Detailed information NTG0338151

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: EQH22_RS05855
• Genome accession: NZ_CP035258
• Coordinates: 1181695..1182543 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705304.1
• Uniprot ID: A0A0E7P1X9
• Organism: Streptococcus pneumoniae strain TVO_1901927
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 1176695..1187543

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH22_RS05840 (EQH22_06135)	-	1176936..1177295 (-)	360	WP_001237219.1	YbaN family protein	-
EQH22_RS05845 (EQH22_06140)	topA	1177516..1179603 (-)	2088	WP_000179638.1	type I DNA topoisomerase	-
EQH22_RS05850 (EQH22_06145)	-	1179770..1180813 (-)	1044	WP_001812883.1	TIGR00341 family protein	-
EQH22_RS10915	-	1181390..1181512 (+)	123	WP_001814242.1	hypothetical protein	-
EQH22_RS05855 (EQH22_06150)	dprA/cilB/dalA	1181695..1182543 (-)	849	WP_000705304.1	DNA-processing protein DprA	Machinery gene
EQH22_RS05860 (EQH22_06155)	-	1182640..1183329 (-)	690	WP_000643970.1	sugar phosphate nucleotidyltransferase	-
EQH22_RS05865 (EQH22_06160)	-	1183341..1184219 (-)	879	WP_000837609.1	DMT family transporter	-
EQH22_RS05870 (EQH22_06165)	licA	1184209..1185078 (-)	870	WP_000411216.1	choline kinase LicA	-
EQH22_RS05875 (EQH22_06170)	-	1185095..1186117 (-)	1023	WP_000609885.1	ribitol-5-phosphate dehydrogenase	-
EQH22_RS05880 (EQH22_06175)	-	1186122..1186829 (-)	708	WP_000638502.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31076.75 Da
• Isoelectric Point: 5.4047

>NTDB_id=338151 EQH22_RS05855 WP_000705304.1 1181695..1182543(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901927]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=338151 EQH22_RS05855 WP_000705304.1 1181695..1182543(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901927]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTCTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGCTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

3D structure

• AlphaFold DB: A0A0E7P1X9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae D39	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae R6	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	99.645	100	0.996
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	98.227	100	0.982
dprA/cilB/dalA	Streptococcus mitis SK321	97.518	100	0.975
dprA	Streptococcus mutans UA159	63.214	99.291	0.628
dprA	Lactococcus lactis subsp. cremoris KW2	55.357	99.291	0.55
dprA	Staphylococcus aureus N315	37.456	100	0.376