Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GJV02_RS10375 Genome accession   NZ_CP046060
Coordinates   2196091..2197599 (+) Length   502 a.a.
NCBI ID   WP_078451543.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 1811-18R001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2191091..2202599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJV02_RS10355 - 2191116..2192327 (+) 1212 WP_031692600.1 O-succinylhomoserine sulfhydrylase -
  GJV02_RS10360 - 2192324..2193091 (+) 768 WP_031692601.1 SDR family oxidoreductase -
  GJV02_RS10365 xcpQ 2193186..2195159 (-) 1974 WP_023108529.1 GspD family T2SS secretin variant XcpQ -
  GJV02_RS10370 xcpP 2195164..2195871 (-) 708 WP_042173362.1 type II secretion system protein N -
  GJV02_RS10375 pilB 2196091..2197599 (+) 1509 WP_078451543.1 GspE family T2SS ATPase variant XcpR Machinery gene
  GJV02_RS10380 xcpS 2197599..2198816 (+) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  GJV02_RS10385 xcpT 2198821..2199267 (+) 447 WP_003130409.1 GspG family T2SS major pseudopilin variant XcpT -
  GJV02_RS10390 xcpU 2199274..2199792 (+) 519 WP_003110066.1 GspH family T2SS minor pseudopilin variant XcpU -
  GJV02_RS10395 xcpV 2199789..2200178 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  GJV02_RS10400 xcpW 2200175..2200897 (+) 723 WP_031692603.1 GspJ family T2SS minor pseudopilin variant XcpW -
  GJV02_RS10405 xcpX 2200894..2201895 (+) 1002 WP_023436422.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55538.76 Da        Isoelectric Point: 6.2078

>NTDB_id=349771 GJV02_RS10375 WP_078451543.1 2196091..2197599(+) (pilB) [Pseudomonas aeruginosa strain 1811-18R001]
MMTAPLPEIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=349771 GJV02_RS10375 WP_078451543.1 2196091..2197599(+) (pilB) [Pseudomonas aeruginosa strain 1811-18R001]
ATGATGACAGCCCCGTTACCCGAAATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGTTTCGCCAAGCGCCA
GGGTCTGCTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGATGCCTTCAACCAGGCCCTGGCGGCGTCCTAC
CAGCACGATTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCCCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTACTG
CGCGAGGTGCTCGAACCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAACGCATTCCCCAGGATGGACGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGCGAGCGCGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAGCACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACCGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGGCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAAGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGCGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGCGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGTGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367