Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FOB75_RS11850 Genome accession   NZ_CP044062
Coordinates   2347606..2349108 (-) Length   500 a.a.
NCBI ID   WP_150334460.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FDAARGOS_667     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2342606..2354108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB75_RS11820 (FOB75_11820) gspK 2343208..2344218 (-) 1011 WP_005478666.1 type II secretion system minor pseudopilin GspK -
  FOB75_RS11825 (FOB75_11825) gspJ 2344211..2344894 (-) 684 WP_020839934.1 type II secretion system minor pseudopilin GspJ -
  FOB75_RS11830 (FOB75_11830) gspI 2344881..2345270 (-) 390 WP_029855934.1 type II secretion system minor pseudopilin GspI -
  FOB75_RS11835 (FOB75_11835) gspH 2345257..2345853 (-) 597 WP_150334459.1 type II secretion system minor pseudopilin GspH -
  FOB75_RS11840 (FOB75_11840) gspG 2345884..2346327 (-) 444 WP_005465191.1 type II secretion system major pseudopilin GspG -
  FOB75_RS11845 (FOB75_11845) gspF 2346389..2347606 (-) 1218 WP_017449057.1 type II secretion system inner membrane protein GspF -
  FOB75_RS11850 (FOB75_11850) pilB/pilB1 2347606..2349108 (-) 1503 WP_150334460.1 type II secretion system ATPase GspE Machinery gene
  FOB75_RS11855 (FOB75_11855) gspD 2349108..2351132 (-) 2025 WP_020839932.1 type II secretion system secretin GspD -
  FOB75_RS11860 (FOB75_11860) gspC 2351169..2352092 (-) 924 WP_005465330.1 type II secretion system protein GspC -
  FOB75_RS11865 (FOB75_11865) hslR 2352348..2352734 (+) 387 WP_005465328.1 ribosome-associated heat shock protein Hsp15 -
  FOB75_RS11870 (FOB75_11870) hslO 2352766..2353641 (+) 876 WP_005465326.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 55716.85 Da        Isoelectric Point: 5.9227

>NTDB_id=340152 FOB75_RS11850 WP_150334460.1 2347606..2349108(-) (pilB/pilB1) [Vibrio parahaemolyticus strain FDAARGOS_667]
MVDMLDTAPSMRRLPFSFANRFKLVLETEHPERPPILYYVEPLNPAALVEVRRVLKRSFVPQAIDKESFDKKLTEAYQRD
SSEARQLMEDIGADSDDFFSLAEELPHDEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGVLRE
VLTPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTP
SVHDSFRHLIGRPHGIILVTGPTGSGKSTTLYAGLQEINSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAIL
RQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCSDCKEP
YEADSEQKKLFGMAESESLILHRAKGCEACNQKGYRGRTGIHELVLVDETVQELIHREAGEQEVEKAVRNHTPSIRSDGL
SKVRRGITSLEEVMRVTKEA

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=340152 FOB75_RS11850 WP_150334460.1 2347606..2349108(-) (pilB/pilB1) [Vibrio parahaemolyticus strain FDAARGOS_667]
ATGGTAGATATGTTAGACACTGCGCCAAGTATGCGTCGTCTGCCATTCAGTTTTGCCAATCGCTTTAAACTGGTTTTGGA
GACTGAGCATCCAGAGCGTCCTCCAATTCTTTACTACGTGGAGCCGCTAAATCCTGCGGCTCTGGTTGAAGTACGTCGCG
TGCTTAAGCGCAGTTTCGTGCCTCAAGCCATCGACAAAGAATCCTTCGATAAAAAGCTGACTGAAGCGTATCAACGTGAT
TCATCAGAAGCTCGTCAATTGATGGAAGATATCGGTGCCGACAGTGACGATTTCTTCTCGTTGGCAGAAGAGCTACCGCA
TGACGAGGATTTGTTAGAGTCGGAAGACGATGCGCCAATCATCAAATTGATCAATGCTATGTTGGGTGAAGCGATCAAAG
AAGGTGCGTCCGATATCCATATCGAAACGTTTGAAAAGACGCTTTCTATCCGCTTCCGTGTCGACGGCGTTCTGCGTGAA
GTCTTGACGCCGAGTCGTAAGCTTGCGCCGTTGTTGGTGTCGCGTGTGAAGGTTATGGCGAAGCTCGATATTGCTGAAAA
GCGTGTGCCTCAAGATGGTCGTATCTCTCTACGTATTGGTGGCCGAGCGGTCGACGTGCGTGTTTCTACTATGCCATCCT
CTCACGGTGAACGAGTGGTAATGCGTCTGTTAGATAAAAACGCAACGCGCCTTGACCTGCATAGTTTGGGCATGACTCCT
TCGGTACACGACAGCTTCCGTCACCTCATTGGTCGCCCACACGGCATCATCTTGGTGACCGGCCCTACGGGTTCTGGTAA
ATCGACCACTTTGTACGCCGGCTTGCAAGAAATCAACAGCAACGAGCGCAACATTCTGACGGTGGAAGACCCAATCGAAT
TTGATATCGATGGCATTGGCCAGACCCAAGTTAATCCGAAAGTCGACATGACATTTGCGCGTGGTCTACGTGCGATCTTA
CGTCAGGACCCGGATGTGGTGATGGTTGGTGAGATCCGTGACTTAGAAACCGCGCAAATTGCGGTGCAAGCGTCTTTGAC
TGGTCACTTGGTAATGTCGACGCTGCATACCAACACCGCTGTTGGTGCGATCACGCGTCTGCGTGATATGGGCATTGAAC
CATTTTTGATCTCCTCTTCACTGCTTGGTGTGCTAGCGCAGCGTCTGGTTCGTACGCTTTGTTCTGACTGTAAAGAACCT
TACGAAGCGGACAGCGAACAGAAAAAATTGTTTGGCATGGCAGAAAGTGAATCGCTCATTCTACATCGCGCGAAAGGCTG
TGAAGCGTGTAACCAAAAAGGTTATCGCGGACGTACCGGCATTCATGAATTAGTGCTTGTCGATGAAACGGTTCAAGAGC
TGATTCACCGCGAAGCAGGTGAGCAAGAAGTGGAAAAAGCCGTTCGTAATCATACGCCAAGTATTCGTAGTGATGGTCTA
AGCAAAGTACGTCGTGGTATTACGTCACTTGAAGAAGTGATGCGAGTGACGAAGGAAGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(126-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.939

100

0.45
  pilB Legionella pneumophila strain ERS1305867

43.763

97.8

0.428
  pilF Thermus thermophilus HB27

44.161

94.2

0.416
  pilB Acinetobacter baumannii D1279779

42.62

96.2

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

46.455

81.8

0.38
  pilB Vibrio campbellii strain DS40M4

46.455

81.8

0.38
  pilB Vibrio cholerae strain A1552

47.475

79.2

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.692

78

0.372
  pilF Neisseria gonorrhoeae MS11

46.193

78.8

0.364
  pilB Acinetobacter baylyi ADP1

46.53

77.8

0.362