Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   EZY40_RS00595 Genome accession   NZ_CP036530
Coordinates   96694..97728 (+) Length   344 a.a.
NCBI ID   WP_226316548.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain STAB09023     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 91694..102728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZY40_RS00585 (EZY40_00590) - 95362..95727 (+) 366 WP_002986560.1 DUF1033 family protein -
  EZY40_RS00590 (EZY40_00595) comYA 95820..96758 (+) 939 WP_020904831.1 competence type IV pilus ATPase ComGA Machinery gene
  EZY40_RS00595 (EZY40_00600) comYB 96694..97728 (+) 1035 WP_226316548.1 competence type IV pilus assembly protein ComGB Machinery gene
  EZY40_RS00600 (EZY40_00605) comYC 97730..98056 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  EZY40_RS00605 (EZY40_00610) comGD 98031..98459 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EZY40_RS00610 (EZY40_00615) comGE 98416..98700 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  EZY40_RS00615 (EZY40_00620) comYF 98693..99127 (+) 435 WP_020904833.1 competence type IV pilus minor pilin ComGF Machinery gene
  EZY40_RS00620 (EZY40_00625) comGG 99111..99437 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  EZY40_RS00625 (EZY40_00630) comYH 99535..100488 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  EZY40_RS00630 (EZY40_00635) - 100547..101743 (+) 1197 WP_020904834.1 acetate kinase -
  EZY40_RS00635 (EZY40_00640) - 101930..102238 (+) 309 Protein_89 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39676.15 Da        Isoelectric Point: 9.7085

>NTDB_id=349389 EZY40_RS00595 WP_226316548.1 96694..97728(+) (comYB) [Streptococcus pyogenes strain STAB09023]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFTEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMAILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=349389 EZY40_RS00595 WP_226316548.1 96694..97728(+) (comYB) [Streptococcus pyogenes strain STAB09023]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCACAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTAATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGGCTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.277

98.547

0.535
  comYB Streptococcus mutans UA159

54.277

98.547

0.535
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment