPVEINLAGVNQVQVNPRAGLTFATALKAFLRQDPDVVMVGEIRDLETA
EIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPSYNVASSVSLIIAQRLARRLCSQCKQQEVLPEAELLKQGFTAQQL
PSVKIFKPVGCEYCTNGYKGRVGIYEVMPISPKMADIIMQGGNSLDIAAQAQTDGVNNLRQSGLIKAAAGLTSLAEINRV
TKG

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=349835 E0Z06_RS02640 WP_132581634.1 551377..553068(-) (pilB) [Rheinheimera sp. D18]
ATGGCCGTAACCACTACATCTACGTTATTTAGGCGCTTAGTACAGCTACGAATTATTGATGGCGATAAAGCCACGAAAAT
TGTCAACGAAAAAGGCAGCCAGTTTAAAAATCTGCCTGAATTACTAATTAACGAAAAGCTGATTAGCGCAGAAGAGTTAG
CCAGCGCCGCTAGTGGTATTGGCCACGCCATTAGCCTAGATTTAGACCATTTTGATATGGCCTACGTGCCAGAAGATTTA
CGTAACGAAAAGCTAATTCGCAAAACCAATATGCTGCCATTGGGTAAACGTGGCCGCAGTATGTATTTAGCGGTGGTTGA
CCCAACCGACATTCAAACCATTGAAGATTTTGAATTTAACACCGGTTTAAATGCTGAAGTTATTGTGGTTGATTTTAAAA
AACTGCAAGCGCTAATAGACAAGCTGTTTGACGGCAATTTAGGCGGCGACTTTACCGATGCCGATTGGGATTTAGGCTCG
TTAGGTATTGAGCAAATAGAGGAAGACGACGGCGCGCAGCCAGATAAAGACGACCAGCCGATTGTCGCCTTTATTAATAA
AATGCTGTTAGACGCTATTCGTAAGGGCGCGTCAGATTTACACTTTGAACCCTACGAAAAAACCTACCGTATTCGCTTTC
GTATCGACGGCATTTTGCATGAAATAGCCAGCCCACCGGTATTGTTGTCTACTCGCTTATCGGCGCGGTTAAAAGTAATG
GCGCGCTTAGATATTGCGGAAAAACGGGTACCGCAAGATGGCCGAATTAAGCTAAAACTATCGGCAAAGAAGTCTATCGA
CTTTCGTGTCAGTAGCTTACCCACCTTGTGGGGCGAAAAAATAGTAATGCGTATTCTCGACTCTGACAGCGCCATGCTAG
GCATTGATGTGTTGGGCTATGAAGAACACCAGAAAAAACTGTACCTAGACGCGCTAGCCCAACCGCAAGGTATGATTTTA
GTAACCGGCCCTACCGGTTCAGGTAAAACCGTATCGCTGTATACCGGCTTAGCCATTTTAAATACCAGCGAAACCAATAT
TTCTACTGCGGAAGACCCGGTAGAAATTAACTTAGCCGGCGTTAACCAAGTGCAGGTAAACCCGCGTGCTGGCTTAACCT
TTGCTACGGCCTTAAAAGCGTTTTTGCGCCAAGACCCTGACGTGGTAATGGTGGGTGAAATTCGAGATTTAGAAACCGCC
GAAATAGCAATTAAAGCGGCACAAACCGGCCACTTGGTATTATCAACCTTGCACACTAACTCGGCACCGGAAACCCTAAC
TCGCTTACTTAACATGGGTGTACCCTCGTATAACGTAGCCAGCTCGGTATCGCTTATTATTGCCCAGCGCCTAGCGCGGC
GTTTATGTAGCCAATGCAAACAGCAAGAAGTGCTACCAGAGGCAGAGTTATTAAAACAAGGTTTTACTGCCCAGCAGTTG
CCCAGCGTAAAAATATTTAAGCCGGTAGGCTGCGAATATTGCACCAATGGCTATAAAGGCCGGGTGGGTATATATGAAGT
AATGCCCATTAGCCCGAAAATGGCGGATATTATTATGCAAGGCGGTAACTCGTTAGATATTGCCGCGCAGGCCCAAACCG
ATGGGGTTAATAATTTGCGCCAATCGGGCTTAATTAAAGCCGCGGCTGGCCTAACCAGCTTGGCAGAGATTAATCGCGTA
ACCAAAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-144)

Type II/IV secretion system protein (T2SSE)

(187-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P6VQY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.75

99.467

0.584
  pilB Acinetobacter baumannii D1279779

58.273

98.757

0.575
  pilB Legionella pneumophila strain ERS1305867

54.399

98.934

0.538
  pilB Vibrio cholerae strain A1552

53.654

99.645

0.535
  pilB Vibrio campbellii strain DS40M4

50.788

100

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

53.296

94.316

0.503
  pilF Neisseria gonorrhoeae MS11

49.551

98.934

0.49

Multiple sequence alignment