Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ET948_RS06455 Genome accession   NZ_CP035697
Coordinates   1377692..1379380 (-) Length   562 a.a.
NCBI ID   WP_154115268.1    Uniprot ID   -
Organism   Vibrio cincinnatiensis strain 2409-02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1372692..1384380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET948_RS06430 yacG 1373898..1374095 (-) 198 WP_078926027.1 DNA gyrase inhibitor YacG -
  ET948_RS06435 zapD 1374153..1374893 (-) 741 WP_078926028.1 cell division protein ZapD -
  ET948_RS06440 coaE 1374919..1375533 (-) 615 WP_154115266.1 dephospho-CoA kinase -
  ET948_RS06445 - 1375539..1376404 (-) 866 Protein_1192 prepilin peptidase -
  ET948_RS06450 pilC 1376445..1377671 (-) 1227 WP_154115267.1 type II secretion system F family protein Machinery gene
  ET948_RS06455 pilB 1377692..1379380 (-) 1689 WP_154115268.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ET948_RS06460 - 1379383..1379808 (-) 426 WP_154115269.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ET948_RS06465 nadC 1380005..1380895 (-) 891 WP_154115270.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ET948_RS06470 ampD 1381092..1381637 (+) 546 WP_154115271.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ET948_RS06475 fldB 1381644..1382162 (-) 519 WP_154115272.1 flavodoxin FldB -
  ET948_RS06480 xerD 1382306..1383214 (+) 909 WP_154115273.1 site-specific tyrosine recombinase XerD -
  ET948_RS06485 dsbC 1383230..1383973 (+) 744 WP_154115274.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62411.30 Da        Isoelectric Point: 5.1427

>NTDB_id=343877 ET948_RS06455 WP_154115268.1 1377692..1379380(-) (pilB) [Vibrio cincinnatiensis strain 2409-02]
MLTNLIAILRQAQLMSLAQEQAVVDHIQTSGASVPEALLALDIFHHHDLTEHLSQIFGLPETDLTRFDYASLCQQLGLRE
LITQYQALPLQRNGQVLTLAVSDPTHLQIEDEFRFATGLQIELVLADYKALQAAIRRLYGRTINHATAQGKEISQDELAD
LVQISDDEKQSVEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYEQQYRIRLRCDGILVQIQQPASHLGRRLAARIKI
LAKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSSASLDIDKLGYNDTQKQLYLNALKQPQGMI
LMTGPTGSGKTVSLYTGLSILNTEDVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKEAYFPSLTEQQQFALDADQ
TIYQAHSNGCNECTNGYSGRVGIYEVMAFEPQLAEAIMQKASITQIEAIAQTNGMQTLQQSGIEKLKIGITSIQELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=343877 ET948_RS06455 WP_154115268.1 1377692..1379380(-) (pilB) [Vibrio cincinnatiensis strain 2409-02]
ATGCTTACCAACCTTATTGCTATCTTGCGACAAGCTCAACTTATGAGTTTGGCGCAAGAGCAAGCCGTGGTCGACCATAT
CCAAACTTCCGGTGCCAGTGTACCGGAAGCTTTATTGGCTCTGGATATTTTTCACCATCATGATTTAACCGAACACCTAA
GCCAAATATTTGGTCTGCCCGAAACCGATTTAACTCGCTTTGACTACGCTTCATTGTGTCAACAACTGGGTTTGCGAGAG
CTAATAACACAATACCAAGCCTTACCATTACAGCGGAACGGACAGGTACTGACCTTAGCCGTATCCGACCCCACACATCT
ACAAATTGAAGATGAATTTCGCTTTGCCACCGGACTGCAAATTGAACTAGTACTGGCGGACTATAAAGCCTTACAAGCGG
CCATTCGTAGATTGTATGGTCGAACAATCAACCATGCTACGGCGCAAGGTAAAGAGATCAGTCAAGATGAACTGGCCGAT
TTGGTACAGATCTCGGATGATGAAAAGCAATCCGTTGAAGATCTCAGCCAAGATGACTCTCCAGTGAGTCGCTTTATCAA
CCAAATATTATTGGATGCCGTCCGTAAAGGGGCCTCCGACATACACTTTGAGCCCTACGAACAGCAATACCGCATTCGTC
TGCGCTGTGACGGTATTCTGGTGCAGATCCAACAACCCGCAAGCCACTTAGGACGACGGCTCGCTGCACGGATAAAAATT
CTCGCTAAACTGGATATTGCTGAACGCCGGCTCCCACAAGATGGGCGAATAAAACTGCGCTTAAATGCAGATACCGCCAT
CGATATGCGAGTTTCAACCCTTCCTACATTATGGGGAGAGAAAATTGTCCTACGCTTACTCGATAGCAGTAGCGCAAGTT
TAGACATTGATAAGTTAGGTTATAACGATACACAAAAACAGCTTTACCTAAACGCGTTGAAACAGCCGCAAGGGATGATC
CTGATGACGGGGCCAACAGGAAGTGGCAAAACGGTTTCACTTTATACAGGCTTAAGCATTCTTAATACTGAAGATGTTAA
TATCTCTACCGCAGAAGATCCGGTGGAGATCAACCTTCCTGGCATTAATCAAGTCCAAGTTCAACCCAAAATTGGCTTTG
GCTTTGCCCAAGCGCTACGCTCATTTTTGCGTCAAGACCCTGATATTGTCATGGTTGGTGAAATCCGCGATTTAGAAACC
GCAGAAATCGCAGTCAAAGCCGCTCAAACAGGACACTTGGTTCTGTCAACGTTACACACCAACTCAGCCGCAGAAACCGT
CATTCGCTTAGCCAATATGGGCATAGAACCCTTTAACCTCGCCTCTTCACTCAGTTTAATCATTGCTCAGCGCTTAGCCA
GACGGTTATGTCCACACTGCAAAGAAGCCTATTTTCCAAGCTTAACCGAGCAACAACAGTTTGCGTTAGACGCCGATCAA
ACCATTTACCAAGCCCATTCGAATGGCTGTAACGAATGCACCAATGGTTACAGTGGACGAGTCGGTATTTATGAAGTGAT
GGCCTTTGAGCCTCAACTGGCTGAAGCCATTATGCAAAAAGCTTCCATTACTCAAATTGAAGCCATCGCTCAAACCAATG
GCATGCAAACTTTGCAGCAATCTGGCATAGAAAAACTCAAAATCGGCATCACCAGTATTCAAGAATTACAGCGTGTACTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

81.673

100

0.817
  pilB Vibrio campbellii strain DS40M4

72.242

100

0.722
  pilB Vibrio parahaemolyticus RIMD 2210633

70.641

100

0.706
  pilB Legionella pneumophila strain ERS1305867

52.529

91.459

0.48
  pilB Acinetobacter baumannii D1279779

51.257

91.993

0.472
  pilB Acinetobacter baylyi ADP1

51.262

91.637

0.47
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

38.545

97.865

0.377

Multiple sequence alignment