Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   ETT59_RS02650 Genome accession   NZ_CP035440
Coordinates   506308..508206 (+) Length   632 a.a.
NCBI ID   WP_111681221.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm124     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 490991..535890 506308..508206 within 0


Gene organization within MGE regions


Location: 490991..535890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT59_RS02580 (ETT59_02580) - 491574..492056 (-) 483 WP_002988938.1 S-ribosylhomocysteine lyase -
  ETT59_RS02585 (ETT59_02585) - 492130..493461 (-) 1332 WP_136276603.1 GntP family permease -
  ETT59_RS02590 (ETT59_02590) - 493532..494311 (-) 780 WP_023611644.1 3-hydroxybutyrate dehydrogenase -
  ETT59_RS02595 (ETT59_02595) - 494344..495003 (-) 660 WP_002988946.1 3-oxoacid CoA-transferase subunit B -
  ETT59_RS02600 (ETT59_02600) - 495005..495655 (-) 651 WP_002983642.1 CoA transferase subunit A -
  ETT59_RS02605 (ETT59_02605) - 495679..496866 (-) 1188 WP_136300706.1 acetyl-CoA C-acetyltransferase -
  ETT59_RS02610 (ETT59_02610) - 497067..497960 (+) 894 WP_129321336.1 LysR family transcriptional regulator -
  ETT59_RS02615 (ETT59_02615) - 498090..499697 (+) 1608 WP_014635620.1 ribonuclease Y -
  ETT59_RS02620 (ETT59_02620) gmk 499807..500442 (+) 636 WP_002983649.1 guanylate kinase -
  ETT59_RS02625 (ETT59_02625) rpoZ 500458..500775 (+) 318 WP_014635619.1 DNA-directed RNA polymerase subunit omega -
  ETT59_RS02630 (ETT59_02630) - 500840..503224 (+) 2385 WP_136276607.1 primosomal protein N' -
  ETT59_RS02635 (ETT59_02635) fmt 503286..504221 (+) 936 WP_053308253.1 methionyl-tRNA formyltransferase -
  ETT59_RS02640 (ETT59_02640) rsmB 504211..505533 (+) 1323 WP_136306021.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ETT59_RS02645 (ETT59_02645) - 505571..506311 (+) 741 WP_136276609.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ETT59_RS02650 (ETT59_02650) pknB 506308..508206 (+) 1899 WP_111681221.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ETT59_RS02655 (ETT59_02655) liaF 508329..509021 (+) 693 WP_011054833.1 cell wall-active antibiotics response protein LiaF -
  ETT59_RS02660 (ETT59_02660) - 509018..510022 (+) 1005 WP_002991890.1 sensor histidine kinase -
  ETT59_RS02665 (ETT59_02665) - 510015..510656 (+) 642 WP_002983671.1 response regulator transcription factor -
  ETT59_RS02670 (ETT59_02670) - 510693..512093 (+) 1401 WP_053308255.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  ETT59_RS02675 (ETT59_02675) - 512093..512470 (+) 378 WP_032464495.1 S1 RNA-binding domain-containing protein -
  ETT59_RS02680 (ETT59_02680) cysK 512488..513429 (-) 942 WP_011528853.1 cysteine synthase A -
  ETT59_RS02685 (ETT59_02685) - 513557..514189 (-) 633 WP_002983676.1 YigZ family protein -
  ETT59_RS02690 (ETT59_02690) comFA/cflA 514244..515569 (+) 1326 WP_053308256.1 DEAD/DEAH box helicase Machinery gene
  ETT59_RS02695 (ETT59_02695) - 515541..516206 (+) 666 WP_053308257.1 ComF family protein -
  ETT59_RS02700 (ETT59_02700) hpf 516286..516834 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ETT59_RS02705 (ETT59_02705) - 517389..518606 (-) 1218 WP_002835202.1 tyrosine-type recombinase/integrase -
  ETT59_RS02710 (ETT59_02710) - 518688..518891 (-) 204 WP_000814512.1 excisionase -
  ETT59_RS09135 - 518875..519125 (+) 251 Protein_469 hypothetical protein -
  ETT59_RS02720 (ETT59_02720) - 519351..519581 (-) 231 WP_000857133.1 helix-turn-helix domain-containing protein -
  ETT59_RS02725 (ETT59_02725) - 519578..520000 (-) 423 WP_000804885.1 sigma-70 family RNA polymerase sigma factor -
  ETT59_RS02735 (ETT59_02735) - 520505..520858 (+) 354 WP_001227347.1 helix-turn-helix transcriptional regulator -
  ETT59_RS02740 (ETT59_02740) - 520918..521085 (-) 168 WP_000336323.1 cysteine-rich KTR domain-containing protein -
  ETT59_RS02745 (ETT59_02745) tet(M) 521204..523123 (-) 1920 WP_015646599.1 tetracycline resistance ribosomal protection protein Tet(M) -
  ETT59_RS09310 - 523139..523186 (-) 48 Protein_475 hypothetical protein -
  ETT59_RS02755 (ETT59_02755) - 523500..524435 (-) 936 WP_001224320.1 conjugal transfer protein -
  ETT59_RS02760 (ETT59_02760) - 524432..525433 (-) 1002 WP_136306020.1 lysozyme family protein -
  ETT59_RS02765 (ETT59_02765) - 525430..527613 (-) 2184 WP_002345008.1 CD3337/EF1877 family mobilome membrane protein -
  ETT59_RS02770 (ETT59_02770) - 527616..530063 (-) 2448 WP_000331148.1 ATP-binding protein -
  ETT59_RS02775 (ETT59_02775) - 530047..530439 (-) 393 WP_002345009.1 conjugal transfer protein -
  ETT59_RS02780 (ETT59_02780) - 530526..531023 (-) 498 WP_002345010.1 antirestriction protein ArdA -
  ETT59_RS02785 (ETT59_02785) - 531142..531363 (-) 222 WP_001009056.1 hypothetical protein -
  ETT59_RS02790 (ETT59_02790) mobT 531406..532611 (-) 1206 WP_015646596.1 MobT family relaxase -
  ETT59_RS02795 (ETT59_02795) - 532646..532759 (-) 114 WP_078102635.1 conjugal transfer protein -
  ETT59_RS02800 (ETT59_02800) - 532788..533225 (-) 438 WP_015646595.1 hypothetical protein -
  ETT59_RS02805 (ETT59_02805) - 533231..534625 (-) 1395 WP_015646594.1 FtsK/SpoIIIE domain-containing protein -
  ETT59_RS02810 (ETT59_02810) - 534665..535051 (-) 387 WP_000985016.1 YdcP family protein -
  ETT59_RS02815 (ETT59_02815) - 535067..535381 (-) 315 WP_000420680.1 YdcP family protein -

