Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EYC57_RS05455 Genome accession   NZ_CP036256
Coordinates   1132824..1134557 (+) Length   577 a.a.
NCBI ID   WP_053502736.1    Uniprot ID   -
Organism   Xanthomonas oryzae strain BAI23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1127824..1139557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYC57_RS05435 (EYC57_05435) pilA 1128509..1128922 (+) 414 WP_131074740.1 pilin Machinery gene
  EYC57_RS05440 (EYC57_05440) - 1129142..1130653 (+) 1512 WP_341272984.1 phosphoethanolamine transferase -
  EYC57_RS05445 (EYC57_05445) - 1130724..1131293 (+) 570 WP_242628850.1 hypothetical protein -
  EYC57_RS05450 (EYC57_05450) - 1131263..1132789 (+) 1527 WP_053502734.1 hypothetical protein -
  EYC57_RS05455 (EYC57_05455) pilB 1132824..1134557 (+) 1734 WP_053502736.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EYC57_RS05460 (EYC57_05460) - 1134566..1135087 (-) 522 WP_207306597.1 Fic family protein -
  EYC57_RS05465 (EYC57_05465) pilR 1135084..1136478 (-) 1395 WP_131074742.1 sigma-54 dependent transcriptional regulator Regulator
  EYC57_RS05470 (EYC57_05470) - 1136809..1138422 (-) 1614 WP_053502740.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62900.00 Da        Isoelectric Point: 5.6097

>NTDB_id=347725 EYC57_RS05455 WP_053502736.1 1132824..1134557(+) (pilB) [Xanthomonas oryzae strain BAI23]
MNSVSTTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKKLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=347725 EYC57_RS05455 WP_053502736.1 1132824..1134557(+) (pilB) [Xanthomonas oryzae strain BAI23]
ATGAATTCAGTATCTACAACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACAGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAAAAGAAGCTGG
TGTCGGCATCGCAGCTGGCGGCGGCCAATGCAGTGGAATTCGGCATGCCACTGATGGATGTGTCTGTGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCATGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACTCAGACCCGTGCACTAGATGACATCAAGTTCCATACGAATCTGGTCGTCGAGCCGA
TTCTTGTCGATGAGGACCAAATTCGTCGCACATTGGAGCAGTGGCAGGCAAGCAATGCCTCATTTGGATCATCGCTTGGC
AACGATGATGAGGAAATGGGAGATCTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAAGTGCTGGTGGATGCGATCCGGCGGGGGGCCTCGGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGTTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGATTTCCGTGTCAGTACGTTGCCGACCCTGTTCGGTGAGA
AGGTGGTGCTGCGTATTCTGGACGGTAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAAAAG
CTGTTCCTGGATGCAATTCATAAGCCTTACGGGATGGTGCTGGTCACCGGGCCCACCGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATTCGCCTGCCTG
GCGTCAACCAGGTCCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GATATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCACAGACCATCGCGCGTCTGATGAACATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCAAGTCGACGTTGCCG
GAGCATGCACTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTCGCGAATATGGAGCTTTATGAAGCCGTGGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGTGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCTCAGAAGATCGGCATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGCGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.44

0.556
  pilB Acinetobacter baylyi ADP1

56.338

98.44

0.555
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilB Vibrio cholerae strain A1552

49.74

100

0.497
  pilF Neisseria gonorrhoeae MS11

49.12

98.44

0.484
  pilB Vibrio campbellii strain DS40M4

46.087

99.653

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

46.004

97.574

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

91.508

0.36

Multiple sequence alignment