Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ETT59_RS02690 Genome accession   NZ_CP035440
Coordinates   514244..515569 (+) Length   441 a.a.
NCBI ID   WP_053308256.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm124     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 490991..535890 514244..515569 within 0


Gene organization within MGE regions


Location: 490991..535890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT59_RS02580 (ETT59_02580) - 491574..492056 (-) 483 WP_002988938.1 S-ribosylhomocysteine lyase -
  ETT59_RS02585 (ETT59_02585) - 492130..493461 (-) 1332 WP_136276603.1 GntP family permease -
  ETT59_RS02590 (ETT59_02590) - 493532..494311 (-) 780 WP_023611644.1 3-hydroxybutyrate dehydrogenase -
  ETT59_RS02595 (ETT59_02595) - 494344..495003 (-) 660 WP_002988946.1 3-oxoacid CoA-transferase subunit B -
  ETT59_RS02600 (ETT59_02600) - 495005..495655 (-) 651 WP_002983642.1 CoA transferase subunit A -
  ETT59_RS02605 (ETT59_02605) - 495679..496866 (-) 1188 WP_136300706.1 acetyl-CoA C-acetyltransferase -
  ETT59_RS02610 (ETT59_02610) - 497067..497960 (+) 894 WP_129321336.1 LysR family transcriptional regulator -
  ETT59_RS02615 (ETT59_02615) - 498090..499697 (+) 1608 WP_014635620.1 ribonuclease Y -
  ETT59_RS02620 (ETT59_02620) gmk 499807..500442 (+) 636 WP_002983649.1 guanylate kinase -
  ETT59_RS02625 (ETT59_02625) rpoZ 500458..500775 (+) 318 WP_014635619.1 DNA-directed RNA polymerase subunit omega -
  ETT59_RS02630 (ETT59_02630) - 500840..503224 (+) 2385 WP_136276607.1 primosomal protein N' -
  ETT59_RS02635 (ETT59_02635) fmt 503286..504221 (+) 936 WP_053308253.1 methionyl-tRNA formyltransferase -
  ETT59_RS02640 (ETT59_02640) rsmB 504211..505533 (+) 1323 WP_136306021.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ETT59_RS02645 (ETT59_02645) - 505571..506311 (+) 741 WP_136276609.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ETT59_RS02650 (ETT59_02650) pknB 506308..508206 (+) 1899 WP_111681221.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ETT59_RS02655 (ETT59_02655) liaF 508329..509021 (+) 693 WP_011054833.1 cell wall-active antibiotics response protein LiaF -
  ETT59_RS02660 (ETT59_02660) - 509018..510022 (+) 1005 WP_002991890.1 sensor histidine kinase -
  ETT59_RS02665 (ETT59_02665) - 510015..510656 (+) 642 WP_002983671.1 response regulator transcription factor -
  ETT59_RS02670 (ETT59_02670) - 510693..512093 (+) 1401 WP_053308255.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  ETT59_RS02675 (ETT59_02675) - 512093..512470 (+) 378 WP_032464495.1 S1 RNA-binding domain-containing protein -
  ETT59_RS02680 (ETT59_02680) cysK 512488..513429 (-) 942 WP_011528853.1 cysteine synthase A -
  ETT59_RS02685 (ETT59_02685) - 513557..514189 (-) 633 WP_002983676.1 YigZ family protein -
  ETT59_RS02690 (ETT59_02690) comFA/cflA 514244..515569 (+) 1326 WP_053308256.1 DEAD/DEAH box helicase Machinery gene
  ETT59_RS02695 (ETT59_02695) - 515541..516206 (+) 666 WP_053308257.1 ComF family protein -
  ETT59_RS02700 (ETT59_02700) hpf 516286..516834 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ETT59_RS02705 (ETT59_02705) - 517389..518606 (-) 1218 WP_002835202.1 tyrosine-type recombinase/integrase -
  ETT59_RS02710 (ETT59_02710) - 518688..518891 (-) 204 WP_000814512.1 excisionase -
  ETT59_RS09135 - 518875..519125 (+) 251 Protein_469 hypothetical protein -
