Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   EZY40_RS00615 Genome accession   NZ_CP036530
Coordinates   98693..99127 (+) Length   144 a.a.
NCBI ID   WP_020904833.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain STAB09023     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 93693..104127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZY40_RS00585 (EZY40_00590) - 95362..95727 (+) 366 WP_002986560.1 DUF1033 family protein -
  EZY40_RS00590 (EZY40_00595) comYA 95820..96758 (+) 939 WP_020904831.1 competence type IV pilus ATPase ComGA Machinery gene
  EZY40_RS00595 (EZY40_00600) comYB 96694..97728 (+) 1035 WP_226316548.1 competence type IV pilus assembly protein ComGB Machinery gene
  EZY40_RS00600 (EZY40_00605) comYC 97730..98056 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  EZY40_RS00605 (EZY40_00610) comGD 98031..98459 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EZY40_RS00610 (EZY40_00615) comGE 98416..98700 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  EZY40_RS00615 (EZY40_00620) comYF 98693..99127 (+) 435 WP_020904833.1 competence type IV pilus minor pilin ComGF Machinery gene
  EZY40_RS00620 (EZY40_00625) comGG 99111..99437 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  EZY40_RS00625 (EZY40_00630) comYH 99535..100488 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  EZY40_RS00630 (EZY40_00635) - 100547..101743 (+) 1197 WP_020904834.1 acetate kinase -
  EZY40_RS00635 (EZY40_00640) - 101930..102238 (+) 309 Protein_89 hypothetical protein -
  EZY40_RS00640 (EZY40_00645) proC 102321..103091 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16672.29 Da        Isoelectric Point: 10.2526

>NTDB_id=349391 EZY40_RS00615 WP_020904833.1 98693..99127(+) (comYF) [Streptococcus pyogenes strain STAB09023]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRNHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=349391 EZY40_RS00615 WP_020904833.1 98693..99127(+) (comYF) [Streptococcus pyogenes strain STAB09023]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACTCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAATCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment