Detailed information NTG0349643

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EYS44_RS12505
• Genome accession: NZ_CP037417
• Coordinates: 2587592..2588206 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LB002
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2582592..2593206

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EYS44_RS12475 (EYS44_12470)	rpsT	2582892..2583158 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
EYS44_RS12480 (EYS44_12475)	holA	2583175..2584216 (-)	1042	Protein_2401	DNA polymerase III subunit delta	-
EYS44_RS12485 (EYS44_12480)	-	2584256..2584410 (-)	155	Protein_2402	hypothetical protein	-
EYS44_RS12490 (EYS44_12485)	-	2584451..2584585 (+)	135	WP_003152870.1	YqzM family protein	-
EYS44_RS12495 (EYS44_12490)	comEC	2584604..2586955 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EYS44_RS12500 (EYS44_12495)	-	2586956..2587525 (-)	570	WP_003152868.1	ComE operon protein 2	-
EYS44_RS12505 (EYS44_12500)	comEA	2587592..2588206 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
EYS44_RS12510 (EYS44_12505)	comER	2588265..2589086 (+)	822	WP_014305449.1	late competence protein ComER	-
EYS44_RS12515 (EYS44_12510)	-	2589155..2589892 (-)	738	WP_024085626.1	class I SAM-dependent methyltransferase	-
EYS44_RS12520 (EYS44_12515)	rsfS	2589889..2590245 (-)	357	WP_003152864.1	ribosome silencing factor	-
EYS44_RS12525 (EYS44_12520)	yqeK	2590263..2590823 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EYS44_RS12530 (EYS44_12525)	-	2590813..2591382 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
EYS44_RS12535 (EYS44_12530)	yhbY	2591393..2591683 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
EYS44_RS12540 (EYS44_12535)	aroE	2591677..2592519 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=349643 EYS44_RS12505 WP_041482377.1 2587592..2588206(-) (comEA) [Bacillus velezensis strain LB002]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=349643 EYS44_RS12505 WP_041482377.1 2587592..2588206(-) (comEA) [Bacillus velezensis strain LB002]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363