Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EO087_RS15025 Genome accession   NZ_CP035300
Coordinates   3273942..3275666 (-) Length   574 a.a.
NCBI ID   WP_128899572.1    Uniprot ID   A0A410ULK7
Organism   Dyella sp. M7H15-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3268942..3280666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO087_RS15005 (EO087_15005) coaE 3269215..3269865 (-) 651 WP_128899568.1 dephospho-CoA kinase -
  EO087_RS15010 (EO087_15010) - 3269868..3271580 (-) 1713 WP_128899569.1 phosphoethanolamine transferase -
  EO087_RS15015 (EO087_15015) - 3271591..3272448 (-) 858 WP_128899570.1 A24 family peptidase -
  EO087_RS15020 (EO087_15020) pilC 3272524..3273792 (-) 1269 WP_128899571.1 type II secretion system F family protein Machinery gene
  EO087_RS15025 (EO087_15025) pilB 3273942..3275666 (-) 1725 WP_128899572.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EO087_RS15030 (EO087_15030) - 3275934..3276788 (+) 855 WP_128899573.1 glycosyltransferase family 2 protein -
  EO087_RS15035 (EO087_15035) - 3276793..3277908 (+) 1116 WP_128899574.1 UDP-N-acetylglucosamine 2-epimerase -
  EO087_RS15040 (EO087_15040) - 3277978..3279000 (+) 1023 WP_164931866.1 glycosyltransferase family 4 protein -
  EO087_RS15045 (EO087_15045) - 3279044..3279571 (+) 528 WP_128899576.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62773.45 Da        Isoelectric Point: 6.8339

>NTDB_id=339064 EO087_RS15025 WP_128899572.1 3273942..3275666(-) (pilB) [Dyella sp. M7H15-1]
MSSQPQPTTHSLSGMARRLVSEGLLPEADVRRAVLESSQQKKALTVWLLDRGLLDSAQLAQVSSAEFGMPMLDVMALDPS
SMPLSLISQELISKHQALPLFKRGKRLFVGIADPMQLHALDEIKFHSNCMVEPILVERSQLGRAIENAISALNNVTPDLG
GGDLDDLALESRDDEADTGGGIDATANDDAPVVKFVNKILVDAIRRGASDIHFEPYETQYRVRLRMDGMLRAVASPPMKL
SNRISSRLKVMAGLDIAERRVPQDGRIKLNLSKSHAMDFRVSTLPTLFGEKIVLRILDSSSAKLGIDKLGYEDQQKKLYL
DAIHKPYGMVLVTGPTGSGKTVSLYTALNILNTTERNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALRSFLRQDPDVI
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPYNITSSVTLVIAQRLARRLHSCKKQMTLPAKTL
LAAGFTQEEIDAGMTIYEAVGCAECNEGYKGRVGIYQVMPMVEDIQKIVLEGGNALQIAEAARKIGIDDLRASALLKVKN
GITSLAEIDRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=339064 EO087_RS15025 WP_128899572.1 3273942..3275666(-) (pilB) [Dyella sp. M7H15-1]
ATGTCATCACAACCGCAGCCCACCACACATAGCTTGTCAGGCATGGCCCGCCGCCTGGTGTCCGAAGGACTGCTTCCAGA
GGCCGATGTCCGTAGGGCCGTGCTCGAATCGAGCCAGCAAAAGAAAGCGCTGACCGTTTGGCTCTTGGACCGGGGCCTGT
TGGACAGCGCACAGCTCGCCCAAGTCTCATCCGCCGAATTCGGCATGCCCATGCTGGATGTCATGGCGCTCGATCCATCC
AGTATGCCTTTAAGCCTTATCAGCCAGGAGTTGATAAGCAAGCATCAGGCGCTCCCCCTTTTCAAACGAGGCAAACGTCT
GTTTGTCGGTATTGCCGACCCGATGCAGCTACATGCACTGGATGAGATCAAGTTCCACTCCAATTGCATGGTGGAACCAA
TTCTGGTTGAAAGGTCTCAGTTGGGACGCGCGATTGAGAATGCCATAAGTGCGCTCAATAACGTTACCCCCGACCTGGGC
GGCGGTGACCTGGACGACTTGGCCCTAGAGTCCAGGGACGATGAAGCAGATACGGGGGGTGGTATTGACGCCACCGCAAA
TGACGACGCCCCCGTCGTCAAATTTGTGAACAAGATTCTGGTGGATGCCATTCGCCGTGGCGCCTCGGACATCCATTTCG
AACCTTATGAGACCCAGTACCGGGTGCGGTTGCGAATGGACGGCATGTTACGAGCTGTTGCCAGTCCCCCGATGAAACTG
TCCAACCGCATTTCTTCCCGCTTGAAAGTGATGGCCGGACTGGATATCGCCGAGCGCCGCGTACCTCAAGACGGGCGCAT
CAAGCTCAATCTTTCCAAAAGCCACGCAATGGATTTTCGCGTCAGCACCTTACCAACCCTGTTTGGCGAGAAGATTGTCT
TGCGTATCCTAGATAGTTCTTCAGCCAAGCTAGGCATCGACAAGCTGGGTTATGAGGACCAACAAAAAAAGCTTTACCTT
GACGCCATCCACAAACCGTATGGGATGGTCTTGGTCACCGGCCCCACGGGTTCTGGCAAAACCGTTTCGCTCTATACCGC
GCTGAACATTCTCAATACCACCGAACGCAATATTTCCACCGTGGAAGACCCGGTGGAAATTCGCGTCGAAGGTATCAATC
AGGTGCAGCAAAACACCAAGCGAGGCATGACCTTCGCAGCGGCCCTGCGCTCCTTCCTGCGCCAAGACCCTGACGTGATC
ATGGTGGGCGAAATCCGCGATCTGGAAACGGCCGAAATTGCCATCAAGGCGGCTCAGACAGGCCACATGGTGTTGTCGAC
CCTGCACACTAATGATGCACCGCAAACCATCTCGCGACTCATGAACATGGGTATCGCCCCCTACAACATCACCTCATCAG
TGACACTGGTTATCGCTCAGCGCTTGGCACGCCGCCTACATAGTTGCAAAAAGCAAATGACCCTGCCGGCCAAGACCTTG
CTTGCCGCTGGCTTTACCCAGGAAGAGATCGATGCAGGAATGACGATCTATGAGGCGGTTGGCTGCGCGGAATGCAACGA
GGGCTATAAAGGACGCGTGGGTATCTATCAAGTTATGCCTATGGTGGAAGATATTCAGAAGATCGTGCTGGAGGGCGGAA
ATGCTCTGCAAATCGCCGAAGCCGCCCGTAAAATCGGCATCGATGACCTACGCGCTTCAGCCTTGTTGAAGGTCAAAAAT
GGGATAACCAGCCTTGCCGAAATTGATCGCGTAACCAAAGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-147)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A410ULK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.799

99.826

0.547
  pilB Acinetobacter baylyi ADP1

54.306

99.129

0.538
  pilB Legionella pneumophila strain ERS1305867

51.049

99.652

0.509
  pilB Vibrio cholerae strain A1552

49.293

98.606

0.486
  pilF Neisseria gonorrhoeae MS11

47.652

100

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

50.562

93.031

0.47
  pilB Vibrio campbellii strain DS40M4

50.187

93.031

0.467
  pilF Thermus thermophilus HB27

38.283

97.387

0.373

Multiple sequence alignment