Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ETN89_RS07600 Genome accession   NZ_CP035457
Coordinates   1624333..1626024 (-) Length   563 a.a.
NCBI ID   WP_086367529.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain KC-Na-NB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1619333..1631024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETN89_RS07570 (ETN89_07570) mutT 1619842..1620264 (-) 423 WP_129382700.1 8-oxo-dGTP diphosphatase MutT -
  ETN89_RS07575 (ETN89_07575) yacG 1620352..1620561 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  ETN89_RS07580 (ETN89_07580) zapD 1620570..1621307 (-) 738 WP_005297594.1 cell division protein ZapD -
  ETN89_RS07585 (ETN89_07585) coaE 1621375..1621977 (-) 603 WP_086367532.1 dephospho-CoA kinase -
  ETN89_RS07590 (ETN89_07590) pilD 1621979..1622857 (-) 879 WP_086367531.1 A24 family peptidase Machinery gene
  ETN89_RS07595 (ETN89_07595) pilC 1623040..1624266 (-) 1227 WP_086367530.1 type II secretion system F family protein Machinery gene
  ETN89_RS07600 (ETN89_07600) pilB 1624333..1626024 (-) 1692 WP_086367529.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ETN89_RS07605 (ETN89_07605) - 1626038..1626490 (-) 453 WP_086367528.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ETN89_RS07610 (ETN89_07610) ampD 1626877..1627455 (+) 579 WP_086367527.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ETN89_RS07615 (ETN89_07615) pdhR 1627815..1628588 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62552.12 Da        Isoelectric Point: 5.2594

>NTDB_id=342591 ETN89_RS07600 WP_086367529.1 1624333..1626024(-) (pilB) [Photobacterium damselae subsp. damselae strain KC-Na-NB1]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITIPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDYEACCKAVYQRD
LVLRHRALPLAQNADTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDEDLN
NLVDMGDDELDQTTDLGQDTEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTPNLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDTLGYNQQQKAAYLNALQQPQGM
ILITGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPAG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=342591 ETN89_RS07600 WP_086367529.1 1624333..1626024(-) (pilB) [Photobacterium damselae subsp. damselae strain KC-Na-NB1]
ATGCACACCACTCTCGCCTCGATTCTGCACCAGGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGATTCCCTCCGCACTGATCCATCTGGGTATATTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATATTTGGTCTTACCCTCGTATCAGTCCATGACTACGATTATGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGTCATCGCGCACTACCCCTCGCCCAAAATGCGGACACACTGTTTTTAGGGGTCAGCGATCCATGCAATAT
CGACGGGCAAGATGAGTTCCGTTTTGCCACTGGCCGTAATATTGAACCGCTACTGCTGGAAAATAAGCAGCTCGAATCGG
CTATCCGTCGTATTTATGGTCGCGAGTTGGGCGAAACCACCAATATGGCCAGTCGTGATGTCAGCGATGAGGATCTCAAT
AACTTAGTCGATATGGGGGATGACGAGCTGGATCAAACCACAGATCTAGGCCAAGATACCGAACCTGTTACCCGTTATAT
TAACCAAGTGCTGCTCGATGCAGTACGTAAAAAAGCCTCCGATATTCACTTTGAACCTTATGAGACCAGCTATCGGATCC
GCTTTCGTTGCGATGGGATCTTACACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTTTCTACCCGACTCAAA
GTGATGTCACGCCTAAATATTGCCGAACGACGCCAACCGCAAGATGGACGCATAAAACTCAAGCTCACACCCAATTTGGC
AGTAGATCTGCGGGTTTCTACCCTGCCCACTTTATGGGGTGAAAAAGTGGTGCTGCGTATTTTAGACAGCTCCAGTGCCA
ACTTAGACATTGATACTTTAGGTTACAACCAACAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAACCGCAAGGGATG
ATCTTAATAACAGGGCCGACGGGCAGCGGCAAAACCGTTTCGCTTTATACCGGACTTAACATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCAGAGGATCCAGTAGAGATCAACTTACCCGGCATTAATCAGGTTCACATTAACAATCAAGCAGGAC
TGGGGTTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCCGATGTGGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACTGCCTCTATTGCCGTTAAAGCAGCGCAAACCGGACACTTAGTACTATCGACCTTGCACACCAATTCTGCCGCCGAAAC
CATTACCCGTTTAACCAATATGGGTATTGAAGACTTTAACCTAGCCTCATCACTGACACTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTAGATGAGATAGACGGCTTTATGCAAGACAAACTGCGCATTCCGGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGTGTCGGTATCTATGAAGT
GATGCCATTTAGTCGCCGACTTGCTCAAGCTCTGGTTGATGGCGCTACCACCTTAGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTGACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.812

100

0.618
  pilB Vibrio parahaemolyticus RIMD 2210633

61.634

100

0.616
  pilB Vibrio campbellii strain DS40M4

60.213

100

0.602
  pilB Acinetobacter baylyi ADP1

49.818

97.336

0.485
  pilB Legionella pneumophila strain ERS1305867

50.369

96.27

0.485
  pilB Acinetobacter baumannii D1279779

51.631

92.54

0.478
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.197

92.895

0.364

Multiple sequence alignment