Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EQH16_RS09975 Genome accession   NZ_CP035264
Coordinates   1910394..1911410 (-) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901921     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1905394..1916410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH16_RS11155 - 1905471..1905602 (-) 132 WP_000768904.1 hypothetical protein -
  EQH16_RS09935 (EQH16_10425) - 1905603..1906793 (-) 1191 WP_000167766.1 acetate kinase -
  EQH16_RS09940 (EQH16_10430) comYH 1906844..1907797 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  EQH16_RS09945 (EQH16_10435) - 1907858..1908452 (-) 595 Protein_1962 class I SAM-dependent methyltransferase -
  EQH16_RS09950 (EQH16_10440) comGG/cglG 1908589..1909002 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQH16_RS09955 (EQH16_10445) comGF/cglF 1908980..1909441 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQH16_RS09960 (EQH16_10450) comGE/cglE 1909404..1909706 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQH16_RS09965 (EQH16_10455) comGD/cglD 1909669..1910073 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQH16_RS09970 (EQH16_10460) comGC/cglC 1910066..1910392 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  EQH16_RS09975 (EQH16_10465) comGB/cglB 1910394..1911410 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH16_RS09980 (EQH16_10470) comGA/cglA/cilD 1911358..1912299 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH16_RS09985 (EQH16_10475) - 1912375..1912740 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQH16_RS09990 (EQH16_10480) - 1912891..1913949 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  EQH16_RS09995 (EQH16_10485) nagA 1914112..1915263 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=338651 EQH16_RS09975 WP_074196785.1 1910394..1911410(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901921]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=338651 EQH16_RS09975 WP_074196785.1 1910394..1911410(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901921]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus pneumoniae R6

100

100

1
  comGB/cglB Streptococcus pneumoniae TIGR4

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.547

94.083

0.541
  comYB Streptococcus mutans UA159

57.547

94.083

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment