Detailed information NTG0337285

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: EQH36_RS05940
• Genome accession: NZ_CP035244
• Coordinates: 1188592..1189440 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705324.1
• Uniprot ID: A0A4K9QSJ2
• Organism: Streptococcus pneumoniae strain TVO_1901945
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 1183592..1194440

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH36_RS05925 (EQH36_06230)	-	1183946..1184305 (-)	360	WP_001237219.1	YbaN family protein	-
EQH36_RS05930 (EQH36_06235)	topA	1184411..1186498 (-)	2088	WP_000179650.1	type I DNA topoisomerase	-
EQH36_RS05935 (EQH36_06240)	-	1186665..1187708 (-)	1044	WP_001812957.1	TIGR00341 family protein	-
EQH36_RS10855	-	1188286..1188408 (+)	123	WP_263969828.1	hypothetical protein	-
EQH36_RS05940 (EQH36_06245)	dprA/cilB/dalA	1188592..1189440 (-)	849	WP_000705324.1	DNA-processing protein DprA	Machinery gene
EQH36_RS05945 (EQH36_06250)	-	1189536..1190225 (-)	690	WP_000643967.1	sugar phosphate nucleotidyltransferase	-
EQH36_RS05950 (EQH36_06255)	-	1190237..1191115 (-)	879	WP_000837609.1	DMT family transporter	-
EQH36_RS05955 (EQH36_06260)	licA	1191105..1191974 (-)	870	WP_000411201.1	choline kinase LicA	-
EQH36_RS05960 (EQH36_06265)	-	1191991..1193013 (-)	1023	WP_000609915.1	ribitol-5-phosphate dehydrogenase	-
EQH36_RS05965 (EQH36_06270)	-	1193018..1193725 (-)	708	WP_000638497.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31150.79 Da
• Isoelectric Point: 5.2061

>NTDB_id=337285 EQH36_RS05940 WP_000705324.1 1188592..1189440(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901945]
MKITNYEIYKLKKSSLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPDSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=337285 EQH36_RS05940 WP_000705324.1 1188592..1189440(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901945]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAAGTTTGACCAATCAACAGATTTTGAAAGTTCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGATAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

3D structure

• AlphaFold DB: A0A4K9QSJ2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	99.291	100	0.993
dprA/cilB/dalA	Streptococcus pneumoniae D39	99.291	100	0.993
dprA/cilB/dalA	Streptococcus pneumoniae R6	99.291	100	0.993
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	99.291	100	0.993
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	97.872	100	0.979
dprA/cilB/dalA	Streptococcus mitis SK321	97.163	100	0.972
dprA	Streptococcus mutans UA159	62.5	99.291	0.621
dprA	Lactococcus lactis subsp. cremoris KW2	55	99.291	0.546
dprA	Staphylococcus aureus N315	37.456	100	0.376