Sequence

Protein


Download         Length: 632 a.a.        Molecular weight: 68814.04 Da        Isoelectric Point: 7.0888

>NTDB_id=341693 ETT59_RS02650 WP_111681221.1 506308..508206(+) (pknB) [Streptococcus pyogenes strain emm124]
MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVLRTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIG
EEDGQQFLVMEYVDGADLKRYIQNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIEENHNVP
QALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYNRSREHKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESR
LDQTNQTDALQPPTKKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDVGLKVGKIR
QIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDYQEAMNSLIETYGVPKSKIKIERIVTNEYPE
NTVISQSPSAGDKFNPNGKSKITLSVAVSDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSS
KAIVSGQSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSPSETTAQTP

Nucleotide


Download         Length: 1899 bp        

>NTDB_id=341693 ETT59_RS02650 WP_111681221.1 506308..508206(+) (pknB) [Streptococcus pyogenes strain emm124]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGCATTCTGAAATCTATTGGCCGCGGTGGTATGGCGGATGTTTA
TTTAGCAAATGACTTGATCTTGGATAATGAAGACGTTGCAATCAAGGTCTTGCGTACCAATTATCAAACAGATCAGGTAG
CAGTTGCGCGTTTCCAACGAGAAGCGCGAGCCATGGCTGAATTGAACCATCCCAATATTGTTGCCATCCGGGATATAGGT
GAAGAAGACGGACAGCAATTTTTAGTAATGGAATATGTGGATGGTGCTGACCTAAAGAGATACATTCAAAATCATGCTCC
ATTATCTAATAATGAAGTGGTTAGAATTATGGAAGAAGTCCTTTCTGCTATGACTTTAGCCCACCAAAAAGGAATTGTAC
ACAGAGATTTAAAACCTCAAAATATCCTACTAACTAAGGAGGGTGTTGTTAAAGTAACTGATTTCGGCATCGCAGTAGCC
TTTGCAGAAACAAGCTTGACACAAACTAATTCGATGTTAGGCAGTGTTCATTACTTGTCTCCAGAACAGGCTCGCGGCTC
CAAAGCGACGATTCAAAGTGATATTTATGCGATGGGGATTATGCTCTTTGAGATGTTGACAGGCCATATCCCTTATGATG
GCGATAGTGCTGTTACGATTGCCTTGCAACATTTTCAAAAGCCTCTTCCATCTATTATCGAGGAGAACCACAATGTGCCA
CAAGCTTTGGAGAATGTTGTTATTCGAGCAACAGCCAAGAAATTAAGTGATCGTTACGGGTCAACCTTTGAAATGAGTCG
TGACTTAATGACGGCGCTTAGTTATAATCGTAGTCGGGAGCATAAGATTATCTTTGAGGATGTTGAAAGTACCAAACCCC
TCCCCAAAGTGGCCTCAGGTCCCACCGCTTCTGTAAAATTGTCTCCCCCTACCCCAACAGTGTTAACACAGGAAAGTCGA
TTAGATCAAACTAATCAAACAGATGCTTTACAGCCCCCCACCAAAAAGAAAAAAAGTGGTCGTTTTTTAGGTACTTTATT
CAAAATTCTTTTTTCTTTCTTTATTGTAGGTGTAGCACTCTTTACTTATCTTATACTAACTAAACCAACTTCTGTGAAAG
TTCCTAATGTAGCAGGCACTAGCCTTAAAGTTGCCAAACAAGAACTGTATGATGTTGGGCTAAAAGTGGGTAAAATCAGG
CAAATTGAGAGTGATACGGTTGCTGAGGGAAATGTAGTTAGAACAGATCCTAAAGCAGGAACAGCTAAGAGGCAAGGCTC
AAGCATTACGCTTTATGTGTCAATTGGAAACAAAGGTTTTGACATGGAAAACTACAAAGGACTAGATTATCAAGAAGCTA
TGAATAGTTTGATAGAAACTTATGGTGTTCCAAAATCAAAAATCAAAATTGAGCGCATTGTAACTAATGAATATCCTGAA
AATACAGTCATCAGTCAATCGCCAAGTGCGGGTGATAAATTTAATCCAAACGGAAAGTCTAAAATTACGCTCAGTGTTGC
TGTTAGTGATACGATCACTATGCCTATGGTAACAGAATATAGTTATGCAGATGCAGTCAATACCTTAACAGCTTTAGGTA
TAGATGCATCTAGAATAAAAGCTTATGTGCCAAGCTCTAGCTCAGCAACGGGCTTTGTGCCAATTCATTCTCCTAGTTCT
AAAGCTATTGTCAGTGGTCAATCTCCTTACTATGGAACGTCTTTGAGTCTGTCTGATAAAGGAGAGATTAGTCTTTACCT
TTATCCAGAAGAAACACACTCTTCTAGTAGCTCATCGAGTTCAACGTCAAGTTCAAACAGTTCTTCAACAAATGATAGTA
CTGCACCAGGTAGCAACACTGAATTAAGCCCATCAGAAACTACTGCTCAAACACCTTAA

Domains

Predicted by InterproScan.

PASTA domain (PASTA)

(372-434)

PASTA domain (PASTA)

(441-506)

PASTA domain (PASTA)

(512-588)

Protein kinase domain (Pkinase)

(12-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

65.085

93.354

0.608
  stkP/pknB Streptococcus salivarius strain HSISS4

61.345

94.146

0.578
  stkP Streptococcus pneumoniae TIGR4

57.364

81.646

0.468
  stkP Streptococcus pneumoniae D39

57.171

81.646

0.467
  stkP Streptococcus pneumoniae R6

57.171

81.646

0.467
  stkP Streptococcus pneumoniae Rx1

56.977

81.646

0.465

Multiple sequence alignment