  ETT59_RS02720 (ETT59_02720) - 519351..519581 (-) 231 WP_000857133.1 helix-turn-helix domain-containing protein -
  ETT59_RS02725 (ETT59_02725) - 519578..520000 (-) 423 WP_000804885.1 sigma-70 family RNA polymerase sigma factor -
  ETT59_RS02735 (ETT59_02735) - 520505..520858 (+) 354 WP_001227347.1 helix-turn-helix transcriptional regulator -
  ETT59_RS02740 (ETT59_02740) - 520918..521085 (-) 168 WP_000336323.1 cysteine-rich KTR domain-containing protein -
  ETT59_RS02745 (ETT59_02745) tet(M) 521204..523123 (-) 1920 WP_015646599.1 tetracycline resistance ribosomal protection protein Tet(M) -
  ETT59_RS09310 - 523139..523186 (-) 48 Protein_475 hypothetical protein -
  ETT59_RS02755 (ETT59_02755) - 523500..524435 (-) 936 WP_001224320.1 conjugal transfer protein -
  ETT59_RS02760 (ETT59_02760) - 524432..525433 (-) 1002 WP_136306020.1 lysozyme family protein -
  ETT59_RS02765 (ETT59_02765) - 525430..527613 (-) 2184 WP_002345008.1 CD3337/EF1877 family mobilome membrane protein -
  ETT59_RS02770 (ETT59_02770) - 527616..530063 (-) 2448 WP_000331148.1 ATP-binding protein -
  ETT59_RS02775 (ETT59_02775) - 530047..530439 (-) 393 WP_002345009.1 conjugal transfer protein -
  ETT59_RS02780 (ETT59_02780) - 530526..531023 (-) 498 WP_002345010.1 antirestriction protein ArdA -
  ETT59_RS02785 (ETT59_02785) - 531142..531363 (-) 222 WP_001009056.1 hypothetical protein -
  ETT59_RS02790 (ETT59_02790) mobT 531406..532611 (-) 1206 WP_015646596.1 MobT family relaxase -
  ETT59_RS02795 (ETT59_02795) - 532646..532759 (-) 114 WP_078102635.1 conjugal transfer protein -
  ETT59_RS02800 (ETT59_02800) - 532788..533225 (-) 438 WP_015646595.1 hypothetical protein -
  ETT59_RS02805 (ETT59_02805) - 533231..534625 (-) 1395 WP_015646594.1 FtsK/SpoIIIE domain-containing protein -
  ETT59_RS02810 (ETT59_02810) - 534665..535051 (-) 387 WP_000985016.1 YdcP family protein -
  ETT59_RS02815 (ETT59_02815) - 535067..535381 (-) 315 WP_000420680.1 YdcP family protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49757.20 Da        Isoelectric Point: 10.0339

>NTDB_id=341695 ETT59_RS02690 WP_053308256.1 514244..515569(+) (comFA/cflA) [Streptococcus pyogenes strain emm124]
MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGNPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSSMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=341695 ETT59_RS02690 WP_053308256.1 514244..515569(+) (comFA/cflA) [Streptococcus pyogenes strain emm124]
ATGGAGTCTATTGAAAATAGTTATGGGCGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAGGCAACTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAGTTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTTTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCAGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCAATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTAGTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae D39

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae R6

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae TIGR4

54.177

95.011

0.515
  comFA/cflA Streptococcus mitis NCTC 12261

52.844

95.692

0.506
  comFA/cflA Streptococcus mitis SK321

51.896

95.692

0.497
  comFA Lactococcus lactis subsp. cremoris KW2

47.368

90.476

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.851

91.61

0.365

Multiple sequence alignment