Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EWH46_RS02620 Genome accession   NZ_CP035708
Coordinates   534016..535809 (-) Length   597 a.a.
NCBI ID   WP_149502534.1    Uniprot ID   A0A5C1PVX8
Organism   Sphaerotilus sulfidivorans strain D-507     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 529016..540809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWH46_RS02590 (EWH46_02590) - 529869..530138 (-) 270 WP_219340951.1 hypothetical protein -
  EWH46_RS02595 (EWH46_02595) - 530223..530990 (-) 768 WP_149502529.1 hypothetical protein -
  EWH46_RS02600 (EWH46_02600) - 531084..531497 (-) 414 WP_149502530.1 type II toxin-antitoxin system HicB family antitoxin -
  EWH46_RS02605 (EWH46_02605) - 531509..531688 (-) 180 WP_149502531.1 type II toxin-antitoxin system HicA family toxin -
  EWH46_RS02610 (EWH46_02610) - 531726..532370 (-) 645 Protein_471 tyrosine-type recombinase/integrase -
  EWH46_RS20660 - 532378..532500 (-) 123 WP_256365868.1 hypothetical protein -
  EWH46_RS02615 (EWH46_02615) pilC 532701..533927 (-) 1227 WP_149502533.1 type II secretion system F family protein Machinery gene
  EWH46_RS02620 (EWH46_02620) pilB 534016..535809 (-) 1794 WP_149502534.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EWH46_RS02625 (EWH46_02625) htpG 536135..538027 (+) 1893 WP_149502535.1 molecular chaperone HtpG -
  EWH46_RS02630 (EWH46_02630) - 538128..538568 (+) 441 WP_149502536.1 MerR family transcriptional regulator -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 65008.55 Da        Isoelectric Point: 5.4844

>NTDB_id=344013 EWH46_RS02620 WP_149502534.1 534016..535809(-) (pilB) [Sphaerotilus sulfidivorans strain D-507]
MRFSPSDPCLPPFATVSVILAVAAEILPDGPASTLSGVARVLVLAGKLPSRIAEDLSRVARDQRRSFVSAVVGANALSAY
DLACSLSQVLGLPLLDLDAIDINRLPQNLVDARTLSQYQVLVLGRRGSRLFIGGADPTDQEAVDRIKFATQLTPEWVVLE
YDKLARIVDAKSTSAGDALDSIVSSGDFDFEVEEQDAGTKADDEVASAEVEDAPVVRFLQKMLVDAINMRASDLHFEPYE
NTYRVRFRIDGELREITQPPIAIKDKLSSRIKVISKLDISEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILD
PSSARLGVDALGYERDEKERLLSAVRRPYGMILVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGVNQVNVN
DKAGMNFAVALKAFLRQDPDVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLLNMGVPAFNIAASVILIT
AQRLARRLCENCKAPADYPREALLRAGFAADDLDGSWRPYRAIGCSACNNGYRGRVGLYQVMPITEAVQRIILAQGNAID
IAEQARRDGVRDLRQAGLLKVRHGVTTLEEVISVTNQ

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=344013 EWH46_RS02620 WP_149502534.1 534016..535809(-) (pilB) [Sphaerotilus sulfidivorans strain D-507]
ATGCGTTTTTCGCCTTCGGACCCTTGTCTACCTCCTTTCGCAACCGTCTCCGTCATCCTTGCCGTGGCAGCAGAAATTCT
TCCTGATGGACCCGCCAGCACCCTGTCTGGCGTGGCACGCGTGCTGGTGCTCGCCGGCAAGCTGCCCTCGCGGATCGCGG
AAGACCTGTCGCGGGTGGCGCGGGACCAGCGGCGCAGCTTCGTGTCGGCGGTGGTCGGCGCCAATGCGCTGAGCGCCTAT
GACCTGGCCTGCTCGCTGTCGCAGGTGCTCGGGCTGCCGCTGCTCGATCTCGACGCGATCGACATCAACCGGCTGCCGCA
GAACCTGGTCGATGCCCGCACGCTGTCGCAATATCAGGTGCTGGTGCTGGGCCGGCGCGGCAGCCGGCTGTTCATCGGCG
GCGCCGACCCGACCGACCAGGAAGCGGTCGACCGGATCAAGTTCGCCACCCAGCTCACGCCCGAGTGGGTGGTGCTCGAG
TACGACAAGCTCGCCCGCATCGTCGACGCCAAGTCGACCTCGGCCGGCGATGCGCTCGACTCGATCGTGTCCTCCGGCGA
CTTCGACTTCGAGGTCGAGGAGCAGGATGCCGGCACCAAGGCCGACGACGAGGTCGCCTCCGCCGAGGTCGAGGACGCGC
CGGTGGTGCGCTTCCTGCAGAAGATGCTGGTCGACGCGATCAACATGCGCGCCTCCGACCTGCACTTCGAACCCTACGAG
AACACCTACCGGGTGCGGTTCCGCATCGACGGCGAGCTGCGCGAGATCACCCAGCCGCCGATCGCGATCAAGGACAAGCT
GTCCTCGCGCATCAAGGTCATCTCCAAGCTCGACATCTCGGAGAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGAAGT
TCGGCAGCAAGGCGATCGACTTCCGGGTCTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCCGGATTCTCGAC
CCGTCCTCGGCGCGGCTGGGCGTGGACGCGCTCGGCTACGAGCGCGACGAGAAGGAGCGGCTGCTGTCGGCGGTGCGCCG
CCCCTACGGCATGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAAACGGTGTCGCTCTACACCTGCCTGAACATTCTCA
ACCAGCCCGGCGTCAACATCTCCACCGTCGAGGATCCGGCGGAAATCAACCTGCCCGGCGTCAACCAGGTCAATGTCAAC
GACAAGGCGGGCATGAACTTCGCCGTCGCGCTGAAGGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAAAT
CCGCGACCTGGAAACCGCCGACATCGCGATCAAGGCGGCGCAGACCGGTCACCTGGTGCTGTCGACGCTGCACACCAACG
ACGCACCGACGACGCTGACCCGGCTGCTGAACATGGGCGTGCCGGCCTTCAACATCGCCGCCAGCGTGATCCTGATCACC
GCGCAGCGCCTGGCGCGGCGGCTGTGCGAGAACTGCAAGGCACCGGCCGACTATCCGCGCGAGGCCCTGCTGCGGGCCGG
CTTCGCCGCCGACGATCTGGACGGCAGCTGGCGGCCCTACCGGGCGATCGGCTGCTCGGCCTGCAACAACGGCTACCGCG
GCCGCGTCGGCCTGTACCAGGTCATGCCGATCACCGAGGCGGTCCAGCGCATCATCCTCGCCCAGGGCAACGCGATCGAC
ATCGCCGAGCAGGCGCGGCGCGACGGCGTGCGCGATCTGCGCCAGGCCGGACTGCTCAAGGTGCGCCACGGCGTGACGAC
ACTGGAAGAAGTGATTTCCGTCACCAACCAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(75-169)

Type II/IV secretion system protein (T2SSE)

(221-487)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C1PVX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.858

94.64

0.491
  pilB Acinetobacter baylyi ADP1

50.788

95.645

0.486
  pilF Neisseria gonorrhoeae MS11

53.51

88.275

0.472
  pilB Legionella pneumophila strain ERS1305867

49.47

94.807

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.359

94.305

0.437
  pilB Vibrio campbellii strain DS40M4

46.004

94.305

0.434
  pilB Vibrio cholerae strain A1552

47.909

88.107

0.422

Multiple sequence alignment